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ZNF146 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF146
Gene Name:  zinc finger protein 146
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH025774.01L
Keyword: potential short variant
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: Short variant or partial clone comparing to NM_007145
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00022488

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 516nts         Open reading frame : 1 to 516
Reference Sequence Alignment

ATGTCACACCTCAGCCAGCAGAAAATTTACAGTGGGGAAAACCCCTTTGCCTGTAAGGTA
TGTGGAAAAGTCTTCAGCCACAAATCAAACCTCACTGAGCATGAGCATTTTCACACGAGA
GAAAACCTTTTGAATGTAACGAGTGTGGAAAAGCCTTTAGCCAAAAGCAGTATGTCATTA
AACATCAGAACACCCATACTGGCGAGAAGCTTTTCGAATGTAATGAATGTGGAAAATCAT
TTAGCCAGAAGGAAAACCTCCTTACGCACCAGAAAATTCACACTGGAGAAAAACCTTTTG
AGTGTAAAGATTGCGGGAAAGCTTTCATTCAGAAGTCAAACCTCATCAGACACCAGAGAA
CTCACACAGGAGAGAAGCCCTTTGTATGTAAGGAGTGTGGAAAAACCTTCAGTGGCAAAT
CCAACCTTACTGAGCATGAGAAAATCCATATTGGAGAGAAGCCTTTTAAATGTAGTGAAT
GTGGAACAGCCTTTGGCCAGAAGAAGTACCTCATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1510
Start on reference sequence 995
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF146
Symbol Nomenclature : ZNF146
SYNONYM : OZF
Designation : only zinc finger protein|zinc finger protein OZF
Full Nomenclature : zinc finger protein 146
GENEID : 7705
HGNC : 12931
MIM : 601505
Vega : OTTHUMG00000180511
Target GenBank: BC005154

 

Reference Sequence Alignment

  

HsCD00022488        ATGTCACACCTCAGCCAGCAGAAAATTTACAGTGGGGAAAACCCCTTTGCCTGTAAGGTA
XM_005259214.1      ATGTCACACCTCAGCCAGCAGAGAATTTACAGTGGGGAAAACCCCTTTGCCTGTAAGGTA
NM_001099639.1      ATGTCACACCTCAGCCAGCAGAGAATTTACAGTGGGGAAAACCCCTTTGCCTGTAAGGTA
NM_001099638.1      ATGTCACACCTCAGCCAGCAGAGAATTTACAGTGGGGAAAACCCCTTTGCCTGTAAGGTA
NM_007145.2         ATGTCACACCTCAGCCAGCAGAGAATTTACAGTGGGGAAAACCCCTTTGCCTGTAAGGTA
                    **********************.*************************************

HsCD00022488        TGTGGAAAAGTCTTCAGCCACAAATCAAACCTCACTGAGCATGAGCATTTTCACACGAGA
XM_005259214.1      TGTGGAAAAGTCTTCAGCCACAAATCAAACCTCACTGAGCATGAGCATTTTCACACGAGA
NM_001099639.1      TGTGGAAAAGTCTTCAGCCACAAATCAAACCTCACTGAGCATGAGCATTTTCACACGAGA
NM_001099638.1      TGTGGAAAAGTCTTCAGCCACAAATCAAACCTCACTGAGCATGAGCATTTTCACACGAGA
NM_007145.2         TGTGGAAAAGTCTTCAGCCACAAATCAAACCTCACTGAGCATGAGCATTTTCACACGAGA
                    ************************************************************

HsCD00022488        GAAAACCTTTTG-AATGTAACGAGTGTGGAAAAGCCTTTAGCCAAAAGCAGTATGTCATT
XM_005259214.1      GAGAAACCTTTTGAATGTAACGAGTGTGGAAAAGCCTTTAGCCAAAAGCAGTATGTCATT
NM_001099639.1      GAGAAACCTTTTGAATGTAACGAGTGTGGAAAAGCCTTTAGCCAAAAGCAGTATGTCATT
NM_001099638.1      GAGAAACCTTTTGAATGTAACGAGTGTGGAAAAGCCTTTAGCCAAAAGCAGTATGTCATT
NM_007145.2         GAGAAACCTTTTGAATGTAACGAGTGTGGAAAAGCCTTTAGCCAAAAGCAGTATGTCATT
                    **.**.* ***  ***********************************************

HsCD00022488        AAACATCAGAACACCCATACTGGCGAGAAGCTTTTCGAATGTAATGAATGTGGAAAATCA
XM_005259214.1      AAACATCAGAACACCCATACTGGCGAGAAGCTTTTCGAATGTAATGAATGTGGAAAATCA
NM_001099639.1      AAACATCAGAACACCCATACTGGCGAGAAGCTTTTCGAATGTAATGAATGTGGAAAATCA
NM_001099638.1      AAACATCAGAACACCCATACTGGCGAGAAGCTTTTCGAATGTAATGAATGTGGAAAATCA
NM_007145.2         AAACATCAGAACACCCATACTGGCGAGAAGCTTTTCGAATGTAATGAATGTGGAAAATCA
                    ************************************************************

HsCD00022488        TTTAGCCAGAAGGAAAACCTCCTTACGCACCAGAAAATTCACACTGGAGAAAAACCTTTT
XM_005259214.1      TTTAGCCAGAAGGAAAACCTCCTTACGCACCAGAAAATTCACACTGGAGAAAAACCTTTT
NM_001099639.1      TTTAGCCAGAAGGAAAACCTCCTTACGCACCAGAAAATTCACACTGGAGAAAAACCTTTT
NM_001099638.1      TTTAGCCAGAAGGAAAACCTCCTTACGCACCAGAAAATTCACACTGGAGAAAAACCTTTT
NM_007145.2         TTTAGCCAGAAGGAAAACCTCCTTACGCACCAGAAAATTCACACTGGAGAAAAACCTTTT
                    ************************************************************

HsCD00022488        GAGTGTAAAGATTGCGGGAAAGCTTTCATTCAGAAGTCAAACCTCATCAGACACCAGAGA
XM_005259214.1      GAGTGTAAAGATTGCGGGAAAGCTTTCATTCAGAAGTCAAACCTCATCAGACACCAGAGA
NM_001099639.1      GAGTGTAAAGATTGCGGGAAAGCTTTCATTCAGAAGTCAAACCTCATCAGACACCAGAGA
NM_001099638.1      GAGTGTAAAGATTGCGGGAAAGCTTTCATTCAGAAGTCAAACCTCATCAGACACCAGAGA
NM_007145.2         GAGTGTAAAGATTGCGGGAAAGCTTTCATTCAGAAGTCAAACCTCATCAGACACCAGAGA
                    ************************************************************

HsCD00022488        ACTCACACAGGAGAGAAGCCCTTTGTATGTAAGGAGTGTGGAAAAACCTTCAGTGGCAAA
XM_005259214.1      ACTCACACAGGAGAGAAGCCCTTTGTATGTAAGGAGTGTGGAAAAACCTTCAGTGGCAAA
NM_001099639.1      ACTCACACAGGAGAGAAGCCCTTTGTATGTAAGGAGTGTGGAAAAACCTTCAGTGGCAAA
NM_001099638.1      ACTCACACAGGAGAGAAGCCCTTTGTATGTAAGGAGTGTGGAAAAACCTTCAGTGGCAAA
NM_007145.2         ACTCACACAGGAGAGAAGCCCTTTGTATGTAAGGAGTGTGGAAAAACCTTCAGTGGCAAA
                    ************************************************************

HsCD00022488        TCCAACCTTACTGAGCATGAGAAAATCCATATTGGAGAGAAGCCTTTTAAATGTAGTGAA
XM_005259214.1      TCCAACCTTACTGAGCATGAGAAAATCCATATTGGAGAGAAGCCTTTTAAATGTAGTGAA
NM_001099639.1      TCCAACCTTACTGAGCATGAGAAAATCCATATTGGAGAGAAGCCTTTTAAATGTAGTGAA
NM_001099638.1      TCCAACCTTACTGAGCATGAGAAAATCCATATTGGAGAGAAGCCTTTTAAATGTAGTGAA
NM_007145.2         TCCAACCTTACTGAGCATGAGAAAATCCATATTGGAGAGAAGCCTTTTAAATGTAGTGAA
                    ************************************************************

HsCD00022488        TGTGGAACAGCCTTTGGCCAGAAGAAGTACCTCATTG-----------------------
XM_005259214.1      TGTGGAACAGCCTTTGGCCAGAAGAAGTACCTCATAAAACATCAGAACATTCACACTGGA
NM_001099639.1      TGTGGAACAGCCTTTGGCCAGAAGAAGTACCTCATAAAACATCAGAACATTCACACTGGA
NM_001099638.1      TGTGGAACAGCCTTTGGCCAGAAGAAGTACCTCATAAAACATCAGAACATTCACACTGGA
NM_007145.2         TGTGGAACAGCCTTTGGCCAGAAGAAGTACCTCATAAAACATCAGAACATTCACACTGGA
                    ***********************************:.                       

HsCD00022488        ------------------------------------------------------------
XM_005259214.1      GAGAAACCCTATGAATGTAACGAATGTGGAAAAGCCTTCTCTCAGCGAACATCACTTATT
NM_001099639.1      GAGAAACCCTATGAATGTAACGAATGTGGAAAAGCCTTCTCTCAGCGAACATCACTTATT
NM_001099638.1      GAGAAACCCTATGAATGTAACGAATGTGGAAAAGCCTTCTCTCAGCGAACATCACTTATT
NM_007145.2         GAGAAACCCTATGAATGTAACGAATGTGGAAAAGCCTTCTCTCAGCGAACATCACTTATT
                                                                                

HsCD00022488        ------------------------------------------------------------
XM_005259214.1      GTACATGTGAGGATTCATTCAGGTGATAAACCTTACGAATGCAATGTTTGTGGAAAAGCC
NM_001099639.1      GTACATGTGAGGATTCATTCAGGTGATAAACCTTACGAATGCAATGTTTGTGGAAAAGCC
NM_001099638.1      GTACATGTGAGGATTCATTCAGGTGATAAACCTTACGAATGCAATGTTTGTGGAAAAGCC
NM_007145.2         GTACATGTGAGGATTCATTCAGGTGATAAACCTTACGAATGCAATGTTTGTGGAAAAGCC
                                                                                

HsCD00022488        ------------------------------------------------------------
XM_005259214.1      TTCTCTCAGAGCTCATCTCTCACTGTGCATGTGAGAAGCCATACAGGGGAGAAGCCCTAT
NM_001099639.1      TTCTCTCAGAGCTCATCTCTCACTGTGCATGTGAGAAGCCATACAGGGGAGAAGCCCTAT
NM_001099638.1      TTCTCTCAGAGCTCATCTCTCACTGTGCATGTGAGAAGCCATACAGGGGAGAAGCCCTAT
NM_007145.2         TTCTCTCAGAGCTCATCTCTCACTGTGCATGTGAGAAGCCATACAGGGGAGAAGCCCTAT
                                                                                

HsCD00022488        ------------------------------------------------------------
XM_005259214.1      GGTTGTAATGAATGTGGGAAAGCTTTCTCTCAGTTCTCAACCCTTGCTCTGCATTTGAGA
NM_001099639.1      GGTTGTAATGAATGTGGGAAAGCTTTCTCTCAGTTCTCAACCCTTGCTCTGCATTTGAGA
NM_001099638.1      GGTTGTAATGAATGTGGGAAAGCTTTCTCTCAGTTCTCAACCCTTGCTCTGCATTTGAGA
NM_007145.2         GGTTGTAATGAATGTGGGAAAGCTTTCTCTCAGTTCTCAACCCTTGCTCTGCATTTGAGA
                                                                                

HsCD00022488        ------------------------------------------------------------
XM_005259214.1      ATACACACAGGTAAGAAGCCTTATCAGTGCAGTGAATGTGGGAAAGCTTTCAGCCAGAAG
NM_001099639.1      ATACACACAGGTAAGAAGCCTTATCAGTGCAGTGAATGTGGGAAAGCTTTCAGCCAGAAG
NM_001099638.1      ATACACACAGGTAAGAAGCCTTATCAGTGCAGTGAATGTGGGAAAGCTTTCAGCCAGAAG
NM_007145.2         ATACACACAGGTAAGAAGCCTTATCAGTGCAGTGAATGTGGGAAAGCTTTCAGCCAGAAG
                                                                                

HsCD00022488        ---------------------------------------
XM_005259214.1      TCACACCACATTAGACACCAGAAAATTCATACTCACTAA
NM_001099639.1      TCACACCACATTAGACACCAGAAAATTCATACTCACTAA
NM_001099638.1      TCACACCACATTAGACACCAGAAAATTCATACTCACTAA
NM_007145.2         TCACACCACATTAGACACCAGAAAATTCATACTCACTAA
                                                           

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q15072
HPRD : 03296

Clone

Cloning Information : 25774
HIP Master Clone ID : 56150
Original Clone ID : FLH025774.01L

Organism


TAX_ID : 9606
Species Specific ID: 7705

Genome

Chromosome : 19
Map Location : 19q13.1
Ensembl : ENSG00000167635

Reagents

Labome : ZNF146-antibody