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ITGB8 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ITGB8
Gene Name:  integrin, beta 8
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH026771.01L
Keyword: potential short variant
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: Short variant or partial clone comparing to NM_002214
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00022526

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1320nts        
Open reading frame : 1 to 1320
Reference Sequence Alignment

ATGTGCGGCTCGGCCCTGGCTTTTTTTACCGCTGCATTTGTCTGCCTGCAAAACGACCGG
CGAGGTCCCGCCTCGTTCCTCTGGGCAGCCTGGGTGTTTTCACTTGTTCTTGGACTGGGC
CAAGGTGAAGACAATAGATGTGCATCTTCAAATGCAGCATCCTGTGCCAGGTGCCTTGCG
CTGGGTCCAGAATGTGGATGGTGTGTTCAAGAGGATTTCATTTCAGGTGGATCAAGAAGT
GAACGTTGTGATATTGTTTCCAATTTAATAAGCAAAGGCTGCTCAGTTGATTCAATAGAA
TACCCATCTGTGCATGTTATAATACCCACTGAAAATGAAATTAATACCCAGGTGACACCA
GGAGAAGTGTCTATCCAGCTGCGTCCAGGAGCCGAAGCTAATTTTATGCTGAAAGTTCAT
CCTCTGAAGAAATATCCTGTGGATCTTTATTATCTTGTTGATGTCTCAGCATCAATGCAC
AATAATATAGAAAAATTAAATTCCGTTGGAAACGATTTATCTAGAAAAATGGCATTTTTC
TCCCGTGACTTTCGTCTTGGATTTGGCTCATACGTTGATAAAACAGTTTCACCATACATT
AGCATCCACCCCGAAAGGATTCATAATCAATGCAGTGACTACAATTTAGACTGCATGCCT
CCCCATGGATACATCCATGTGCTGTCTTTGACAGAGAACATCACTGAGTTTGAGAAAGCA
GTTCATAGACAGAAGATCTCTGGAAACATAGATACACCAGAAGGAGGTTTTGACGCCATG
CTTCAGGCAGCTGTCTGTGAAAGTCATATCGGATGGCGAAAAGAGGCTAAAAGATTGCTG
CTGGTGATGACAGATCAGACGTCTCATCTCGCTCTTGATAGCAAATTGGCAGGCATAGTG
GTGCCCAATGACGGAAACTGTCATCTGAAAAACAACGTCTATGTCAAATCGACAACCATG
GAACACCCCTCACTAGGCCAACTTTCAGAGAAATTAATAGACAACAACATTAATGTCATC
TTTGCAGTTCAAGGAAAACAATTTCATTGGTATAAGGATCTTCTACCCCTCTTGCCAGGC
ACCATTGCTGGTGAAATAGAATCAAAGGCTGCAAACCTCAATAATTTGGTAGTGGAAGCC
TATCAGAAGCTCATTTCAGAAGTGAAAGTTCAGGTGGAAAACCAGGTACAAGGCATCTAT
TTTAACATTACCGCCATCTGTCCAGATGGGTCCAGAAAGCCAGGCATGGAAGGATGCAGA
AACGTGACGAGCAATGATGAAGTATGTGGGTGTGCATTTTTCCCTTTTAAAATAAACTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2034
Start on reference sequence 715
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 9 42
recombination site loxP site loxP recombination site 1015 1048
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ITGB8
Symbol Nomenclature : ITGB8
Designation : integrin beta-8
Full Nomenclature : integrin, beta 8
GENEID : 3696
HGNC : 6163
MIM : 604160
Vega : OTTHUMG00000023594
Target GenBank: BC002630

 

Reference Sequence Alignment

  

HsCD00022526      ATGTGCGGCTCGGCCCTGGCTTTTTTTACCGCTGCATTTGTCTGCCTGCAAAACGACCGG
NM_002214.2       ATGTGCGGCTCGGCCCTGGCTTTTTTTACCGCTGCATTTGTCTGCCTGCAAAACGACCGG
                  ************************************************************

HsCD00022526      CGAGGTCCCGCCTCGTTCCTCTGGGCAGCCTGGGTGTTTTCACTTGTTCTTGGACTGGGC
NM_002214.2       CGAGGTCCCGCCTCGTTCCTCTGGGCAGCCTGGGTGTTTTCACTTGTTCTTGGACTGGGC
                  ************************************************************

HsCD00022526      CAAGGTGAAGACAATAGATGTGCATCTTCAAATGCAGCATCCTGTGCCAGGTGCCTTGCG
NM_002214.2       CAAGGTGAAGACAATAGATGTGCATCTTCAAATGCAGCATCCTGTGCCAGGTGCCTTGCG
                  ************************************************************

HsCD00022526      CTGGGTCCAGAATGTGGATGGTGTGTTCAAGAGGATTTCATTTCAGGTGGATCAAGAAGT
NM_002214.2       CTGGGTCCAGAATGTGGATGGTGTGTTCAAGAGGATTTCATTTCAGGTGGATCAAGAAGT
                  ************************************************************

HsCD00022526      GAACGTTGTGATATTGTTTCCAATTTAATAAGCAAAGGCTGCTCAGTTGATTCAATAGAA
NM_002214.2       GAACGTTGTGATATTGTTTCCAATTTAATAAGCAAAGGCTGCTCAGTTGATTCAATAGAA
                  ************************************************************

HsCD00022526      TACCCATCTGTGCATGTTATAATACCCACTGAAAATGAAATTAATACCCAGGTGACACCA
NM_002214.2       TACCCATCTGTGCATGTTATAATACCCACTGAAAATGAAATTAATACCCAGGTGACACCA
                  ************************************************************

HsCD00022526      GGAGAAGTGTCTATCCAGCTGCGTCCAGGAGCCGAAGCTAATTTTATGCTGAAAGTTCAT
NM_002214.2       GGAGAAGTGTCTATCCAGCTGCGTCCAGGAGCCGAAGCTAATTTTATGCTGAAAGTTCAT
                  ************************************************************

HsCD00022526      CCTCTGAAGAAATATCCTGTGGATCTTTATTATCTTGTTGATGTCTCAGCATCAATGCAC
NM_002214.2       CCTCTGAAGAAATATCCTGTGGATCTTTATTATCTTGTTGATGTCTCAGCATCAATGCAC
                  ************************************************************

HsCD00022526      AATAATATAGAAAAATTAAATTCCGTTGGAAACGATTTATCTAGAAAAATGGCATTTTTC
NM_002214.2       AATAATATAGAAAAATTAAATTCCGTTGGAAACGATTTATCTAGAAAAATGGCATTTTTC
                  ************************************************************

HsCD00022526      TCCCGTGACTTTCGTCTTGGATTTGGCTCATACGTTGATAAAACAGTTTCACCATACATT
NM_002214.2       TCCCGTGACTTTCGTCTTGGATTTGGCTCATACGTTGATAAAACAGTTTCACCATACATT
                  ************************************************************

HsCD00022526      AGCATCCACCCCGAAAGGATTCATAATCAATGCAGTGACTACAATTTAGACTGCATGCCT
NM_002214.2       AGCATCCACCCCGAAAGGATTCATAATCAATGCAGTGACTACAATTTAGACTGCATGCCT
                  ************************************************************

HsCD00022526      CCCCATGGATACATCCATGTGCTGTCTTTGACAGAGAACATCACTGAGTTTGAGAAAGCA
NM_002214.2       CCCCATGGATACATCCATGTGCTGTCTTTGACAGAGAACATCACTGAGTTTGAGAAAGCA
                  ************************************************************

HsCD00022526      GTTCATAGACAGAAGATCTCTGGAAACATAGATACACCAGAAGGAGGTTTTGACGCCATG
NM_002214.2       GTTCATAGACAGAAGATCTCTGGAAACATAGATACACCAGAAGGAGGTTTTGACGCCATG
                  ************************************************************

HsCD00022526      CTTCAGGCAGCTGTCTGTGAAAGTCATATCGGATGGCGAAAAGAGGCTAAAAGATTGCTG
NM_002214.2       CTTCAGGCAGCTGTCTGTGAAAGTCATATCGGATGGCGAAAAGAGGCTAAAAGATTGCTG
                  ************************************************************

HsCD00022526      CTGGTGATGACAGATCAGACGTCTCATCTCGCTCTTGATAGCAAATTGGCAGGCATAGTG
NM_002214.2       CTGGTGATGACAGATCAGACGTCTCATCTCGCTCTTGATAGCAAATTGGCAGGCATAGTG
                  ************************************************************

HsCD00022526      GTGCCCAATGACGGAAACTGTCATCTGAAAAACAACGTCTATGTCAAATCGACAACCATG
NM_002214.2       GTGCCCAATGACGGAAACTGTCATCTGAAAAACAACGTCTATGTCAAATCGACAACCATG
                  ************************************************************

HsCD00022526      GAACACCCCTCACTAGGCCAACTTTCAGAGAAATTAATAGACAACAACATTAATGTCATC
NM_002214.2       GAACACCCCTCACTAGGCCAACTTTCAGAGAAATTAATAGACAACAACATTAATGTCATC
                  ************************************************************

HsCD00022526      TTTGCAGTTCAAGGAAAACAATTTCATTGGTATAAGGATCTTCTACCCCTCTTGCCAGGC
NM_002214.2       TTTGCAGTTCAAGGAAAACAATTTCATTGGTATAAGGATCTTCTACCCCTCTTGCCAGGC
                  ************************************************************

HsCD00022526      ACCATTGCTGGTGAAATAGAATCAAAGGCTGCAAACCTCAATAATTTGGTAGTGGAAGCC
NM_002214.2       ACCATTGCTGGTGAAATAGAATCAAAGGCTGCAAACCTCAATAATTTGGTAGTGGAAGCC
                  ************************************************************

HsCD00022526      TATCAGAAGCTCATTTCAGAAGTGAAAGTTCAGGTGGAAAACCAGGTACAAGGCATCTAT
NM_002214.2       TATCAGAAGCTCATTTCAGAAGTGAAAGTTCAGGTGGAAAACCAGGTACAAGGCATCTAT
                  ************************************************************

HsCD00022526      TTTAACATTACCGCCATCTGTCCAGATGGGTCCAGAAAGCCAGGCATGGAAGGATGCAGA
NM_002214.2       TTTAACATTACCGCCATCTGTCCAGATGGGTCCAGAAAGCCAGGCATGGAAGGATGCAGA
                  ************************************************************

HsCD00022526      AACGTGACGAGCAATGATGAAGTATGTGGGTGTGCATTTTTCCCTTTTAAAATAAACTTG
NM_002214.2       AACGTGACGAGCAATGATGAAGTTCTTTTCAATGTAACAGTTACAATGAAAAAATGTGAT
                  ***********************:  *   :.** *: : * .*::* ****:*:.  : 

HsCD00022526      ------------------------------------------------------------
NM_002214.2       GTCACAGGAGGAAAAAACTATGCAATAATCAAACCTATTGGTTTTAATGAAACCGCTAAA
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       ATTCATATACACAGAAACTGCAGCTGTCAGTGTGAGGACAACAGAGGACCTAAAGGAAAG
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       TGTGTAGATGAAACTTTTCTAGATTCCAAGTGTTTCCAGTGTGATGAGAATAAATGTCAT
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       TTTGATGAAGATCAGTTTTCTTCTGAGAGTTGCAAGTCACACAAGGATCAGCCTGTTTGC
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       AGTGGTCGAGGAGTTTGTGTTTGTGGGAAATGTTCATGTCACAAAATTAAGCTTGGAAAA
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       GTGTATGGAAAATACTGTGAAAAGGATGACTTTTCTTGTCCATATCACCATGGAAATCTG
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       TGTGCTGGGCATGGAGAGTGTGAAGCAGGCAGATGCCAATGCTTCAGTGGCTGGGAAGGT
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       GATCGATGCCAGTGCCCTTCAGCAGCAGCCCAGCACTGTGTCAATTCAAAGGGCCAAGTG
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       TGCAGTGGAAGAGGCACGTGTGTGTGTGGAAGGTGTGAGTGCACCGATCCCAGGAGCATC
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       GGCCGCTTCTGTGAACACTGCCCCACCTGTTATACAGCCTGCAAGGAAAACTGGAATTGT
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       ATGCAATGCCTTCACCCTCACAATTTGTCTCAGGCTATACTTGATCAGTGCAAAACCTCA
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       TGTGCTCTCATGGAACAACAGCATTATGTCGACCAAACTTCAGAATGTTTCTCCAGCCCA
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       AGCTACTTGAGAATATTTTTCATCATTTTCATAGTTACATTCTTGATTGGGTTGCTTAAA
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       GTCCTGATCATTAGACAGGTGATACTACAATGGAATAGTAATAAAATTAAGTCCTCATCA
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       GATTACAGAGTGTCAGCCTCAAAAAAGGATAAGTTGATTCTGCAAAGTGTTTGCACAAGA
                                                                              

HsCD00022526      ------------------------------------------------------------
NM_002214.2       GCAGTCACCTACCGACGTGAGAAGCCTGAAGAAATAAAAATGGATATCAGCAAATTAAAT
                                                                              

HsCD00022526      ------------------------------
NM_002214.2       GCTCATGAAACTTTCAGGTGCAACTTCTAA
                                                

Pathways

NCI : Beta5 beta6 beta7 and beta8 integrin cell surface interactions
NCI : Integrin family cell surface interactions
Panther : Integrin signalling pathway
Reactome : Elastic fibre formation
Reactome : Extracellular matrix organization
Reactome : Integrin cell surface interactions
Reactome : Molecules associated with elastic fibres
WikiPathway : Focal Adhesion
WikiPathway : Integrin-mediated cell adhesion

Clone

Cloning Information : 26771
HIP Master Clone ID : 57147
Original Clone ID : FLH026771.01L

Organism


TAX_ID : 9606
Species Specific ID: 3696

Genome

Chromosome : 7
Map Location : 7p21.1
Ensembl : ENSG00000105855

Reagents

Labome : ITGB8-antibody