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TAZ (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TAZ
Gene Name:  tafazzin
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH028594.01X
Keyword: potential short variant
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: Short variant or partial clone comparing to NM_000116
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00022543

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 789nts         Open reading frame : 1 to 789

ATGCCTCTGCACGTGAAGTGGCCGTTCCCCGCGGTGCCGCCGCTCACCTGGACCCTGGCC
AGCAGCGTCGTCATGGGCTTGGTGGGCACCTACAGCTGCTTCTGGACCAGTGAGTGGGCC
CAGGCCGAGGCAGGCCCGCCCGGGTACCCATGCCCGGCCGGAGGGATCCTGAAACTCCGC
CACATCTGGAACCTGAAGTTGATGCGTTGGACCCCTGCAGCTGCAGACATCTGCTTCACC
AAGGAGCTACACTCCCACTTCTTCAGCTTGGGCAAGTGTGTGCCTGTGTGCCGAGGAGAT
GGCGTCTACCAGAAGGGGATGGACTTCATTTTGGAGAAGCTCAACCATGGGGACTGGGTG
CATATCTTCCCAGAAGGAATCGGGCGCCTGATTGCTGAGTGTCATCTCAACCCCATCATC
CTGCCCCTGTGGCATGTCGGTGAGCCTGGGGACGGGGACAGAGAGATGGCATCTGGGGTG
GGGGGCCTGGGACTCCCTCTGGTCCCAGGCTGCCCTGCTCCACCCCACGTCTGGCCTTCT
GTCCACTGTGCTGCAGGAATGAATGACGTCCTTCCTAACAGTCCGCCCTACTTCCCCCGC
TTTGGACAGAAAATCACTGTGCTGATCGGGAAGCCCTTCAGTGCCCTGCCTGTACTCGAG
CGGCTCCGGGCGGAGAACAAGTCGGCTGTGGAGATGCGGAAAGCCCTGACGGACTTCATT
CAAGAGGAATTCCAGCATCTGAAGACTCAGGCAGAGCAGCTCCACAACCACCTCCAGCCT
GGGAGATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 886
Start on reference sequence 98
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TAZ
Symbol Nomenclature : TAZ
SYNONYM : BTHS; CMD3A; EFE; EFE2; G4.5; LVNCX; Taz1
Designation : protein G4.5
Full Nomenclature : tafazzin
GENEID : 6901
Locus Tag : XX-FW83563B9.3
GI : 60654760
GenBank Accession : AY889154
HGNC : 11577
MIM : 300394
Vega : OTTHUMG00000033190
Target GenBank: BC011515

 

Pathways

Reactome : Acyl chain remodeling of CL
Reactome : Glycerophospholipid biosynthesis
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Metabolism of proteins
Reactome : Mitochondrial protein import
Reactome : Phospholipid metabolism

Protein

SMART domain : PlsC : Phosphate acyltransferases
UniProt : Q16635
HPRD : 02316

Clone

Cloning Information : 28594
HIP Master Clone ID : 58970
Original Clone ID : FLH028594.01X

Organism


TAX_ID : 9606
Species Specific ID: 6901

Genome

Chromosome : X
Map Location : Xq28
Ensembl : ENSG00000102125

Reagents

Labome : TAZ-antibody