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Detailed Vector Information: pANT7_nHA

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: pANT7_nHA
Type: No insert
Vector Name: pANT7_nHA              
Source: HIP
Description: In vitro expression vector, makes N-term HA fusion; amp resistance; recombinational cloning.
Comments: Constructed at HIP for expression via T7 coupled Rabbit retic lysate for NAPPA protein arrays; note difference growth, host for empty vector versus with insert forms
Publications: PMID: 15232106
Title: Self-assembling protein microarrays
Authors: Al Lau
Niroshan Ramachandran
Joshua LaBaer
EvNO00023110

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Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DB3.1
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pANT7_nHA

pANT7_nHA in advanced viewed

Synonyms: pANT7-nHA
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: In vitro expression vector, makes N-term HA fusion; amp resistance; recombinational cloning.
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays at HIP
Size (bp): 5339
Parent Vector: None
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 2624 3306
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1894 2103
promoter T7 T7 transcriptional start sequence 5324 5339
recombination site attR1 AttR1 site for recombination in empty vector 567 583
recombination site attR2 AttR2 site for recombination in empty vector 2254 2269
selectable marker AmpR ampicillin resistance gene 3404 4063
selectable marker CmR Chloramphenicol resistance gene 800 1459
ssDNA origin f1 F1 origin for ssDNA production 4832 5138
tag HA HA epitope tag 531 557
trxn termination sequence T7 term T7 terminator 2396 2442

 

Original Clone ID: pANT7_nHA
Species Specific ID: None
Target GenBank: None