Gene:
Gene Symbol:
No insert
Gene Name:
No insert
Original Clone ID:
pANT7_nHA
Type:
No insert
Vector Name:
pANT7_nHA
Source:
HIP
Description:
In vitro expression vector, makes N-term HA fusion; amp resistance; recombinational cloning.
Comments:
Constructed at HIP for expression via T7 coupled Rabbit retic lysate for NAPPA protein arrays; note difference growth, host for empty vector versus with insert forms
Publications:
Authors:
Joshua LaBaer
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Al Lau
EvNO00023110
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Recommended Growth Condition:
Distributed in bacterial strain:
DB3.1
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Vector Name:
pANT7_nHA
Synonyms:
pANT7-nHA
Description:
In vitro expression vector, makes N-term HA fusion; amp resistance; recombinational cloning.
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays at HIP
Size (bp):
5339
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
2624
3306
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1894
2103
promoter
T7
T7 transcriptional start sequence
5324
5339
recombination site
attR1
AttR1 site for recombination in empty vector
567
583
recombination site
attR2
AttR2 site for recombination in empty vector
2254
2269
selectable marker
CmR
Chloramphenicol resistance gene
800
1459
selectable marker
AmpR
ampicillin resistance gene
3404
4063
ssDNA origin
f1
F1 origin for ssDNA production
4832
5138
tag
HA
HA epitope tag
531
557
trxn termination sequence
T7 term
T7 terminator
2396
2442
