DNASU Plasmid Repository • 480.965.2857 | Email

Detailed Vector Information: pCS105

TOP

Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: p079
Type: No insert
Vector Name: pCS105              
Source: Ramesh Shivdasani
Description: Mammalian expression vector with CMV promoter, T3, SP6; ampicillin resistance; restriction enzyme cloning (two MCSs).
Comments: Targeting vector with neo flanked by 2 loxP; from pPNT3.LoxP5'; has neomycin cassette flanked with two LoxP oriented in same direction
Publications: PMID: 9660951
Title: The Xenopus dorsalizing factor Gremlin identifies a novel family of secreted proteins that antagonize BMP activities
PMID: 11369202
Title: A lineage-restricted and divergent beta-tubulin isoform is essential for the biogenesis, structure and function of blood platelets
Authors: P Lecine
Ramesh Shivdasani
Robbyn Issner
EvNO00023137

Price:  Login for Pricing
NOT AVAILABLE
CPD only
Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : T1 Phage-resistant cloning strain
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pCS105

pCS105 in advanced viewed

Synonyms: pCS, pCS2, pDH105
Description: Mammalian expression vector with CMV promoter, T3, SP6; ampicillin resistance; restriction enzyme cloning (two MCSs).
Comments: Vector author notes T7 defective, use T3 to make antisense
Size (bp): 4100
Parent Vector: None
Properties: in vitro transcription, mammalian expression, multiple cloning site, ssDNA production
Author Name: David Hsu
RM Harland
Publications: PMID: 9660951
Title: The Xenopus dorsalizing factor Gremlin identifies a novel family of secreted proteins that antagonize BMP activities

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS1 MCS 1 (including SalI, SfiI, NotI, EagI, others) 0 0
MCS MCS2 MCS 2 (including AscI, KpnI) 0 0
bacterial origin ColE1 ori ColE1 bacterial origin 0 0
promoter CMV sCMVIE94 promoter 0 0
promoter SP6 SP6 promoter 0 0
promoter T7 T7 promoter (probably defective) 0 0
promoter T3 T3 promoter for in vitro trxn 0 0
selectable marker AmpR ampicillan resistance gnee 0 0
ssDNA origin F1 F1 origin 0 0

 

Original Clone ID: p079
SYNONYM: pPNT6
SYNONYM: pPNT6 (verified 2004)
Species Specific ID: None
Target GenBank: None