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BMH2 (Saccharomyces cerevisiae) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  BMH2
Gene Name:  14-3-3 protein, minor isoform; binds proteins and DNA, involved in regulation of many processes including exocytosis and vesicle transport, Ras/MAPK signaling during pseudohyphal development, rapamycin-sensitive signaling, and others
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH001624.01X
Keyword: None
Species: Saccharomyces cerevisiae
Type: cDNA
Vector Name: pDONR201               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
ScCD00023397

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 822nts         Open reading frame : 1 to 822

ATGTCCCAAACTCGTGAAGATTCTGTTTACCTAGCTAAATTAGCTGAACAAGCCGAACGT
TATGAAGAAATGGTCGAAAACATGAAGGCCGTTGCTTCATCAGGTCAAGAGTTATCTGTC
GAAGAACGGAATCTATTGTCGGTTGCTTACAAGAACGTCATCGGTGCTCGCCGTGCTTCA
TGGAGAATAGTTTCTTCGATCGAACAAAAAGAAGAATCAAAGGAGAAATCTGAACATCAA
GTTGAATTAATCCGTTCTTACCGTTCTAAAATTGAAACTGAATTGACCAAAATCTCTGAC
GACATTTTATCTGTGTTAGATTCTCATTTAATCCCTTCTGCTACTACTGGTGAGTCTAAA
GTATTTTACTATAAGATGAAGGGTGACTACCACCGTTATTTAGCTGAATTTTCCAGCGGA
GATGCAAGAGAAAAGGCAACCAACTCCTCTTTGGAGGCTTATAAAACCGCTTCCGAAATC
GCCACAACTGAATTGCCTCCAACTCACCCAATTCGTTTAGGTCTAGCTTTGAATTTCTCC
GTCTTCTATTACGAAATTCAAAACTCTCCTGATAAGGCTTGCCACTTGGCCAAACAAGCC
TTTGATGATGCTATTGCTGAGTTAGATACTTTATCTGAAGAATCATACAAGGATAGCACT
TTGATCATGCAATTATTAAGGGACAACTTGACCTTATGGACCTCTGATATTTCTGAATCT
GGTCAAGAAGATCAACAACAACAACAACAACAGCAACAGCAACAGCAACAACAGCAACAA
CAAGCTCCAGCTGAACAAACTCAAGGTGAACCAACCAAATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 822
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p TCCAGCTGACCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)CATGGCAATTCCCGGGGAT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Protein
  • Clone
  • Organism

Gene

Target GenBank: CAA59275

 

Protein

SMART domain : 14_3_3 : 14-3-3 homologues

Clone

Cloning Information : 1624
HIP Master Clone ID : 1182
Original Clone ID : FLH001624.01X

Organism


Species Specific ID: YDR099W