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REQ (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  DPF2
Gene Name:  D4, zinc and double PHD fingers family 2
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH110500.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00002343

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1176nts         Open reading frame : 1 to 1176
Reference Sequence Alignment

ATGGCGGCTGTGGTGGAGAATGTAGTGAAGCTCCTTGGGGAGCAGTACTACAAAGATGCC
ATGGAGCAGTGCCACAATTACAATGCTCGCCTCTGTGCTGAGCGCAGCGTGCGCCTGCCT
TTCTTGGACTCACAGACCGGAGTAGCCCAGAGCAATTGTTACATCTGGATGGAAAAGCGA
CACCGGGGTCCAGGATTGGCCTCCGGACAGCTGTACTCCTACCCTGCCCGGCGCTGGCGG
AAAAAGCGGCGAGCCCATCCCCCTGAGGATCCACGACTTTCCTTCCCATCTATTAAGCCA
GACACAGACCAGACCCTGAAGAAGGAGGGGCTGATCTCTCAGGATGGCAGTAGTTTAGAG
GCTCTGTTGCGCACTGACCCCCTGGAGAAGCGAGGTGCCCCGGATCCCCGAGTTGATGAT
GACAGCCTGGGCGAGTTTCCTGTGACCAACAGTCGAGCGCGAAAGCGGATCCTAGAACCA
GATGACTTCCTGGATGACCTCGATGATGAAGACTATGAAGAAGATACTCCCAAGCGTCGG
GGAAAGGGGAAATCCAAGGGTAAGGGTGTGGGCAGTGCCCGTAAGAAGCTGGATGCTTCC
ATCCTGGAGGACCGGGATAAGCCCTATGCCTGTGACATTTGTGGAAAACGTTACAAGAAC
CGACCAGGCCTCAGTTACCACTATGCCCACTCCCACTTGGCTGAGGAGGAGGGCGAGGAC
AAGGAAGACTCTCAACCACCCACTCCTGTTTCCCAGAGGTCTGAGGAGCAGAAATCCAAA
AAGGGTCCTGATGGATTGGCCTTGCCCAACAACTACTGTGACTTCTGCCTGGGGGACTCA
AAGATTAACAAGAAGACGGGACAACCCGAGGAGCTGGTGTCCTGTTCTGACTGTGGCCGC
TCAGGGCATCCATCTTGCCTCCAATTTACCCCCGTGATGATGGCGGCAGTGAAGACATAC
CGCTGGCAGTGCATCGAGTGCAAATGTTGCAATATCTGCGGCACCTCCGAGAATGACGAC
CAGTTGCTCTTCTGTGATGACTGCGATCGTGGCTACCACATGTACTGTCTCACCCCGTCC
ATGTCTGAGCCCCCTGAAGGAAGTTGGAGCTGCCACCTGTGTCTGGACCTGTTGAAAGAG
AAAGCTTCCATCTACCAGAACCAGAACTCCTCTTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1199
Start on reference sequence 24
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DPF2
Symbol Nomenclature : DPF2
SYNONYM : REQ; UBID4; ubi-d4
Designation : BAF45D|BRG1-associated factor 45D|apoptosis response zinc finger protein|protein requiem|requiem, apoptosis response zinc finger|zinc finger protein ubi-d4
Full Nomenclature : D4, zinc and double PHD fingers family 2
GENEID : 5977
GI : 61361063
GenBank Accession : AY889041
HGNC : 9964
MIM : 601671
Vega : OTTHUMG00000165985
Target GenBank: BC014889

 

Reference Sequence Alignment

  

HsCD00002343        ATGGCGGCTGTGGTGGAGAATGTAGTGAAGCTCCTTGGGGAGCAGTACTACAAAGATGCC
XM_005274149.1      ATGGCGGCTGTGGTGGAGAATGTAGTGAAGCTCCTTGGGGAGCAGTACTACAAAGATGCC
NM_006268.4         ATGGCGGCTGTGGTGGAGAATGTAGTGAAGCTCCTTGGGGAGCAGTACTACAAAGATGCC
                    ************************************************************

HsCD00002343        ATGGAGCAGTGCCACAATTACAATGCTCGCCTCTGTGCTGAGCGCAGCGTGCGCCTGCCT
XM_005274149.1      ATGGAGCAGTGCCACAATTACAATGCTCGCCTCTGTGCTGAGCGCAGCGTGCGCCTGCCT
NM_006268.4         ATGGAGCAGTGCCACAATTACAATGCTCGCCTCTGTGCTGAGCGCAGCGTGCGCCTGCCT
                    ************************************************************

HsCD00002343        TTCTTGGACTCACAGACCGGAGTAGCCCAGAGCAATTGTTACATCTGGATGGAAAAGCGA
XM_005274149.1      TTCTTGGACTCACAGACCGGAGTAGCCCAGAGCAATTGTTACATCTGGATGGAAAAGCGA
NM_006268.4         TTCTTGGACTCACAGACCGGAGTAGCCCAGAGCAATTGTTACATCTGGATGGAAAAGCGA
                    ************************************************************

HsCD00002343        CACCGGGGTCCAGGATTGGCCTCCGGACAGCTGTACTCCTACCCTGCCCGGCGCTGGCGG
XM_005274149.1      CACCGGGGTCCAGGATTGGCCTCCGGACAGCTGTACTCCTACCCTGCCCGGCGCTGGCGG
NM_006268.4         CACCGGGGTCCAGGATTGGCCTCCGGACAGCTGTACTCCTACCCTGCCCGGCGCTGGCGG
                    ************************************************************

HsCD00002343        AAAAAGCGGCGAGCCCATCCCCCTGAGGATCCACGACTTTCCTTCCCATCTATTAAGCCA
XM_005274149.1      AAAAAGCGGCGAGCCCATCCCCCTGAGGATCCACGACTTTCCTTCCCATCTATTAAGCCA
NM_006268.4         AAAAAGCGGCGAGCCCATCCCCCTGAGGATCCACGACTTTCCTTCCCATCTATTAAGCCA
                    ************************************************************

HsCD00002343        GACACAGACCAGACCCTGAAGAAGGAGGGGCTGATCTCTCAGGATGGCAGTAGTTTAGAG
XM_005274149.1      GACACAGACCAGACCCTGAAGAAGGAGGGGCTGATCTCTCAGGATGGCAGTAGTTTAGAG
NM_006268.4         GACACAGACCAGACCCTGAAGAAGGAGGGGCTGATCTCTCAGGATGGCAGTAGTTTAGAG
                    ************************************************************

HsCD00002343        GCTCTGTTGCGCACTGACCCCCTGGAGAAGCGAGGTGCCCCGGATCCCCGAGTTGATGAT
XM_005274149.1      GCTCTGTTGCGCACTGACCCCCTGGAGAAGCGAGGTGCCCCGGATCCCCGAGTTGATGAT
NM_006268.4         GCTCTGTTGCGCACTGACCCCCTGGAGAAGCGAGGTGCCCCGGATCCCCGAGTTGATGAT
                    ************************************************************

HsCD00002343        GACAGCCTGGGCGAGTTTCCTGTGACCAACAGTCGAGCGCGAAAGCGGATCCTAGAACCA
XM_005274149.1      GACAGCCTGGGCGAGTTTCCTGTGACCAACAGTCGAGCGCGAAAGCGGATCCTAGAACCA
NM_006268.4         GACAGCCTGGGCGAGTTTCCTGTGACCAACAGTCGAGCGCGAAAGCGGATCCTAGAACCA
                    ************************************************************

HsCD00002343        GATGACTTCCTGGATGACCTCGATGATGAAGACTATGAAGAAGATACTCCCAAGCGTCGG
XM_005274149.1      GATGACTTCCTGGATGACCTCGATGATGAAGACTATGAAGAAGATACTCCCAAGCGTCGG
NM_006268.4         GATGACTTCCTGGATGACCTCGATGATGAAGACTATGAAGAAGATACTCCCAAGCGTCGG
                    ************************************************************

HsCD00002343        GGAAAGGGGAAATCCAAGGGTAAGGGTGTGGGCAGTGCCCGTAAGAAGCTGGATGCTTCC
XM_005274149.1      GGAAAGGGGAAATCCAAGGGTAAGGGTGTGGGCAGTGCCCGTAAGAAGCTGGATGCTTCC
NM_006268.4         GGAAAGGGGAAATCCAAGGGTAAGGGTGTGGGCAGTGCCCGTAAGAAGCTGGATGCTTCC
                    ************************************************************

HsCD00002343        ATCCTGGAGGACCGGGATAAGCCCTATGCCTGTGACATTTG-------------------
XM_005274149.1      ATCCTGGAGGACCGGGATAAGCCCTATGCCTGTGACAATAGTTTCAAACAAAAGCATACC
NM_006268.4         ATCCTGGAGGACCGGGATAAGCCCTATGCCTGTGACATTTG-------------------
                    *************************************:*:*                   

HsCD00002343        -----------------------TGGAAAACGTTACAAGAACCGACCAGGCCTCAGTTAC
XM_005274149.1      TCGAAAGCGCCCCAGAGAGTTTGTGGAAAACGTTACAAGAACCGACCAGGCCTCAGTTAC
NM_006268.4         -----------------------TGGAAAACGTTACAAGAACCGACCAGGCCTCAGTTAC
                                           *************************************

HsCD00002343        CACTATGCCCACTCCCACTTGGCTGAGGAGGAGGGCGAGGACAAGGAAGACTCTCAACCA
XM_005274149.1      CACTATGCCCACTCCCACTTGGCTGAGGAGGAGGGCGAGGACAAGGAAGACTCTCAACCA
NM_006268.4         CACTATGCCCACTCCCACTTGGCTGAGGAGGAGGGCGAGGACAAGGAAGACTCTCAACCA
                    ************************************************************

HsCD00002343        CCCACTCCTGTTTCCCAGAGGTCTGAGGAGCAGAAATCCAAAAAGGGTCCTGATGGATTG
XM_005274149.1      CCCACTCCTGTTTCCCAGAGGTCTGAGGAGCAGAAATCCAAAAAGGGTCCTGATGGATTG
NM_006268.4         CCCACTCCTGTTTCCCAGAGGTCTGAGGAGCAGAAATCCAAAAAGGGTCCTGATGGATTG
                    ************************************************************

HsCD00002343        GCCTTGCCCAACAACTACTGTGACTTCTGCCTGGGGGACTCAAAGATTAACAAGAAGACG
XM_005274149.1      GCCTTGCCCAACAACTACTGTGACTTCTGCCTGGGGGACTCAAAGATTAACAAGAAGACG
NM_006268.4         GCCTTGCCCAACAACTACTGTGACTTCTGCCTGGGGGACTCAAAGATTAACAAGAAGACG
                    ************************************************************

HsCD00002343        GGACAACCCGAGGAGCTGGTGTCCTGTTCTGACTGTGGCCGCTCAGGGCATCCATCTTGC
XM_005274149.1      GGACAACCCGAGGAGCTGGTGTCCTGTTCTGACTGTGGCCGCTCAGGGCATCCATCTTGC
NM_006268.4         GGACAACCCGAGGAGCTGGTGTCCTGTTCTGACTGTGGCCGCTCAGGGCATCCATCTTGC
                    ************************************************************

HsCD00002343        CTCCAATTTACCCCCGTGATGATGGCGGCAGTGAAGACATACCGCTGGCAGTGCATCGAG
XM_005274149.1      CTCCAATTTACCCCCGTGATGATGGCGGCAGTGAAGACATACCGCTGGCAGTGCATCGAG
NM_006268.4         CTCCAATTTACCCCCGTGATGATGGCGGCAGTGAAGACATACCGCTGGCAGTGCATCGAG
                    ************************************************************

HsCD00002343        TGCAAATGTTGCAATATCTGCGGCACCTCCGAGAATGACGACCAGTTGCTCTTCTGTGAT
XM_005274149.1      TGCAAATGTTGCAATATCTGCGGCACCTCCGAGAATGACGACCAGTTGCTCTTCTGTGAT
NM_006268.4         TGCAAATGTTGCAATATCTGCGGCACCTCCGAGAATGACGACCAGTTGCTCTTCTGTGAT
                    ************************************************************

HsCD00002343        GACTGCGATCGTGGCTACCACATGTACTGTCTCACCCCGTCCATGTCTGAGCCCCCTGAA
XM_005274149.1      GACTGCGATCGTGGCTACCACATGTACTGTCTCACCCCGTCCATGTCTGAGCCCCCTGAA
NM_006268.4         GACTGCGATCGTGGCTACCACATGTACTGTCTCACCCCGTCCATGTCTGAGCCCCCTGAA
                    ************************************************************

HsCD00002343        GGAAGTTGGAGCTGCCACCTGTGTCTGGACCTGTTGAAAGAGAAAGCTTCCATCTACCAG
XM_005274149.1      GGAAGTTGGAGCTGCCACCTGTGTCTGGACCTGTTGAAAGAGAAAGCTTCCATCTACCAG
NM_006268.4         GGAAGTTGGAGCTGCCACCTGTGTCTGGACCTGTTGAAAGAGAAAGCTTCCATCTACCAG
                    ************************************************************

HsCD00002343        AACCAGAACTCCTCTTAG
XM_005274149.1      AACCAGAACTCCTCTTGA
NM_006268.4         AACCAGAACTCCTCTTGA
                    ****************..

Protein

SMART domain : PHD : PHD zinc finger
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q92785
HPRD : 03394

Clone

Cloning Information : 110500
HIP Master Clone ID : 86585
Original Clone ID : FLH110500.01X

Organism


TAX_ID : 9606
Species Specific ID: 5977

Genome

Chromosome : 11
Map Location : 11q13
Ensembl : ENSG00000133884

Reagents

Labome : DPF2-antibody