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ARG5,6 (Saccharomyces cerevisiae) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ARG5,6
Gene Name:  Protein that is processed in the mitochondrion to yield acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase, which catalyze the 2nd and 3rd steps in arginine biosynthesis; enzymes form a complex with Arg2p
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH112771.01X
Keyword: None
Species: Saccharomyces cerevisiae
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
ScCD00023716

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2592nts         Open reading frame : 1 to 2592

ATGCCATCTGCTAGCTTACTCGTCTCGACAAAGAGACTTAACGCTTCCAAATTCCAAAAA
TTTGTGTCTTCATTAAACAAATCCACCATAGCAGGATTTGCATCTGTACCCTTGAGAGCT
CCACCATCCGTTGCATTTACGAGAAAGAAAGTCGGATACTCAAAGAGGTATGTTTCATCT
ACTAACGGCTTTTCAGCTACTAGATCCACTGTGATCCAACTGTTGAACAATATCAGCACA
AAAAGAGAGGTTGAACAATATTTGAAATATTTCACTTCCGTCTCACAACAACAATTTGCT
GTGATCAAGGTGGGTGGTGCCATTATCAGCGACAATCTACACGAACTCGCTTCCTGCTTG
GCATTTTTGTATCATGTTGGTCTATATCCAATAGTTTTACATGGTACCGGTCCTCAGGTT
AATGGAAGGCTAGAAGCGCAGGGAATTGAGCCAGACTATATTGATGGTATTAGAATCACG
GATGAGCACACAATGGCCGTAGTTAGAAAATGTTTTTTGGAACAAAATCTTAAGCTAGTT
ACTGCATTAGAACAGCTAGGGGTCCGTGCAAGACCCATTACTTCTGGTGTTTTTACTGCT
GACTATTTGGATAAGGACAAATACAAGCTAGTGGGCAATATTAAAAGTGTCACAAAAGAG
CCAATTGAAGCATCTATTAAGGCAGGTGCCCTACCAATCTTGACCTCTTTAGCCGAAACT
GCTTCTGGTCAAATGTTGAACGTCAACGCCGACGTAGCTGCTGGTGAATTAGCCCGTGTT
TTTGAGCCTTTGAAGATCGTTTACCTGAATGAGAAAGGGGGTATTATCAATGGCTCCACG
GGAGAAAAAATTTCGATGATCAATTTGGATGAAGAGTATGACGATTTAATGAAGCAAAGT
TGGGTGAAGTATGGTACCAAATTAAAAATTAGAGAAATTAAAGAGCTTTTGGACTATCTT
CCTCGTTCTTCTTCAGTTGCAATCATTAACGTTCAAGATCTACAAAAAGAACTGTTCACT
GATTCTGGTGCGGGTACTATGATCAGGAGAGGTTACAAATTAGTGAAGAGATCCTCCATT
GGCGAATTTCCATCCGCTGATGCTCTAAGAAAAGCTCTTCAAAGGGACGCTGGCATTAGT
TCCGGTAAAGAATCTGTTGCTTCTTATTTAAGATATTTGGAAAACTCTGATTTTGTCTCT
TATGCTGATGAACCTCTTGAAGCAGTGGCCATTGTAAAGAAAGATACGAACGTTCCCACA
CTAGACAAATTTGTCTGTTCTGACGCAGCCTGGTTGAATAACGTCACAGATAATGTATTC
AATGTTTTGCGCCGTGATTTTCCTGCTTTACAATGGGTAGTCAGTGAAAATGATGCTAAC
ATTGCATGGCATTTTGATAAGTCTCAAGGTTCATATCTAAAAGGCGGAAAAGTTTTGTTC
TGGTATGGTATCGATGATATAAATACAATATCCGAGCTCGTTGAAAATTTTGTGAAGTCG
TGTGACACTGCTTCTACCCTCAACTCATCAGCAAGTAGTGGAGTATTTGCTAACAAAAAA
TCAGCTAGGTCGTACTCAACTAGATCCACTCCTCGTCCCGAGGGAGTTAACACCAACCCT
GGTCGTGTCGCGCTTATTGGTGCTAGAGGTTACACAGGTAAAAATTTGGTATCTTTGATC
AACGGCCACCCATATTTAGAAGTGGCCCATGTTTCTTCTCGTGAATTGAAAGGTCAAAAG
TTGCAAGATTATACAAAATCCGAAATTATATATGAAAGTTTGCAAATACAGGATATTAGG
AAACTGGAAGAACAAAATGCTGTGGACTTTTGGGTTATGGCATTACCCAACAAAGTCTGT
GAACCTTTCGTTGAGACAATCCAAAGTGTTCATGGTAAGTCTAAAATTATTGATCTGTCC
GCTGATCACAGGTTTGTATCAGAATCAGACTGGGCTTACGGTTTGCCAGAATTGAATGAT
AGAGCAAAAATTGCAAACGCTGCCAAAATTGCTAATCCCGGTTGTTATGCTACTGGTTCG
CAATTAACTATTTCTCCGTTAACAAAGTATATCAATGGTCTTCCAACTGTGTTTGGTGTT
TCAGGGTATTCAGGCGCGGGGACGAAGCCTTCTCCAAAAAACGATCCCAAATTCTTGAAC
AATAACTTAATTCCTTACGCTTTAAGTGATCATATACACGAACGCGAAATCTCAGCTCGC
ATTGGGCACAATGTTGCATTCATGCCCCATGTTGGGCAGTGGTTTCAAGGTATCTCTTTG
ACCGTCTCTATTCCAATAAAAAAAGGTTCCTTGTCTATTGATGAGATCAGGAAATTATAC
AGAAATTTTTACGAAGACGAAAAGCTAGTACATGTCATCGATGATATCCCACTGGTTAAA
GATATTGAGGGCACCCATGGTGTAGTTATTGGTGGTTTCAAGCTGAATGATGCTGAAGAT
CGTGTAGTTGTTTGCGCAACCATCGATAACTTACTTAAAGGCGCCGCTACTCAATGTCTG
CAAAATATTAATCTTGCTATGGGTTATGGAGAGTATGCTGGTATCCCTGAAAATAAAATT
ATTGGTGTCTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2592
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism

Gene

Target GenBank: AAB64605

 

Clone

Cloning Information : 112771
HIP Master Clone ID : 88617
Original Clone ID : FLH112771.01X

Organism


Species Specific ID: YER069W