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GEA2 (Saccharomyces cerevisiae) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GEA2
Gene Name:  Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs), involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; similar to but not functionally redundant with Gea1p
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH148823.01X
Keyword: None
Species: Saccharomyces cerevisiae
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
ScCD00024287

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 4380nts         Open reading frame : 1 to 4380

ATGAGTGATAGGGAATTCGTCACGGTAGATCCCGTCACTATCATAATCAAAGAATGCATT
AATTTATCGACAGCGATGCGGAAATACTCTAAATTTACCTCTCAATCTGGAGTGGCCGCT
TTGCTGGGGGGAGGAAGTGAAATATTTAGCAATCAAGATGACTACTTGGCTCACACATTC
AACAATTTGAATACCAACAAGCACAATGATCCATTTTTATCTGGATTCATTCAGTTAAGA
CTTATGTTGAATAAACTGAAAAATCTAGATAATATAGATTCACTAACCATATTGCAGCCA
TTTTTATTAATTGTGAGTACAAGTTCCATTTCTGGTTACATCACTTCCCTGGCCCTGGAC
TCTTTGCAGAAATTCTTTACCTTGAATATCATCAATGAATCATCGCAAAACTATATTGGT
GCACACAGGGCGACGGTAAATGCTCTAACACATTGTAGGTTTGAAGGATCTCAACAACTT
TCTGATGATTCAGTTCTTTTGAAAGTCGTGTTTTTACTGCGTTCAATCGTCGACTCACCT
TACGGAGATTTATTATCAAACTCTATCATATATGACGTATTGCAAACGATTCTTTCATTG
GCTTGTAATAACAGAAGGAGCGAAGTCCTTAGGAATGCTGCACAATCAACAATGATAGCC
GTTACCGTAAAGATTTTCTCAAAACTAAAGACTATTGAGCCTGTTAATGTGAATCAAATA
TACATCAATGATGAAAGTTACACAAATGATGTATTGAAGGCCGATACAATTGGCACAAAT
GTAGAATCCAAAGAAGAAGGAAGTCAAGAAGATCCCATCGGCATGAAAGTGAATAATGAG
GAAGCTATTAGCGAGGACGATGGCATTGAAGAAGAGCATATTCATTCAGAGAAGAGCACA
AATGGCGCCGAACAACTAGATATTGTGCAAAAAACAACAAGATCAAATTCCAGGATCCAA
GCGTATGCTGATGATAACTATGGATTGCCCGTGGTTAGGCAATATTTAAACTTATTACTA
TCATTGATTGCGCCAGAAAATGAATTAAAACATTCATACTCCACTAGAATATTTGGCCTA
GAGTTAATTCAAACGGCATTAGAAATTTCAGGTGATCGATTGCAGCTATACCCACGGCTT
TTTACACTGATATCAGATCCTATTTTCAAAAGCATTTTGTTTATCATACAGAACACTACA
AAATTATCACTACTTCAAGCTACATTGCAGCTATTTACTACTCTAGTTGTTATATTGGGC
AACAACTTACAATTACAGATCGAGCTCACTCTAACAAGAATATTTTCTATTCTTTTAGAT
GATGGTACCGCAAATAACTCGAGTTCTGAAAATAAGAACAAGCCATCAATAATAAAGGAA
CTTCTAATTGAGCAAATATCCATCTTATGGACTAGGTCGCCATCTTTTTTTACTTCTACT
TTTATCAATTTCGATTGTAATCTCGATAGGGCAGACGTTTCCATAAACTTTTTGAAGGCT
TTGACTAAATTGGCCTTACCAGAATCCGCCTTAACTACCACAGAAAGTGTACCACCCATT
TGCCTTGAGGGATTGGTCTCCCTAGTCGATGATATGTTCGATCACATGAAGGACATTGAC
AGAGAAGAATTTGGCAGGCAAAAGAATGAAATGGAAATCTTAAAAAAGAGGGACCGTAAA
ACAGAGTTTATTGAATGTACCAATGCATTCAATGAAAAGCCCAAAAAGGGTATTCCGATG
TTAATAGAAAAAGGTTTCATTGCTTCCGACTCCGATAAAGATATTGCGGAGTTTCTTTTC
AATAATAACAACCGTATGAATAAAAAAACAATCGGTTTGCTACTTTGCCATCCGGACAAA
GTAAGCTTGTTGAATGAATATATTCGTTTGTTTGATTTTTCAGGGTTAAGGGTCGATGAA
GCTATTAGAATTTTGTTGACGAAATTTAGGTTGCCTGGTGAATCGCAACAAATTGAAAGA
ATCATCGAAGCCTTCTCGTCTGCGTATTGTGAAAATCAAGATTACGATCCATCCAAAATC
AGTGACAACGCGGAGGATGACATTTCTACTGTTCAACCAGACGCTGATTCTGTTTTCATT
TTAAGTTATTCAATTATTATGTTGAACACTGACCTACATAACCCTCAAGTGAAGGAACAC
ATGTCATTTGAAGATTACTCTGGTAACTTAAAGGGATGCTGTAATCACAAAGACTTCCCA
TTCTGGTATTTGGATAGAATTTACTGTTCAATCAGAGATAAAGAAATTGTTATGCCTGAA
GAGCACCACGGCAACGAAAAGTGGTTTGAAGATGCTTGGAATAACTTGATATCTTCAACT
ACTGTTATAACTGAAATAAAAAAAGACACACAATCTGTCATGGATAAATTAACACCCTTG
GAGCTTTTGAACTTTGATAGAGCAATTTTTAAACAAGTTGGCCCAAGTATTGTCAGTACT
TTATTCAACATTTACGTAGTTGCATCTGATGACCATATATCTACCAGAATGATAACAAGT
TTGGACAAATGTTCCTATATTTCCGCATTTTTTGACTTCAAAGATCTCTTTAATGATATA
CTAAACTCCATTGCTAAGGGCACTACTTTGATTAATTCAAGCCATGACGATGAACTTTCA
ACTTTAGCTTTTGAATATGGCCCAATGCCACTGGTGCAAATTAAATTCGAAGACACTAAC
ACTGAGATCCCGGTTAGTACAGATGCTGTTAGATTTGGTAGATCATTTAAGGGTCAACTA
AATACAGTTGTTTTTTTCCGGATTATTCGCAGGAACAAAGATCCTAAAATTTTCTCCAAG
GAATTATGGTTAAACATTGTTAATATTATACTAACATTGTACGAAGACTTGATTTTGTCT
CCTGATATTTTCCCTGATTTACAAAAAAGACTGAAATTAAGCAACTTGCCTAAGCCATCT
CCTGAAATTTCTATTAACAAGAGCAAAGAAAGCAAAGGTCTCTTATCAACATTTGCTTCT
TATTTAAAAGGTGATGAAGAACCCACAGAAGAGGAAATCAAATCCTCAAAAAAAGCGATG
GAGTGCATAAAGTCGAGTAATATTGCCGCCTCTGTCTTTGGAAATGAATCAAATATAACA
GCGGATTTAATAAAAACTTTACTAGACTCCGCCAAAACTGAGAAAAACGCAGATAATTCC
AGGTATTTTGAAGCAGAACTTTTATTTATCATCGAATTGACTATTGCATTATTTCTATTT
TGCAAAGAGGAGAAAGAATTAGGAAAGTTCATACTTCAAAAAGTTTTCCAACTTTCTCAC
ACGAAAGGCCTCACGAAAAGGACTGTTCGTAGAATGCTAACATACAAAATTTTGTTAATT
TCGTTATGTGCGGATCAGACGGAGTACTTGTCCAAATTAATAAACGATGAGCTGTTAAAA
AAGGGGGATATTTTTACCCAAAAATTTTTTGCAACTAATCAAGGTAAGGAATTTTTGAAG
AGACTATTTTCATTGACCGAATCAGAGTTTTATAGAGGATTTTTACTAGGAAATGAGAAT
TTTTGGAAATTTTTAAGAAAAGTTACAGCAATGAAAGAGCAGAGCGAGAGCATTTTTGAA
TATTTAAATGAATCGATCAAGACAGACAGCAATATTTTGACAAATGAGAACTTCATGTGG
GTCCTAGGACTATTAGATGAAATTTCATCAATGGGTGCCGTTGGAAATCACTGGGAAATA
GAATACAAGAAATTGACAGAAAGTGGTCATAAAATTGATAAGGAGAATCCATACAAGAAA
TCGATCGAATTATCATTGAAATCCATTCAACTAACATCACACTTGCTGGAAGATAATAAC
GATCTGCGTAAAAACGAGATATTCGCTATTATTCAAGCTTTGGCACATCAATGCATCAAT
CCGTGTAAGCAGATAAGTGAATTTGCAGTGGTAACGCTAGAGCAGACGCTCATCAATAAA
ATCGAAATTCCAACTAATGAGATGGAATCGGTAGAAGAATTAATTGAGGGCGGATTACTA
CCGTTGCTAAATTCGAGTGAAACACAGGAAGACCAGAAAATCCTCATTTCATCCATATTA
ACAATAATTTCAAATGTTTATTTGCATTATTTGAAACTAGGGAAGACAAGCAACGAAACG
TTTTTGAAAATTTTGAGTATTTTCAATAAATTTGTAGAGGACTCAGATATTGAAAAAAAG
CTACAGCAATTAATACTTGATAAGAAGAGTATTGAGAAGGGCAACGGTTCATCATCTCAT
GGATCTGCACATGAACAAACACCAGAGTCAAACGACGTTGAAATTGAGGCTACTGCGCCA
ATTGATGACAATACAGACGATGATAACAAACCGAAGTTATCTGATGTAGAAAAGGATTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 4380
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism

Gene

Target GenBank: AAB64499

 

Protein

SMART domain : Sec7 : Sec7 domain

Clone

Cloning Information : 148823
HIP Master Clone ID : 125142
Original Clone ID : FLH148823.01X

Organism


Species Specific ID: YEL022W