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CCL5 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CCL5
Gene Name:  chemokine (C-C motif) ligand 5
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH023575.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00002558

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 276nts         Open reading frame : 1 to 276
Reference Sequence Alignment

ATGAAGGTCTCCGCGGCAGCCCTCGCTGTCATCCTCATTGCTACTGCCCTCTGCGCTCCT
GCATCTGCCTCCCCATATTCCTCGGACACCACACCCTGCTGCTTTGCCTACATTGCCCGC
CCACTGCCCCGTGCCCACATCAAGGAGTATTTCTACACCAGTGGCAAGTGCTCCAACCCA
GCAGTCGTCTTTGTCACCCGAAAGAACCGCCAAGTGTGTGCCAACCCAGAGAAGAAATGG
GTTCGGGAGTACATCAACTCTTTGGAGATGAGCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 300
Start on reference sequence 25
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CCL5
Symbol Nomenclature : CCL5
SYNONYM : D17S136E; RANTES; SCYA5; SIS-delta; SISd; TCP228; eoCP
Designation : C-C motif chemokine 5|T-cell specific protein p288|beta-chemokine RANTES|eosinophil chemotactic cytokine|regulated upon activation, normally T-expressed, and presumably secreted|small inducible cytokine subfamily A (Cys-Cys), member 5
Full Nomenclature : chemokine (C-C motif) ligand 5
GENEID : 6352
GI : 61371257
GenBank Accession : AY891711
HGNC : 10632
MIM : 187011
Vega : OTTHUMG00000132949
Target GenBank: BC008600

 

Reference Sequence Alignment

  

HsCD00002558        ATGAAGGTCTCCGCGGCAGCCCTCGCTGTCATCCTCATTGCTACTGCCCTCTGCGCTCCT
NM_002985.2         ATGAAGGTCTCCGCGGCAGCCCTCGCTGTCATCCTCATTGCTACTGCCCTCTGCGCTCCT
NM_001278736.1      ATGAAGGTCTCCGCGGCAGCCCTCGCTGTCATCCTCATTGCTACTGCCCTCTGCGCTCCT
                    ************************************************************

HsCD00002558        GCATCTGCCTCCCCATATTCCTCGGACACCACACCCTGCTGCTTTGCCTACATTGCCCGC
NM_002985.2         GCATCTGCCTCCCCATATTCCTCGGACACCACACCCTGCTGCTTTGCCTACATTGCCCGC
NM_001278736.1      GCATCTGCCTCCCCATATTCCTCGGACACCACACCCTGCTGCTTTGCCTACATTGCCCGC
                    ************************************************************

HsCD00002558        CCACTGCCCCGTGCCCACATCAAGGAGTATTTCTACACCAGTGGCAAGTGCTCCAACCCA
NM_002985.2         CCACTGCCCCGTGCCCACATCAAGGAGTATTTCTACACCAGTGGCAAGTGCTCCAACCCA
NM_001278736.1      CCACTGCCCCGTGCCCACATCAAGGAGTATTTCTACACCAGTGGCAAGTGCTCCAACCCA
                    ************************************************************

HsCD00002558        GCAGTCGTCTTTG-----------------------------------------------
NM_002985.2         GCAGTCGTCTTTG-----------------------------------------------
NM_001278736.1      GCAGTCGTCCACAGGTCAAGGATGCCAAAGAGAGAGGGACAGCAAGTCTGGCAGGATTTC
                    ********* : .                                               

HsCD00002558        -----------------------------------TCACCCGAAAGAACCGCCAAGTGTG
NM_002985.2         -----------------------------------TCACCCGAAAGAACCGCCAAGTGTG
NM_001278736.1      CTGTATGACTCCCGGCTGAACAAGGGCAAGCTTTGTCACCCGAAAGAACCGCCAAGTGTG
                                                       *************************

HsCD00002558        TGCCAACCCAGAGAAGAAATGGGTTCGGGAGTACATCAACTCTTTGGAGATGAGCTTG--
NM_002985.2         TGCCAACCCAGAGAAGAAATGGGTTCGGGAGTACATCAACTCTTTGGAGATGAGCTAG--
NM_001278736.1      TGCCAACCCAGAGAAGAAATGGGTTCGGGAGTACATCAACTCTTTGGAGATGAGCTAGGA
                    ********************************************************:*  

HsCD00002558        ------------------------------------------------------------
NM_002985.2         ------------------------------------------------------------
NM_001278736.1      TGGAGAGTCCTTGAACCTGAACTTACACAAATTTGCCTGTTTCTGCTTGCTCTTGTCCTA
                                                                                

HsCD00002558        ---------------------------------------------
NM_002985.2         ---------------------------------------------
NM_001278736.1      GCTTGGGAGGCTTCCCCTCACTATCCTACCCCACCCGCTCCTTGA
                                                                 

Pathways

NCI : Syndecan-1-mediated signaling events
NCI : Syndecan-4-mediated signaling events
NCI : Validated targets of C-MYC transcriptional repression
Panther : Inflammation mediated by chemokine and cytokine signaling pathway
Reactome : Chemokine receptors bind chemokines
Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Peptide ligand-binding receptors
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : EBV LMP1 signaling
WikiPathway : Regulation of toll-like receptor signaling pathway
WikiPathway : TWEAK Signaling Pathway
WikiPathway : Toll-like receptor signaling pathway
WikiPathway : Vitamin B12 Metabolism

Clone

Cloning Information : 23575
HIP Master Clone ID : 53951
Original Clone ID : FLH023575.01L

Organism


TAX_ID : 9606
Species Specific ID: 6352

Genome

Chromosome : 17
Map Location : 17q12
Ensembl : ENSG00000161570

Reagents

Labome : RANTES-antibody