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Detailed Vector Information: pEP Kan-S2

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: pEP Kan-S2
Type: No insert
Vector Name: pEP Kan-S2              
Source: J. Kamil in Coen lab (BCMP/HMS)
Description: Shuttle vector for BAC mutagenesis with CMV promoter, I-SceI sites; kanamycin resistance (neomycin for mammalian selection); restriction enzyme cloning.
Comments: Stored by Jeremy Kamil but not for distribution
Publications: PMID: 16526409
Title: Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli
Authors: B Karsten Tischer
Donald Coen
Jeremy Kamil
Nikolaus Osterrieder
EvNO00025943

Price:  Login for Pricing
NOT AVAILABLE
CPD only
Special MTA: KAM

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
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Vector Name: pEP Kan-S2

pEPKan-S2 in advanced viewed

Synonyms: pEP-Kan-S2, pEP-KanS2
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: Shuttle vector for BAC mutagenesis with CMV promoter, I-SceI sites; kanamycin resistance (neomycin for mammalian selection); restriction enzyme cloning.
Comments: None
Size (bp): 6441
Parent Vector: None
Properties: BAC mutagenesis, I-SceI site, Red recombination, in vitro transcription, mammalian expression, multiple cloning site
Author Name:
Publications: PMID: 16526409
Title: Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS I-SceI site I SceI endonuclease cleavage site 937 954
MCS I-SceI site I SceI endonuclease cleavage site 1925 1942
MCS CpoI site CpoI restriction enzyme site 3789 3795
bacterial origin ColE ColE1-type origin of replication 4668 5350
poly-A signal BGH polyA BGH polyA signal sequence 2034 2261
poly-A signal SV40 polyA SV40 polyA signal sequence 3995 4367
primer site T7 forward T7 primer bind site 863 882
primer site SP6 SP6 primer bind site 1994 2011
primer site 2 micron ori M13 reverse primer bind site 4280 4300
promoter SV40 SV40 promoter/origin of replication 2789 3110
repressor binding site lac operator lacO binding site 4306 4328
selectable marker AmpR ampicillin resistance gene 5448 6107
selectable marker Kan/NeoR neomycin transferase (neoR/kanR) 3149 3940
selectable marker KanR kanamycin resistance gene (kanamycin phosphotransferase) 1078 1890
ssDNA origin f1 f1 origin for ssDNA production 2307 2735
viral origin SV40 ori SV40 promoter/origin of replication 2789 3110

 

Original Clone ID: pEP Kan-S2
Species Specific ID: None
Target GenBank: None