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Detailed Vector Information: pEP-mRFP1-in

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: pEP-mRFP1-in
Type: No insert
Vector Name: pEP-mRFP1-in              
Source: J. Kamil in Coen lab (BCMP/HMS)
Description: Shuttle vector for BAC mutagenesis with CMV tag, disrupted mRFP1 tag, ISceI site; kanamycin resistance (neomycin for mammalian expression); restriction enzyme cloning.
Comments: This clone is NOT a source for an intact mRFP1 open reading frame Stored by Jeremy Kamil but not for distribution
Publications: PMID: 16526409
Title: Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli
Authors: B Karsten Tischer
Donald Coen
Jeremy Kamil
Nikolaus Osterrieder
EvNO00025945

Price:  Login for Pricing
NOT AVAILABLE
CPD only
Special MTA: KAM

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
       DyNA Vector Map

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Vector Name: pEP-mRFP1-in

pEP-mRFP1-in in advanced viewed

Synonyms: pEP-mRFP-in, pEP-mRFP1in, pEPmRFP1-in
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: Suttle vector for BAC mutagenesis with CMV promoter, disrupted mRFP1 tag, I-SceI site; kanamycin resistance (neomycin for mammalian selection); restriction enzyme cloning.
Comments: This vector is NOT a source for the intact mRFP1 open reading frame
Size (bp): 5633
Parent Vector: None
Properties: BAC mutagenesis, I-SceI site, Red recombination, fluorescent marker, mammalian expression, multiple cloning site, ssDNA production, with tag/fusion/marker
Author Name:
Publications: PMID: 16526409
Title: Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS I-SceI site I-SceI endonuclease cleavage site 1014 1031
MCS CpoI site CpoI restriction enzyme site 4172 4178
bacterial origin ColE ColE1-type origin of replication 4855 5537
gene fragment mRFP 1 mRFP1 (fluorescent protein) fragment 1 628 1046
gene fragment mRFP 2 mRFP1 (fluorescent protein) fragment 2 1945 2295
promoter SV40 SV40 early promoter 3298 3494
promoter CMV CMV promoter 1 589
selectable marker KanR kanR (kanamycin phosphotransferase) 1092 1904
selectable marker Kan/NeoR neoR/kanR (neomycin transferase) 3532 4323
ssDNA origin f1 f1 origin for ssDNA production 2559 2999

 

Original Clone ID: pEP-mRFP1-in
Species Specific ID: None
Target GenBank: None