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Detailed Vector Information: pNTAP-TSR

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: pSP72 syn-ProteinC NTAP AphAI
Type: No insert
Vector Name: pNTAP-TSR              
Source: J. Kamil in Coen lab (BCMP/HMS)
Description: Vector with kanR gene insert NTAP tag ORF; useful for two-step Red recombination BAC mutagenesis approach.
Comments: Synthetic affinity tag ORF was designed in order to eliminate repeated elements present in the native CTAP described in PubMed 10504710
Publications: PMID: 16526409
Title: Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli
PMID: 10504710
Title: A generic protein purification method for protein complex characterization and proteome exploration
PMID: 16278461
Title: Highly efficient tandem affinity purification of trypanosome protein complexes based on a novel epitope combination
PMID: 17634236
Title: Human cytomegalovirus protein kinase UL97 forms a complex with the tegument phosphoprotein pp65.
Authors: Donald Coen
Jeremy Kamil
EvNO00025965

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AVAILABLE
CPD only
Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
       DyNA Vector Map

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Vector Name: pNTAP-TSR

pNTAP-TSR in advanced viewed

Synonyms: pSP72-PTP-KanR, pSP72-syn-NTAP-AphAI, pSP72-PTP-AphAI
Description: Vector for BAC mutagenesis via Red recombination (kanR excised upon I-SceI and Rec E/T induction) with disrupted affinity tag (synthetic ORF eliminates nucleotide repeats present in wild-type); kanamycin resistance; restriction enzyme cloning.
Comments: PTP N-terminal TAP tag (see PMID 16278461) added via synthetic construct that does not have direct DNA repeats present in original construct and is codon optimized for human
Size (bp): 3968
Parent Vector: None
Properties: BAC mutagenesis, I-SceI site, Red recombination, bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Publications: PMID: 16526409
Title: Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli

PMID: 10504710
Title: A generic protein purification method for protein complex characterization and proteome exploration

PMID: 17634236
Title: Human cytomegalovirus protein kinase UL97 forms a complex with the tegument phosphoprotein pp65.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position

 

GI: 155197413
GenBank Accession: EU040032
Original Clone ID: pSP72 syn-ProteinC NTAP AphAI
SYNONYM: pSP72 syn-Protein C NTAP AphA
SYNONYM: pSP72-syn-Protein_C-NTAP-AphAI
Species Specific ID: None
Target GenBank: None