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SSNA1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  SSNA1
Gene Name:  Sjogren syndrome nuclear autoantigen 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH012272.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00002600

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 360nts         Open reading frame : 1 to 360
Reference Sequence Alignment

ATGACCCAGCAGGGCGCGGCGCTGCAGAACTACAACAACGAGCTGGTCAAGTGCATAGAG
GAGCTGTGCCAGAAGCGGGAGGAGCTGTGCCGGCAGATCCAGGAGGAGGAGGACGAGAAG
CAGCGGCTGCAGAATGAGGTGAGGCAGCTGACAGAGAAGCTGGCCCGCGTCAACGAGAAC
CTGGCACGCAAGATTGCCTCTCGCAACGAGTTCGACCGGACCATCGCGGAGACGGAGGCC
GCCTACCTCAAGATCCTGGAGAGCTCCCAGACTTTGCTCAGCGTTCTCAAGAGGGAAGCT
GGGAACCTGACCAAGGCTACAGCCCCAGACCAGAAAAGTAGCGGCGGCAGGGACAGCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 440
Start on reference sequence 81
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SSNA1
Symbol Nomenclature : SSNA1
SYNONYM : N14; NA-14; NA14
Designation : Sjoegren syndrome nuclear autoantigen 1|Sjogren's syndrome nuclear autoantigen 1|nuclear autoantigen of 14 kDa
Full Nomenclature : Sjogren syndrome nuclear autoantigen 1
GENEID : 8636
GI : 61371371
GenBank Accession : AY891729
HGNC : 11321
MIM : 610882
Vega : OTTHUMG00000020982
Target GenBank: BC000864

 

Reference Sequence Alignment

  

HsCD00002600      ATGACCCAGCAGGGCGCGGCGCTGCAGAACTACAACAACGAGCTGGTCAAGTGCATAGAG
NM_003731.2       ATGACCCAGCAGGGCGCGGCGCTGCAGAACTACAACAACGAGCTGGTCAAGTGCATAGAG
                  ************************************************************

HsCD00002600      GAGCTGTGCCAGAAGCGGGAGGAGCTGTGCCGGCAGATCCAGGAGGAGGAGGACGAGAAG
NM_003731.2       GAGCTGTGCCAGAAGCGGGAGGAGCTGTGCCGGCAGATCCAGGAGGAGGAGGACGAGAAG
                  ************************************************************

HsCD00002600      CAGCGGCTGCAGAATGAGGTGAGGCAGCTGACAGAGAAGCTGGCCCGCGTCAACGAGAAC
NM_003731.2       CAGCGGCTGCAGAATGAGGTGAGGCAGCTGACAGAGAAGCTGGCCCGCGTCAACGAGAAC
                  ************************************************************

HsCD00002600      CTGGCACGCAAGATTGCCTCTCGCAACGAGTTCGACCGGACCATCGCGGAGACGGAGGCC
NM_003731.2       CTGGCACGCAAGATTGCCTCTCGCAACGAGTTCGACCGGACCATCGCGGAGACGGAGGCC
                  ************************************************************

HsCD00002600      GCCTACCTCAAGATCCTGGAGAGCTCCCAGACTTTGCTCAGCGTTCTCAAGAGGGAAGCT
NM_003731.2       GCCTACCTCAAGATCCTGGAGAGCTCCCAGACTTTGCTCAGCGTTCTCAAGAGGGAAGCT
                  ************************************************************

HsCD00002600      GGGAACCTGACCAAGGCTACAGCCCCAGACCAGAAAAGTAGCGGCGGCAGGGACAGCTTG
NM_003731.2       GGGAACCTGACCAAGGCTACAGCCCCAGACCAGAAAAGTAGCGGCGGCAGGGACAGCTGA
                  ********************************************************** .

Pathways

Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Centrosome maturation
Reactome : G2/M Transition
Reactome : Loss of Nlp from mitotic centrosomes
Reactome : Loss of proteins required for interphase microtubule organizationÿ from the centrosome
Reactome : Mitotic G2-G2/M phases
Reactome : Recruitment of mitotic centrosome proteins and complexes
Reactome : Regulation of PLK1 Activity at G2/M Transition
WikiPathway : Lymphocyte TarBase
WikiPathway : Muscle cell TarBase

Protein

UniProt : O43805
HPRD : 10251

Clone

Cloning Information : 12272
HIP Master Clone ID : 26471
Original Clone ID : FLH012272.01L

Organism


TAX_ID : 9606
Species Specific ID: 8636

Genome

Chromosome : 9
Map Location : 9q34.3
Ensembl : ENSG00000176101

Reagents

Labome : NA14-antibody