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STHM (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ST6GALNAC2
Gene Name:  ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH058449.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00000261

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1125nts         Open reading frame : 1 to 1125
Reference Sequence Alignment

ATGGGGCTCCCGCGCGGGTCGTTCTTCTGGCTGCTGCTCCTGCTCACGGCTGCCTGCTCG
GGGCTCCTCTTTGCCCTGTACTTCTCGGCGGTGCAGCGGTACCCGGGGCCAGCGGCCGGA
GCCAGGGACACCACATCATTTGAAGCATTCTTTCAATCCAAGGCATCGAATTCTTGGACA
GGAAAGGGCCAGGCCTGCCGACACCTGCTTCACCTGGCCATTCAGCGGCACCCCCGCTTC
CGTGGCCTGTTCAATCTCTCCATTCCAGTGCTGCTGTGGGGGGACCTCTTCACCCCAGCG
CTCTGGGACCGCCTGAGCCAACACAAAGCCCCGTATGGCTGGCGGGGGCTCTCTCACCAA
GTCATCGCCTCCACCCTGAGCCTTCTGAACGGCTCAGAGAGTGCCAAGCTGTTTGCCCCG
CCCAGGGACACCCCTCCAAAGTGTATCCGGTGTGCCGTGGTGGGCAACGGAGGCATTCTG
AATGGGTCCCGCCAGGGTCCCAACATCGATGCCCATGACTATGTATTCAGACTCAATGGA
GCTGTGATCAAAGGCTTCGAGCGCGATGTGGGCACCAAGACTTCCTTCTATGGTTTCACT
GTGAATACGATGAAGAACTCCCTCGTCTCCTACTGGAATCTGGGCTTCACCTCCGTGCCA
CAAGGACAGGACCTGCAGTATATCTTCATCCCCTCAGACATCCGCGACTATGTGATGCTG
AGATCGGCCATTCTGGGCGTGCCTGTCCCTGAGGGCCTAGATAAAGGGGACAGGCCGCAC
GCCTATTTTGGACCAGAAGCCTCTGCCAGTAAATTCAAGCTGCTACATCCGGACTTCATC
AGCTACCTGACAGAAAGGTTCTTGAAATCAAAGTTGATTAACACACATTTTGGAGACCTA
TATATGCCTAGTACCGGGGCTCTCATGCTGCTGACAGCTTTGCATACCTGTGACCAGGTC
AGTGCCTATGGATTCATCACAAGCAACTACTGGAAATTTTCCGACCACTATTTCGAACGA
AAAATGAAGCCATTGATATTTTATGCAAACCACGATCTGTCCCTGGAAGCTGCCCTGTGG
AGGGACCTGCACAAGGCCGGCATCCTTCAGCTGTACCAGCGCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1164
Start on reference sequence 40
Mutations:
None
Discrepancy:
a236g,H79R; c606t
                                

5' Linker Sequence:
GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
(goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ST6GALNAC2
Symbol Nomenclature : ST6GALNAC2
SYNONYM : SAITL1; SIAT7; SIAT7B; SIATL1; ST6GalNAII; STHM
Designation : (alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide B|(alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide alpha-2,6-sialyltransferase|SIAT7-B|ST6GalNAc II|ST6GalNAcII|alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2|galNAc alpha-2,6-sialyltransferase II|sialyltransferase 7 ((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide B|sialyltransferase 7 ((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide alpha-2,6-sialyltransferase) B|sialyltransferase 7B|sialyltransferase-like 1
Full Nomenclature : ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2
GENEID : 10610
GI : 60814053
GenBank Accession : AY893248
HGNC : 10867
MIM : 610137
Vega : OTTHUMG00000180301
Target GenBank: AJ251053

 

Reference Sequence Alignment

  

HsCD00000261      ATGGGGCTCCCGCGCGGGTCGTTCTTCTGGCTGCTGCTCCTGCTCACGGCTGCCTGCTCG
NM_006456.2       ATGGGGCTCCCGCGCGGGTCGTTCTTCTGGCTGCTGCTCCTGCTCACGGCTGCCTGCTCG
                  ************************************************************

HsCD00000261      GGGCTCCTCTTTGCCCTGTACTTCTCGGCGGTGCAGCGGTACCCGGGGCCAGCGGCCGGA
NM_006456.2       GGGCTCCTCTTTGCCCTGTACTTCTCGGCGGTGCAGCGGTACCCGGGGCCAGCGGCCGGA
                  ************************************************************

HsCD00000261      GCCAGGGACACCACATCATTTGAAGCATTCTTTCAATCCAAGGCATCGAATTCTTGGACA
NM_006456.2       GCCAGGGACACCACATCATTTGAAGCATTCTTTCAATCCAAGGCATCGAATTCTTGGACA
                  ************************************************************

HsCD00000261      GGAAAGGGCCAGGCCTGCCGACACCTGCTTCACCTGGCCATTCAGCGGCACCCCCGCTTC
NM_006456.2       GGAAAGGGCCAGGCCTGCCGACACCTGCTTCACCTGGCCATTCAGCGGCACCCCCACTTC
                  *******************************************************.****

HsCD00000261      CGTGGCCTGTTCAATCTCTCCATTCCAGTGCTGCTGTGGGGGGACCTCTTCACCCCAGCG
NM_006456.2       CGTGGCCTGTTCAATCTCTCCATTCCAGTGCTGCTGTGGGGGGACCTCTTCACCCCAGCG
                  ************************************************************

HsCD00000261      CTCTGGGACCGCCTGAGCCAACACAAAGCCCCGTATGGCTGGCGGGGGCTCTCTCACCAA
NM_006456.2       CTCTGGGACCGCCTGAGCCAACACAAAGCCCCGTATGGCTGGCGGGGGCTCTCTCACCAA
                  ************************************************************

HsCD00000261      GTCATCGCCTCCACCCTGAGCCTTCTGAACGGCTCAGAGAGTGCCAAGCTGTTTGCCCCG
NM_006456.2       GTCATCGCCTCCACCCTGAGCCTTCTGAACGGCTCAGAGAGTGCCAAGCTGTTTGCCCCG
                  ************************************************************

HsCD00000261      CCCAGGGACACCCCTCCAAAGTGTATCCGGTGTGCCGTGGTGGGCAACGGAGGCATTCTG
NM_006456.2       CCCAGGGACACCCCTCCAAAGTGTATCCGGTGTGCCGTGGTGGGCAACGGAGGCATTCTG
                  ************************************************************

HsCD00000261      AATGGGTCCCGCCAGGGTCCCAACATCGATGCCCATGACTATGTATTCAGACTCAATGGA
NM_006456.2       AATGGGTCCCGCCAGGGTCCCAACATCGATGCCCATGACTATGTATTCAGACTCAATGGA
                  ************************************************************

HsCD00000261      GCTGTGATCAAAGGCTTCGAGCGCGATGTGGGCACCAAGACTTCCTTCTATGGTTTCACT
NM_006456.2       GCTGTGATCAAAGGCTTCGAGCGCGATGTGGGCACCAAGACTTCCTTCTATGGTTTCACT
                  ************************************************************

HsCD00000261      GTGAATACGATGAAGAACTCCCTCGTCTCCTACTGGAATCTGGGCTTCACCTCCGTGCCA
NM_006456.2       GTGAACACGATGAAGAACTCCCTCGTCTCCTACTGGAATCTGGGCTTCACCTCCGTGCCA
                  ***** ******************************************************

HsCD00000261      CAAGGACAGGACCTGCAGTATATCTTCATCCCCTCAGACATCCGCGACTATGTGATGCTG
NM_006456.2       CAAGGACAGGACCTGCAGTATATCTTCATCCCCTCAGACATCCGCGACTATGTGATGCTG
                  ************************************************************

HsCD00000261      AGATCGGCCATTCTGGGCGTGCCTGTCCCTGAGGGCCTAGATAAAGGGGACAGGCCGCAC
NM_006456.2       AGATCGGCCATTCTGGGCGTGCCTGTCCCTGAGGGCCTAGATAAAGGGGACAGGCCGCAC
                  ************************************************************

HsCD00000261      GCCTATTTTGGACCAGAAGCCTCTGCCAGTAAATTCAAGCTGCTACATCCGGACTTCATC
NM_006456.2       GCCTATTTTGGACCAGAAGCCTCTGCCAGTAAATTCAAGCTGCTACATCCGGACTTCATC
                  ************************************************************

HsCD00000261      AGCTACCTGACAGAAAGGTTCTTGAAATCAAAGTTGATTAACACACATTTTGGAGACCTA
NM_006456.2       AGCTACCTGACAGAAAGGTTCTTGAAATCAAAGTTGATTAACACACATTTTGGAGACCTA
                  ************************************************************

HsCD00000261      TATATGCCTAGTACCGGGGCTCTCATGCTGCTGACAGCTTTGCATACCTGTGACCAGGTC
NM_006456.2       TATATGCCTAGTACCGGGGCTCTCATGCTGCTGACAGCTTTGCATACCTGTGACCAGGTC
                  ************************************************************

HsCD00000261      AGTGCCTATGGATTCATCACAAGCAACTACTGGAAATTTTCCGACCACTATTTCGAACGA
NM_006456.2       AGTGCCTATGGATTCATCACAAGCAACTACTGGAAATTTTCCGACCACTATTTCGAACGA
                  ************************************************************

HsCD00000261      AAAATGAAGCCATTGATATTTTATGCAAACCACGATCTGTCCCTGGAAGCTGCCCTGTGG
NM_006456.2       AAAATGAAGCCATTGATATTTTATGCAAACCACGATCTGTCCCTGGAAGCTGCCCTGTGG
                  ************************************************************

HsCD00000261      AGGGACCTGCACAAGGCCGGCATCCTTCAGCTGTACCAGCGCTAG
NM_006456.2       AGGGACCTGCACAAGGCCGGCATCCTTCAGCTGTACCAGCGCTGA
                  *******************************************..

Pathways

Reactome : Asparagine N-linked glycosylation
Reactome : Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome : Metabolism of proteins
Reactome : O-linked glycosylation
Reactome : O-linked glycosylation of mucins
Reactome : Post-translational protein modification
Reactome : Sialic acid metabolism
Reactome : Synthesis of substrates in N-glycan biosythesis
Reactome : Termination of O-glycan biosynthesis

Protein

UniProt : Q9UJ37
HPRD : 10231

Clone

Cloning Information : 58449
HIP Master Clone ID : 39009
Original Clone ID : FLH058449.01X

Organism


TAX_ID : 9606
Species Specific ID: 10610

Genome

Chromosome : 17
Map Location : 17q25.1
Ensembl : ENSG00000070731

Reagents

Labome : ST6GALNAC2-antibody