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SFRS6 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  SRSF6
Gene Name:  serine/arginine-rich splicing factor 6
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH026479.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00002619

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1035nts         Open reading frame : 1 to 1035
Reference Sequence Alignment

ATGCCGCGCGTCTACATAGGACGCCTGAGCTACAACGTCCGGGAGAAGGACATCCAGCGC
TTTTTCAGTGGCTATGGCCGCCTCCTCGAAGTAGACCTCAAAAATGGGTACGGCTTCGTG
GAGTTCGAGGACTCCCGCGACGCCGACGACGCCGTTTACGAGCTGAACGGCAAGGAGCTC
TGCGGCGAGCGCGTGATCGTAGAGCACGCCCGGGGCCCGCGTCGCGATCGCGACGGCTAC
AGCTACGGAAGCCGCAGTGGTGGAGGTGGATACAGCAGTCGGAGAACATCTGGCAGAGAC
AAATACGGACCACCTGTTCGTACAGAATACAGGCTTATTGTAGAAAATCTTTCTAGTCGG
TGCAGTTGGCAAGATTTAAAGGATTTTATGCGACAAGCAGGTGAAGTAACCTATGCGGAT
GCCCACAAGGAACGAACAAATGAGGGTGTAATTGAGTTTCGCTCCTACTCTGACATGAAG
CGTGCTTTGGACAAACTGGATGGCACAGAAATAAATGGCAGAAATATTAGGCTTATTGAA
GATAAGCCACGCACAAGCCATAGGCGATCTTACTCTGGAAGCAGATCCAGGTCTCGATCT
AGAAGACGGTCACGAAGTAGGAGTCGCAGGAGCAGCCGCAGTAGATCTCGAAGTATCTCA
AAAAGTCGCTCCCGTTCCAGGTCGCGGAGCAAAGGTCGATCACGTTCTCGATCAAAAGGC
AGGAAATCTAGATCAAAGAGCAAATCTAAGCCCAAGTCTGATCGGGGCTCCCATTCACAT
TCTCGAAGCAGATCTAAGGATGAGTATGAGAAATCTCGAAGCAGGTCTCGGTCCCGATCC
CCCAAAGAAAATGGAAAGGGTGATATAAAGTCAAAATCCAGATCAAGGAGCCAGTCCCGT
TCCAATTCGCCGCTACCTGTTCCACCCTCAAAGGCCCGTTCTGTGTCCCCTCCACCAAAA
AGAGCTACTTCAAGATCCCGTTCTAGATCTCGCTCAAAGTCAAGATCAAGGTCCAGGTCG
AGTTCCAGAGATTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1171
Start on reference sequence 137
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SRSF6
Symbol Nomenclature : SRSF6
SYNONYM : B52; SFRS6; SRP55
Designation : SR splicing factor 6|pre-mRNA-splicing factor SRP55|splicing factor, arginine/serine-rich, 55 kDa
Full Nomenclature : serine/arginine-rich splicing factor 6
GENEID : 6431
GI : 61371421
GenBank Accession : AY891737
HGNC : 10788
MIM : 601944
Vega : OTTHUMG00000032502
Target GenBank: BC006832

 

Reference Sequence Alignment

  

HsCD00002619      ATGCCGCGCGTCTACATAGGACGCCTGAGCTACAACGTCCGGGAGAAGGACATCCAGCGC
NM_006275.5       ATGCCGCGCGTCTACATAGGACGCCTGAGCTACAACGTCCGGGAGAAGGACATCCAGCGC
                  ************************************************************

HsCD00002619      TTTTTCAGTGGCTATGGCCGCCTCCTCGAAGTAGACCTCAAAAATGGGTACGGCTTCGTG
NM_006275.5       TTTTTCAGTGGCTATGGCCGCCTCCTCGAAGTAGACCTCAAAAATGGGTACGGCTTCGTG
                  ************************************************************

HsCD00002619      GAGTTCGAGGACTCCCGCGACGCCGACGACGCCGTTTACGAGCTGAACGGCAAGGAGCTC
NM_006275.5       GAGTTCGAGGACTCCCGCGACGCCGACGACGCCGTTTACGAGCTGAACGGCAAGGAGCTC
                  ************************************************************

HsCD00002619      TGCGGCGAGCGCGTGATCGTAGAGCACGCCCGGGGCCCGCGTCGCGATCGCGACGGCTAC
NM_006275.5       TGCGGCGAGCGCGTGATCGTAGAGCACGCCCGGGGCCCGCGTCGCGATCGCGACGGCTAC
                  ************************************************************

HsCD00002619      AGCTACGGAAGCCGCAGTGGTGGAGGTGGATACAGCAGTCGGAGAACATCTGGCAGAGAC
NM_006275.5       AGCTACGGAAGCCGCAGTGGTGGAGGTGGATACAGCAGTCGGAGAACATCTGGCAGAGAC
                  ************************************************************

HsCD00002619      AAATACGGACCACCTGTTCGTACAGAATACAGGCTTATTGTAGAAAATCTTTCTAGTCGG
NM_006275.5       AAATACGGACCACCTGTTCGTACAGAATACAGGCTTATTGTAGAAAATCTTTCTAGTCGG
                  ************************************************************

HsCD00002619      TGCAGTTGGCAAGATTTAAAGGATTTTATGCGACAAGCAGGTGAAGTAACCTATGCGGAT
NM_006275.5       TGCAGTTGGCAAGATTTAAAGGATTTTATGCGACAAGCAGGTGAAGTAACCTATGCGGAT
                  ************************************************************

HsCD00002619      GCCCACAAGGAACGAACAAATGAGGGTGTAATTGAGTTTCGCTCCTACTCTGACATGAAG
NM_006275.5       GCCCACAAGGAACGAACAAATGAGGGTGTAATTGAGTTTCGCTCCTACTCTGACATGAAG
                  ************************************************************

HsCD00002619      CGTGCTTTGGACAAACTGGATGGCACAGAAATAAATGGCAGAAATATTAGGCTTATTGAA
NM_006275.5       CGTGCTTTGGACAAACTGGATGGCACAGAAATAAATGGCAGAAATATTAGGCTTATTGAA
                  ************************************************************

HsCD00002619      GATAAGCCACGCACAAGCCATAGGCGATCTTACTCTGGAAGCAGATCCAGGTCTCGATCT
NM_006275.5       GATAAGCCACGCACAAGCCATAGGCGATCTTACTCTGGAAGCAGATCCAGGTCTCGATCT
                  ************************************************************

HsCD00002619      AGAAGACGGTCACGAAGTAGGAGTCGCAGGAGCAGCCGCAGTAGATCTCGAAGTATCTCA
NM_006275.5       AGAAGACGGTCACGAAGTAGGAGTCGCAGGAGCAGCCGCAGTAGATCTCGAAGTATCTCA
                  ************************************************************

HsCD00002619      AAAAGTCGCTCCCGTTCCAGGTCGCGGAGCAAAGGTCGATCACGTTCTCGATCAAAAGGC
NM_006275.5       AAAAGTCGCTCCCGTTCCAGGTCGCGGAGCAAAGGTCGATCACGTTCTCGATCAAAAGGC
                  ************************************************************

HsCD00002619      AGGAAATCTAGATCAAAGAGCAAATCTAAGCCCAAGTCTGATCGGGGCTCCCATTCACAT
NM_006275.5       AGGAAATCTAGATCAAAGAGCAAATCTAAGCCCAAGTCTGATCGGGGCTCCCATTCACAT
                  ************************************************************

HsCD00002619      TCTCGAAGCAGATCTAAGGATGAGTATGAGAAATCTCGAAGCAGGTCTCGGTCCCGATCC
NM_006275.5       TCTCGAAGCAGATCTAAGGATGAGTATGAGAAATCTCGAAGCAGGTCTCGGTCCCGATCC
                  ************************************************************

HsCD00002619      CCCAAAGAAAATGGAAAGGGTGATATAAAGTCAAAATCCAGATCAAGGAGCCAGTCCCGT
NM_006275.5       CCTAAAGAAAATGGAAAGGGTGATATAAAGTCAAAATCCAGATCAAGGAGCCAGTCCCGT
                  ** *********************************************************

HsCD00002619      TCCAATTCGCCGCTACCTGTTCCACCCTCAAAGGCCCGTTCTGTGTCCCCTCCACCAAAA
NM_006275.5       TCCAATTCGCCGCTACCTGTTCCACCCTCAAAGGCCCGTTCTGTGTCCCCTCCACCAAAA
                  ************************************************************

HsCD00002619      AGAGCTACTTCAAGATCCCGTTCTAGATCTCGCTCAAAGTCAAGATCAAGGTCCAGGTCG
NM_006275.5       AGAGCTACTTCAAGATCCCGTTCTAGATCTCGCTCAAAGTCAAGATCAAGGTCCAGGTCG
                  ************************************************************

HsCD00002619      AGTTCCAGAGATTTG
NM_006275.5       AGTTCCAGAGATTAA
                  *************:.

Pathways

Reactome : Cleavage of Growing Transcript in the Termination Region
Reactome : Gene Expression
Reactome : Post-Elongation Processing of Intron-Containing pre-mRNA
Reactome : Post-Elongation Processing of the Transcript
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Termination
Reactome : Transcription
Reactome : Transport of Mature Transcript to Cytoplasm
Reactome : Transport of Mature mRNA derived from an Intron-Containing Transcript
Reactome : mRNA 3'-end processing
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway
Reactome : mRNA Splicing - Minor Pathway
WikiPathway : mRNA processing

Protein

SMART domain : RRM : RNA recognition motif
UniProt : Q13247
HPRD : 09054

Clone

Cloning Information : 26479
HIP Master Clone ID : 56855
Original Clone ID : FLH026479.01L

Organism


TAX_ID : 9606
Species Specific ID: 6431

Genome

Chromosome : 20
Map Location : 20q12-q13.1
Ensembl : ENSG00000124193

Reagents

Labome : SRSF6-antibody