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MNAT1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  MNAT1
Gene Name:  MNAT CDK-activating kinase assembly factor 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH024230.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00002637

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 930nts         Open reading frame : 1 to 930
Reference Sequence Alignment

ATGGACGATCAGGGTTGCCCTCGGTGTAAGACCACCAAATATCGGAACCCCTCCTTGAAG
CTGATGGTGAATGTGTGCGGACACACTCTCTGTGAAAGTTGTGTAGATTTACTGTTTGTG
AGAGGAGCTGGAAACTGCCCTGAGTGTGGTACTCCACTCAGAAAGAGCAACTTCAGGGTA
CAACTCTTTGAAGATCCCACTGTTGACAAGGAGGTTGAGATCAGGAAAAAAGTGCTAAAG
ATATACAATAAAAGGGAAGAAGATTTTCCTAGTCTAAGAGAATACAATGATTTCTTGGAA
GAAGTGGAAGAAATTGTTTTCAACTTGACCAACAATGTGGATTTGGACAACACCAAAAAG
AAAATGGAGATATACCAAAAGGAAAACAAAGATGTTATTCAGAAAAATAAATTAAAGCTG
ACTCGAGAACAGGAAGAACTGGAAGAAGCTTTAGAAGTGGAACGACAGGAAAATGAACAA
AGAAGATTATTTATACAAAAAGAAGAACAACTGCAGCAGATTCTAAAAAGGAAGAATAAG
CAGGCTTTTTTAGATGAGCTGGAGAGTTCTGATCTCCCTGTTGCTCTGCTTTTGGCTCAG
CATAAAGATAGATCTACCCAATTAGAAATGCAACTTGAGAAACCCAAACCTGTAAAACCA
GTGACGTTTTCCACAGGCATCAAAATGGGTCAACATATTTCACTGGCACCTATTCACAAG
CTTGAAGAAGCTCTGTATGAATACCAGCCACTGCAGATAGAGACATATGGACCACATGTT
CCTGAGCTTGAGATGCTAGGAAGACTTGGGTATTTAAACCATGTCAGAGCTGCCTCACCA
CAGGACCTTGCTGGAGGCTATACTTCTTCTCTTGCTTGTCACAGAGCACTACAGGATGCA
TTCAGTGGGCTTTTCTGGCAGCCCAGTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1031
Start on reference sequence 102
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MNAT1
Symbol Nomenclature : MNAT1
SYNONYM : CAP35; MAT1; RNF66; TFB3
Designation : CDK-activating kinase assembly factor MAT1|CDK7/cyclin-H assembly factor|RING finger protein 66|RING finger protein MAT1|cyclin G1 interacting protein|cyclin-G1-interacting protein|menage a trois 1 (CAK assembly factor)|menage a trois homolog 1, cyclin H assembly factor
Full Nomenclature : MNAT CDK-activating kinase assembly factor 1
GENEID : 4331
GI : 61371457
GenBank Accession : AY891744
HGNC : 7181
MIM : 602659
Vega : OTTHUMG00000152340
Target GenBank: BC000820

 

Reference Sequence Alignment

  

HsCD00002637        ATGGACGATCAGGGTTGCCCTCGGTGTAAGACCACCAAATATCGGAACCCCTCCTTGAAG
XM_005267687.1      ATGGACGATCAGGGTTGCCCTCGGTGTAAGACCACCAAATATCGGAACCCCTCCTTGAAG
NM_002431.3         ATGGACGATCAGGGTTGCCCTCGGTGTAAGACCACCAAATATCGGAACCCCTCCTTGAAG
                    ************************************************************

HsCD00002637        CTGATGGTGAATGTGTGCGGACACACTCTCTGTGAAAGTTGTGTAGATTTACTGTTTGTG
XM_005267687.1      CTGATGGTGAATGTGTGCGGACACACTCTCTGTGAAAGTTGTGTAGATTTACTGTTTGTG
NM_002431.3         CTGATGGTGAATGTGTGCGGACACACTCTCTGTGAAAGTTGTGTAGATTTACTGTTTGTG
                    ************************************************************

HsCD00002637        AGAGGAGCTGGAAACTGCCCTGAGTGTGGTACTCCACTCAGAAAGAGCAACTTCAGGGTA
XM_005267687.1      AGAGGAGCTGGAAACTGCCCTGAGTGTGGTACTCCACTCAGAAAGAGCAACTTCAGGGTA
NM_002431.3         AGAGGAGCTGGAAACTGCCCTGAGTGTGGTACTCCACTCAGAAAGAGCAACTTCAGGGTA
                    ************************************************************

HsCD00002637        CAACTCTTTGAAGATCCCACTGTTGACAAGGAGGTTGAGATCAGGAAAAAAGTGCTAAAG
XM_005267687.1      CAACTCTTTGAAGATCCCACTGTTGACAAGGAGGTTGAGATCAGGAAAAAAGTGCTAAAG
NM_002431.3         CAACTCTTTGAAGATCCCACTGTTGACAAGGAGGTTGAGATCAGGAAAAAAGTGCTAAAG
                    ************************************************************

HsCD00002637        ATATACAATAAAAGGGAAGAAGATTTTCCTAGTCTAAGAGAATACAATGATTTCTTGGAA
XM_005267687.1      ATATACAATAAAAGGGAAGAAGATTTTCCTAGTCTAAGAGAATACAATGATTTCTTGGAA
NM_002431.3         ATATACAATAAAAGGGAAGAAGATTTTCCTAGTCTAAGAGAATACAATGATTTCTTGGAA
                    ************************************************************

HsCD00002637        GAAGTGGAAGAAATTGTTTTCAACTTGACCAACAATGTGGATTTGGACAACACCAAAAAG
XM_005267687.1      GAAGTGGAAGAAATTGTTTTCAACTTGACCAACAATGTGGATTTGGACAACACCAAAAAG
NM_002431.3         GAAGTGGAAGAAATTGTTTTCAACTTGACCAACAATGTGGATTTGGACAACACCAAAAAG
                    ************************************************************

HsCD00002637        AAAATGGAGATATACCAAAAGGAAAACAAAGATGTTATTCAGAAAAATAAATTAAAGCTG
XM_005267687.1      AAAATGGAGATATACCAAAAGGAAAACAAAGATGTTATTCAGAAAAATAAATTAAAGCTG
NM_002431.3         AAAATGGAGATATACCAAAAGGAAAACAAAGATGTTATTCAGAAAAATAAATTAAAGCTG
                    ************************************************************

HsCD00002637        ACTCGAGAACAGGAAGAACTGGAAGAAGCTTTAGAAGTGGAACGACAGGAAAATGAACAA
XM_005267687.1      ACTCGAGAACAGGAAGAACTGGAAGAAGCTTTAGAAGTGGAACGACAGGAAAATGAACAA
NM_002431.3         ACTCGAGAACAGGAAGAACTGGAAGAAGCTTTAGAAGTGGAACGACAGGAAAATGAACAA
                    ************************************************************

HsCD00002637        AGAAGATTATTTATACAAAAAGAAGAACAACTGCAGCAGATTCTAAAAAGGAAGAATAAG
XM_005267687.1      AGAAGATTATTTATACAAAAAGAAGAACAACTGCAGCAGATTCTAAAAAGGAAGAATAAG
NM_002431.3         AGAAGATTATTTATACAAAAAGAAGAACAACTGCAGCAGATTCTAAAAAGGAAGAATAAG
                    ************************************************************

HsCD00002637        CAGGCTTTTTTAGATGAGCTGGAGAGTTCTGATCTCCCTGTTGCTCTGCTTTTGGCTCAG
XM_005267687.1      CAGGCTTTTTTAGATGAGCTGGAGAGTTCTGATCTCCCTGTTGCTCTGCTTTTGGCTCAG
NM_002431.3         CAGGCTTTTTTAGATGAGCTGGAGAGTTCTGATCTCCCTGTTGCTCTGCTTTTGGCTCAG
                    ************************************************************

HsCD00002637        CATAAAGATAGATCTACCCAATTAGAAATGCAACTTGAGAAACCCAAACCTGTAAAACCA
XM_005267687.1      CATAAAGATAGATCTACCCAATTAGAAATGCAACTTGAGAAACCCAAACCTGTAAAACCA
NM_002431.3         CATAAAGATAGATCTACCCAATTAGAAATGCAACTTGAGAAACCCAAACCTGTAAAACCA
                    ************************************************************

HsCD00002637        GTGACGTTTTCCACAGGCATCAAAATGGGTCAACATATTTCACTGGCACCTATTCACAAG
XM_005267687.1      GTGACGTTTTCCACAGGCATCAAAATGGGTCAACATATTTCACTGGCACCTATTCACAAG
NM_002431.3         GTGACGTTTTCCACAGGCATCAAAATGGGTCAACATATTTCACTGGCACCTATTCACAAG
                    ************************************************************

HsCD00002637        CTTGAAGAAGCTCTGTATGAATACCAGCCACTGCAGATAGAGACATATGGACCACATGTT
XM_005267687.1      CTTGAAGAAGCTCTGTATGAATACCAGCCACTGCAGATAGAGACATATGGACCACATGTT
NM_002431.3         CTTGAAGAAGCTCTGTATGAATACCAGCCACTGCAGATAGAGACATATGGACCACATGTT
                    ************************************************************

HsCD00002637        CCTGAGCTTGAGATGCTAGGAAGACTTGGGTATTTAAACCATGTCAGAGCTGCCTCACCA
XM_005267687.1      CCTGAGCTTGAGATGCTAGGAAGACTTGGGTATTTAAACCATGTCAGAGCTGCCTCACCA
NM_002431.3         CCTGAGCTTGAGATGCTAGGAAGACTTGGGTATTTAAACCATGTCAGAGCTGCCTCACCA
                    ************************************************************

HsCD00002637        CAGGACCTTGCTGGAGGCTATACTTCTTCTCTTGCTTGTCACAGAGCACTACAGGATGCA
XM_005267687.1      CAGGACCTTGCTGGAGGCTATACTTCTTCTCTTGCTTGTCACAGAGCACTACAGGATGCA
NM_002431.3         CAGGACCTTGCTGGAGGCTATACTTCTTCTCTTGCTTGTCACAGAGCACTACAGGATGCA
                    ************************************************************

HsCD00002637        TTCAGTGGGCTTTTCTGGCAGCCCAGTTTG
XM_005267687.1      TTCAGTGGGCTTTTCTGGCAGCCCAGTTAA
NM_002431.3         TTCAGTGGGCTTTTCTGGCAGCCCAGTTAA
                    ****************************:.

Pathways

NCI : Retinoic acid receptors-mediated signaling
Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Cyclin A/B1 associated events during G2/M transition
Reactome : Cyclin A:Cdk2-associated events at S phase entry
Reactome : Cyclin D associated events in G1
Reactome : Cyclin E associated events during G1/S transition
Reactome : DNA Repair
Reactome : Disease
Reactome : Dual incision reaction in GG-NER
Reactome : Dual incision reaction in TC-NER
Reactome : Epigenetic regulation of gene expression
Reactome : Formation of HIV elongation complex in the absence of HIV Tat
Reactome : Formation of HIV-1 elongation complex containing HIV-1 Tat
Reactome : Formation of RNA Pol II elongation complex
Reactome : Formation of incision complex in GG-NER
Reactome : Formation of the Early Elongation Complex
Reactome : Formation of the HIV-1 Early Elongation Complex
Reactome : Formation of transcription-coupled NER (TC-NER) repair complex
Reactome : G1 Phase
Reactome : G1/S Transition
Reactome : G2/M Transition
Reactome : Gene Expression
Reactome : Global Genomic NER (GG-NER)
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : HIV Transcription Elongation
Reactome : HIV Transcription Initiation
Reactome : Late Phase of HIV Life Cycle
Reactome : Mitotic G1-G1/S phases
Reactome : Mitotic G2-G2/M phases
Reactome : Negative epigenetic regulation of rRNA expression
Reactome : NoRC negatively regulates rRNA expression
Reactome : Nucleotide Excision Repair
Reactome : RNA Pol II CTD phosphorylation and interaction with CE
Reactome : RNA Polymerase I Chain Elongation
Reactome : RNA Polymerase I Promoter Clearance
Reactome : RNA Polymerase I Promoter Escape
Reactome : RNA Polymerase I Transcription
Reactome : RNA Polymerase I Transcription Initiation
Reactome : RNA Polymerase I Transcription Termination
Reactome : RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
Reactome : RNA Polymerase II HIV Promoter Escape
Reactome : RNA Polymerase II Pre-transcription Events
Reactome : RNA Polymerase II Promoter Escape
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Elongation
Reactome : RNA Polymerase II Transcription Initiation
Reactome : RNA Polymerase II Transcription Initiation And Promoter Clearance
Reactome : RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
Reactome : S Phase
Reactome : Tat-mediated elongation of the HIV-1 transcript
Reactome : Transcription
Reactome : Transcription of the HIV genome
Reactome : Transcription-coupled NER (TC-NER)
Reactome : mRNA Capping
WikiPathway : Eukaryotic Transcription Initiation
WikiPathway : G1 to S cell cycle control

Protein

SMART domain : RING : Ring finger
UniProt : P51948
HPRD : 04042

Clone

Cloning Information : 24230
HIP Master Clone ID : 54606
Original Clone ID : FLH024230.01L

Organism


TAX_ID : 9606
Species Specific ID: 4331

Genome

Chromosome : 14
Map Location : 14q23
Ensembl : ENSG00000020426

Reagents

Labome : MNAT1-antibody