TM0935 (T. maritima ) in pMH1 (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  TM0935
Gene Name:  hypothetical protein
Original Clone ID:
7528
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH1
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 15326606
Title: Crystal structure of a tandem cystathionine-beta-synthase (CBS) domain protein (TM0935) from Thermotoga maritima at 1.87 A resolution.
Authors:
PSI
Joint Center for Structural Genomics
TmCD00282177
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 438nts
Open reading frame: 51 to 488

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGAAAGTGA
AAGACGTCTGCAAATTGATTTCTCTGAAGCCCACAGTTGTAGAGGAAGACACTCCTATAG
AAGAAATCGTCGACAGGATACTGGAGGATCCCGTCACAAGAACCGTTTATGTTGCGAGAG
ACAACAAACTCGTTGGAATGATCCCTGTCATGCACCTTTTGAAGGTTTCTGGTTTTCACT
TTTTCGGGTTCATTCCAAAGGAAGAGCTCATTCGCTCCTCGATGAAAAGATTGATAGCAA
AGAACGCATCTGAGATAATGTTGGATCCTGTATACGTTCACATGGATACACCATTGGAAG
AAGCCTTGAAGCTGATGATCGACAACAATATACAGGAGATGCCGGTTGTCGATGAAAAGG
GAGAGATAGTGGGAGATCTCAACTCTCTCGAAATTCTTCTCGCTTTGTGGAAAGGGCGGG
AGAAATAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTT

Protein sequence predicted based on nucleotide sequence

MKVKDVCKLISLKPTVVEEDTPIEEIVDRILEDPVTRTVYVARDNKLVGMIPVMHLLKVS
GFHFFGFIPKEELIRSSMKRLIAKNASEIMLDPVYVHMDTPLEEALKLMIDNNIQEMPVV
DEKGEIVGDLNSLEILLALWKGREK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 438
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 282804
Recommended expression in:
Not Applicable