DNASU Plasmid Repository • 480.965.5697 | Email

CFTR (Homo sapiens) in pFASTBAC1-C-His-TOPO (His-tagged baculovirus expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  CFTR
Gene Name:  cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Sequence             
Original Clone ID: 2935c552KWg2h2
Keyword: Partial Protein, NBD1 domain
Species: Homo sapiens
Type: cDNA
Vector Name: pFASTBAC1-C-His-TOPO               Format:  FUSION
Source: SGX Pharmaceuticals, Inc.
Description: This clone encodes the NBD1 domain with other intended mutations and corresponds to region 388-646 on full length protein NP_000483.3.
Comments: C-terminal 6-His, TOPO. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation/
Discrepancy :
Yes / Unknown
Publications: None
Authors: SGX Pharmaceuticals, Inc.
HsCD00287209

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 816nts         Open reading frame : 1 to 816

ATGGCCCTTACAAGCTTAAAAGGACTATGGACACTTCGTGCCTTCGGACGGCAGCCTTCC
GGTGGTGGTAGCGGAGGAGGATCTGGCGGCGGCTCAGGGGGCGGGACGACTACAGAAGTA
GTGATGGAGAATGTAACAGCCTTCTGGGAGGAGGGAGGTACTCCTGTCCTGAAAGATATT
AATTTCAAGATAGAAAGAGGACAGTTGTTGGCGGTTGCTGGATCCACTGGAGCAGGCAAG
ACTTCACTTCTAATGATGATTATGGGAGAACTGGAGCCTTCAGAGGGTAAAATTAAGCAC
AGTGGAAGAATTTCATTCTGTTCTCAGTTTTCCTGGATTATGCCTGGCACCATTAAAGAA
AATATCATCTTTGGTGTTTCCTATGATGAATATAGATACAGAAGCGTCATCAAAGCATGC
CAACTAGAAGAAGACATCTCCAAGTTTGCAGAGAAAGACAATATAGTTCTTGGAGAAGGT
GGAATCACACTGAGTGGAGGTCAACGAGCAAGAATTTCTTTAGCAAGAGCAGTATACAAA
GATGCTGATTTGTATTTATTAGACTCTCCTTTTGGATACCTAGATGTTTTAACAGAAAAA
GAAATATTTGAAAGCTGTGTCTGTAAACTGATGGCTAACAAAACTAGGATTTTGGTCACT
TCTAAAATGGAACATTTAAAGAAAGCTGACAAAATATTAATTTTGCATGAAGGTAGCAGC
TATTTTTATGGGACATTTTCAGAACTCCAAAATCTACAGCCAGACTTTAGCTCAAAACTC
ATGGGAGAAGGGCATCATCACCATCACCACTGATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: End read sequencing of cDNA insert
Reference Sequence Annotations:
End on protein reference sequence 646
Start on protein reference sequence 388
Mutations:
387ins31, del405-436, N620H*

ins@388,31

Discrepancy:
None
Others:
Domain NBD1
Termini 388-646
5' Linker Sequence:
CATGGCCCT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  insect cells    Marker:  gentamicin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Protein_Purified
Find experimental protocols in 2935c
Recommended expression in: SF9
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pFASTBAC1-C-His-TOPO

pFASTBAC1-C-His-TOPO in advanced viewed

Synonyms: pFB1-C-His-TOPO, pFB1-C-His-TOPO_KW
Sequencing Primer: Forward:  pFB forward
Reverse:  pFB reverse
Description: Baculovirus vector, adds C-terminal 6xHis tag and TEV protease site; amp resistance; TOPO cloning.
Comments: None
Size (bp): 4763
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, baculovirus/insect cell expression, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start initiation ATG (Met, Ala, Leu) 4065 4078
TOPO recognition site TOPO TOPO cloning site 4069 4078
bacterial origin ori pUC-type bacterial origin of replication 1544 2226
poly-A signal polyA SV40 polyA signal sequence 4189 4380
primer pFB reverse reverse sequencing primer: ACAAATGTGGTATGGCTGATT 4150 4170
primer pFB foward forward sequencing primer:  TATTCCGGATTATTCATACC 3995 4014
promoter PH polyhedrin promoter for insect cell expression 3904 4032
selectable marker AmpR ampicillin resistance gene 787 1446
selectable marker GenR gentamycin resistance gene 3000 3533
ssDNA origin f1 ori f1 origin 19 325
tag His C-terminal 6xHis and stop 4079 4096
transposon fragment Tn7R Tn7R transposon site 2709 2933
transposon fragment Tn7L Tn7L transposon site 1466 12

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CFTR
Symbol Nomenclature : CFTR
SYNONYM : ABC35; ABCC7; CF; CFTR/MRP; MRP7; TNR-CFTR; dJ760C5.1
Designation : cAMP-dependent chloride channel|channel conductance-controlling ATPase|cystic fibrosis transmembrane conductance regulator
Full Nomenclature : cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
GENEID : 1080
Locus Tag : tcag7.78
GenBank Accession : NP_000483.3
HGNC : 1884
MIM : 602421
Vega : OTTHUMG00000023076
Target GenBank: NP_000483.3

 

Pathways

Reactome : ABC-family proteins mediated transport
Reactome : Transmembrane transport of small molecules

Protein

Domain : NBD1
Structural Annotation Wiki : TOPSAN
UniProt : P13569
HPRD : 03883

Clone

Cloning Information : 473959
HIP Master Clone ID : 395465
Original Clone ID : 2935c552KWg2h2

Experimental

TargetTrack - Experimental data : 2935c

Organism


TAX_ID : 9606
Species Specific ID: 1080

Genome

Chromosome : 7
Map Location : 7q31.2
Ensembl : ENSG00000001626

Reagents

Labome : CFTR-antibody