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Cftr (Mus musculus) in pSGX4 (His and Smt3-tagged bacterial expression vector)


Explanation of Terms

Gene: Gene Symbol:  Cftr
Gene Name:  cystic fibrosis transmembrane conductance regulator
Original Clone ID: 5833f2BSt1pi1
Keyword: Partial Protein, NBD2 domain
Species: Mus musculus
Type: cDNA
Vector Name: pSGX4               Format:  FUSION
Source: SGX Pharmaceuticals, Inc.
Description: This clone encodes the NBD2 domain with other intended mutations and corresponds to region 1189-1441 on full length protein NP_066388.1.
Comments: N-terminal 6-His and Smt3, cleavable, TOPO. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Discrepancy :
Yes / Unknown
Publications: None
Authors: SGX Pharmaceuticals, Inc.

Price:  Login for Pricing
No restriction
Special MTA: PSI/Smt3/SUMO


Insert sequence: 762nts        
Open reading frame : 1 to 762


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: End read sequencing of cDNA insert
Reference Sequence Annotations:
Start on protein reference sequence 1189
End on protein reference sequence 1441
Q1276E, H1398A, Q1407D, I1432D

Q1276E; H1398A; Q1407D; I1432D

Termini 1189-1441
Domain NBD2
5' Linker Sequence: None
3' Linker Sequence: None


 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 5833f
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pSGX4

pSGX4 in advanced viewed

Synonyms: pSGX-4, pSGX4_BS
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promotor, adds N-terminal 6xHis and smt-3 tags, cleavage site; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen. N-terminal 6xHis-tag followed by smt3 fusion protein. Smt3 fusion cloned into NheI and BamHI sites. TOPO adaptation at BamHI and XhoI sites.
Size (bp): 5611
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
TOPO recognition site TOPO TOPO cloning site 5435 5444
bacterial origin ColE ColE1 bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4984 5003
protease cleavage site ULP site ULP cleavage site (recognizes the tertiary structure of SUMO) 5140 5140
repressor protein gene LacI lacI cds 3515 4351
ribosome binding site RBS ribosome binding site (RBS) 5062 5068
selectable marker KanR kanamycin resistance gene 563 1375
tag Smt3 Smt3 tag 5140 5424
tag His 6xHis tag 5083 5100
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 termination sequence 5539 5585


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Gene Symbol : Cftr
Symbol Nomenclature : Cftr
SYNONYM : AW495489; Abcc7
Designation : ATP-binding cassette sub-family C member 7|ATP-binding cassette transporter sub-family C member 7|ATP-binding cassette, subfamily c, member 7|cAMP-dependent chloride channel|channel conductance-controlling ATPase|cystic fibrosis transmembrane conductance regulator homolog|cystic fibrosis transmembrane conductance regulator homolog; ATP-binding cassette, subfamily c, member 7
Full Nomenclature : cystic fibrosis transmembrane conductance regulator
GENEID : 12638
GenBank Accession : NP_066388.1
Mouse Genome Informatics : 88388
Vega : OTTMUSG00000024173
Target GenBank: NP_066388.1



Domain : NBD2
SMART domain : AAA : ATPases associated with a variety of cellular activities
Structural Annotation Wiki : TOPSAN
UniProt : P26361


Cloning Information : 474030
HIP Master Clone ID : 395536
Original Clone ID : 5833f2BSt1pi1


TargetTrack - Experimental data : 5833f


TAX_ID : 10090
Species Specific ID: 12638


Chromosome : 6
Map Location : 6 A3|6 8.1 cM
Ensembl : ENSMUSG00000041301


Labome : Cftr-antibody