CFTR (Fundulus heteroclitus) in pSGX4

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
5833b6BSt1pi1
Keyword:
Partial Protein, NBD2 domain
Species:
Fundulus heteroclitus
Type:
cDNA
Vector Name:
pSGX4
Format: 
FUSION
Source:
SGX Pharmaceuticals, Inc.
Description:
This clone encodes the NBD2 domain with other intended mutations and corresponds to region 1213-1463 on full length protein AAC41271.1.
Comments:
N-terminal 6-His and Smt3, cleavable, TOPO. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation / Discrepancy:
Yes / Unknown
Publications:
None
Authors:
SGX Pharmaceuticals, Inc.
FhCD00287436
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/Smt3/SUMO
Insert sequence: 756nts
Open reading frame: 1 to 756

ATTAATAACTTGTCTACGCACTCTTACTGGCCGAACCGTGGTCAACTGGAGGTGCAAGGC
CTGACTGTCAAATACACCGAGGCAGGCCGTGCGGTTCTGAACGATATCAGCTTTTCCGTC
GATGGTGGCCAAAGCGTTGGTCTGCTGGGTCGTACGGGTTCGGGTAAAAGCACCCTGCTG
TCTGCACTGTTGGGCCTGAACTACACCGAAGGTGAGATGTTTATTGATGGTGTGAGCTCC
TCCTCGATGCCGCTGCAGGCGTGGCGCAAAGCGTTCGGCGTTGTGCCTCAAAAAGTTTTC
ATTTTGACCGGCACTTTCCGCATGAATTTGGACCCGTATGGCCGCTACAGCGATGACGAA
CTGTGGCGTGTGGCCGAGGAAGTGGGTCTGAAATCCGTTATCGAGCAGTTCCCAGACAAG
TTGGACTTCGAACTGAAGGATGGTGGCAGCGTGCTGTCGAACGGTCACAAACAGTTGATG
TGCTTGGCGCGCTCGATCCTGTCCAAGGCTCGTATCCTGCTGCTGGACGAGCCGTCGTCG
TATCTGGATCCGATTACCTTGCAGGTCTTGCGTAAGACCCTGAAACAATCTTTCAGCGGT
TGTACCGTGATTCTGAGCGAACACAAAGTGGAGCCTCTGCTGGAGTGTGACAGCTTTCTG
ATGATCGAGAAGTCGTCCGTGAAATCCTACGATAGCATTCAGAAGCTGATGAACGAGATG
AGCCATCTGAAGCAAGCCATTTCCCCGGCCGATTAA

Protein sequence predicted based on nucleotide sequence

INNLSTHSYWPNRGQLEVQGLTVKYTEAGRAVLNDISFSVDGGQSVGLLGRTGSGKSTLL
SALLGLNYTEGEMFIDGVSSSSMPLQAWRKAFGVVPQKVFILTGTFRMNLDPYGRYSDDE
LWRVAEEVGLKSVIEQFPDKLDFELKDGGSVLSNGHKQLMCLARSILSKARILLLDEPSS
YLDPITLQVLRKTLKQSFSGCTVILSEHKVEPLLECDSFLMIEKSSVKSYDSIQKLMNEM
SHLKQAISPAD*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Reference Sequence Annotations:
Start on protein reference sequence 1213
End on protein reference sequence 1463
Mutations:
Q1429D

Discrepancy:
None
Others:
Termini 1213-1463
Domain NBD2
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Tested
SolubleProtein: Not_Tested
ProteinPurified: Protein_Purified
Find experimental protocols in 5833b
Recommended expression in:
Not Applicable