Gene:
Gene Symbol:
LOC100136364
Gene Name:
cystic fibrosis transmembrane conductance regulator I
Original Clone ID:
2935a3BCt3b1
Keyword:
Partial Protein, NBD1 domain
Species:
Salmo salar
Type:
cDNA
Vector Name:
pSGX3
Format:
FUSION
Source:
SGX Pharmaceuticals, Inc.
Description:
This clone encodes the NBD1 domain and corresponds to region 390-678 on full length protein AAK07405.1.
Comments:
C-terminal 6-His, TOPO. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation / Discrepancy:
No / Unknown
Publications:
None
Authors:
SGX Pharmaceuticals, Inc.
SsCD00287531
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 894nts
Open reading frame: 1 to 894
ACCACAGAAATTGAACTCGTGAACGTGACGGCCAGCTGGGATGAGGGCATTGGCGAACTT
TTCGAAAAAATCAAACAGGAAAATAAAGCGAACGGCCAGCCAAACGGCGACGCGGGTCTG
TTTTTTACCAACTTGTACGTCACCCCGGTGCTGAAAAATATTAGCCTTTACCTCGAGAAA
GGGCAGATGCTGGCGGTGGCAGGGTCAACGGGCAGCGGCAAATCCTCCTTACTGATGATG
ATTCTGGGTGAGCTTGTGCCTTCCGAGGGCAAGATTCGTCATTCGGGCCGGATCAGCTTT
TCCCCGCAGACTTCGTGGATTATGCCAGGCACCATTCGCGATAACATCCTTTTTGGTCTT
ACGTATGATGAATTTCGTTACACCAGTGTCATTAAGGCCTGCCAGCTGGAAGAGGATCTG
GCCTTACTGCCGGAAAAAGACAAGACCCCGCTTATGGAAGGTGGCGTCACGTTGAGCGGC
GGTCAACGCGCGCGCATCGGTCTCGCTCGTGCTGTGTACAAAGACGCAGACCTGTATCTG
CTCGACAGTCCATTCACGCATCTGGATATTGTTACCGAAAAGGAAATCTTTGAACGCTGT
CTGTGCAAACTCCTTTCTAGCAAAACGCGTATCGTCGTCACCTCGAAACTGGAACACCTG
AAACGCGCGGACAAGATCCTGTTACTGCACAACGGGGTCTGCTATTTTTACGGTTCTTTC
TCGGAACTGCAGGCCAAACGCCCGGATTTTTCTTCATTACTGTTGGGGTTAGAAGCCTAC
GACAACATCAAAGCAGAACGCCGTTCATCCATTTTGACCGAAACGCTGCGCCGCGTCTCC
ATTGACGAATCAACCGTATTCCGTGGTGAAGGGCATCATCACCATCACCACTGA
Protein sequence predicted based on nucleotide sequence
TTEIELVNVTASWDEGIGELFEKIKQENKANGQPNGDAGLFFTNLYVTPVLKNISLYLEK GQMLAVAGSTGSGKSSLLMMILGELVPSEGKIRHSGRISFSPQTSWIMPGTIRDNILFGL TYDEFRYTSVIKACQLEEDLALLPEKDKTPLMEGGVTLSGGQRARIGLARAVYKDADLYL LDSPFTHLDIVTEKEIFERCLCKLLSSKTRIVVTSKLEHLKRADKILLLHNGVCYFYGSF SELQAKRPDFSSLLLGLEAYDNIKAERRSSILTETLRRVSIDESTVFRGEGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Reference Sequence Annotations:
Start on protein reference sequence
390
End on protein reference sequence
678
Mutations:
None
Discrepancy:
None
Others:
Termini
390-678
Domain NBD1
Domain NBD1
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in 2935a
Recommended expression in:
Not Applicable
Vector Name:
pSGX3
Synonyms:
pSGX-3, pSGX3_BC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation start (Met, Ser, Leu)
5072
5079
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4983
5003
repressor protein gene
LacI
LacI CDS
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5086
5103
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5231
5307
