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Detailed Plasmid Information

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Explanation of Terms


Gene: Gene Symbol:  LOC100136364
Gene Name:  cystic fibrosis transmembrane conductance regulator I
Sequence             
Original Clone ID: 5806a33BSt4p1
Keyword: Partial Protein, NBD1 domain
Species: Salmo salar
Type: cDNA
Vector Name: pSGX4               Format:  FUSION
Source: SGX Pharmaceuticals, Inc.
Description: This clone encodes the NBD1 domain and corresponds to region 417-662 on full length protein AAK07405.1.
Comments: N-terminal 6-His and Smt3, cleavable, TOPO. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation/
Discrepancy :
No / Unknown
Publications: None
Authors: SGX Pharmaceuticals, Inc.
SsCD00287537

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AVAILABLE
No restriction
Special MTA: PSI/Smt3/SUMO

 

Insert sequence: 741nts        
Open reading frame : 1 to 741

AATAAAGCGAACGGCCAGCCAAACGGCGACGCGGGTCTGTTTTTTACCAACTTGTACGTC
ACCCCGGTGCTGAAAAATATTAGCCTTTACCTCGAGAAAGGGCAGATGCTGGCGGTGGCA
GGGTCAACGGGCAGCGGCAAATCCTCCTTACTGATGATGATTCTGGGTGAGCTTGTGCCT
TCCGAGGGCAAGATTCGTCATTCGGGCCGGATCAGCTTTTCCCCGCAGACTTCGTGGATT
ATGCCAGGCACCATTCGCGATAACATCCTTTTTGGTCTTACGTATGATGAATTTCGTTAC
ACCAGTGTCATTAAGGCCTGCCAGCTGGAAGAGGATCTGGCCTTACTGCCGGAAAAAGAC
AAGACCCCGCTTATGGAAGGTGGCGTCACGTTGAGCGGCGGTCAACGCGCGCGCATCGGT
CTCGCTCGTGCTGTGTACAAAGACGCAGACCTGTATCTGCTCGACAGTCCATTCACGCAT
CTGGATATTGTTACCGAAAAGGAAATCTTTGAACGCTGTCTGTGCAAACTCCTTTCTAGC
AAAACGCGTATCGTCGTCACCTCGAAACTGGAACACCTGAAACGCGCGGACAAGATCCTG
TTACTGCACAACGGGGTCTGCTATTTTTACGGTTCTTTCTCGGAACTGCAGGCCAAACGC
CCGGATTTTTCTTCATTACTGTTGGGGTTAGAAGCCTACGACAACATCAAAGCAGAACGC
CGTTCATCCATTTTGACCTAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: End read sequencing of cDNA insert
Reference Sequence Annotations:
Start on protein reference sequence 417
End on protein reference sequence 662
Mutations:
None
Discrepancy:
None
Others:
Termini 417-662
Domain NBD1
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in 5806a
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pSGX4

pSGX4 in advanced viewed

Synonyms: pSGX-4, pSGX4_BS
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promotor, adds N-terminal 6xHis and smt-3 tags, cleavage site; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen. N-terminal 6xHis-tag followed by smt3 fusion protein. Smt3 fusion cloned into NheI and BamHI sites. TOPO adaptation at BamHI and XhoI sites.
Size (bp): 5611
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
TOPO recognition site TOPO TOPO cloning site 5435 5444
bacterial origin ColE ColE1 bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4984 5003
protease cleavage site ULP site ULP cleavage site (recognizes the tertiary structure of SUMO) 5140 5140
repressor protein gene LacI lacI cds 3515 4351
ribosome binding site RBS ribosome binding site (RBS) 5062 5068
selectable marker KanR kanamycin resistance gene 563 1375
tag Smt3 Smt3 tag 5140 5424
tag His 6xHis tag 5083 5100
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 termination sequence 5539 5585

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : LOC100136364
GENEID : 100136364
GenBank Accession : AAK07405.1
Target GenBank: AAK07405.1

 

Protein

Domain : NBD1
SMART domain : AAA : ATPases associated with a variety of cellular activities
Structural Annotation Wiki : TOPSAN
UniProt : Q9W750

Clone

Cloning Information : 474288
HIP Master Clone ID : 395794
Original Clone ID : 5806a33BSt4p1

Experimental

TargetTrack - Experimental data : 5806a

Organism


TAX_ID : 8030
Species Specific ID: 100136364

Reagents

Labome : LOC100136364-cDNA