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Detailed Vector Information: pMCSG29

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG29              
Source: Midwest Center for Structural Genomics
Description: Bacterial expression vector with T7 promoter, C-terminal 10xHis tag, TEV site, C-terminal MBP with TVMV site; ampicillin resistance in bacterial; ligation independent cloning (LIC)
Comments: None
Publications: PMID: 20213425
Title: Cleavable C-terminal His-tag vectors for structure determination.
Authors:
EvNO00287601

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pMCSG29

pMCSG29 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter, C-terminal 10xHis tag, TEV site, C-terminal MBP with TVMV site; ampicillin resistance in bacterial; ligation independent cloning (LIC)
Comments: For more information about MCSG vectors visit their website at: http://bioinformatics.anl.gov/mcsg/technologies/vectors.html
Size (bp): 6389
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: PSI
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
Publications: PMID: 20213425
Title: Cleavable C-terminal His-tag vectors for structure determination.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin of replication 4383 4383
promoter T7 T7 promoter 1463 1479
protease cleavage site TEV TEV protease cleavage site 1368 1388
protease cleavage site TVMV TVMV protease cleavage site 1311 1331
recombination site LIC Ligation Independent Cloning (LIC) recombination site 1382 1400
repressor binding site LacI LacI coding region 1993 2952
repressor binding site lac operator LacO fragment 3363 3385
selectable marker AmpR Ampicillin resistance gene 5141 6001
tag His C-terminal 10xHis Tag 1332 1361
tag MBP C-terminal Maltose binding protein (MBP) tag 204 1310
trxn termination sequence T7 term T7 transcription termination sequence 26 72

 

Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Other Link: SBKB Technology Portal
Species Specific ID: None
Target GenBank: None