| Gene: |
Gene Symbol:
No insert Gene Name: No insert |
|---|---|
| Original Clone ID: | None |
| Type: | No insert |
| Vector Name: | pMCSG29 |
| Source: | Midwest Center for Structural Genomics |
| Description: | Bacterial expression vector with T7 promoter, C-terminal 10xHis tag, TEV site, C-terminal MBP with TVMV site; ampicillin resistance in bacterial; ligation independent cloning (LIC) |
| Comments: | None |
| Publications: |
PMID: 20213425 Title: Cleavable C-terminal His-tag vectors for structure determination. |
| Authors: |
|
EvNO00287601 Price: Login for Pricing AVAILABLE No restriction Special MTA: PSI |
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
ampicillin Bacterial Selection Condition: 100 ug/mL ampicillin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Commonly used conditions for ampicillin resistant plasmid clones. |
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| Vector Name: | pMCSG29 |
pMCSG29 in advanced viewed |
| Synonyms: | None | |
| Sequencing Primer: | Forward:
T7
Reverse: T7 terminator |
|
| Description: | Bacterial expression vector with T7 promoter, C-terminal 10xHis tag, TEV site, C-terminal MBP with TVMV site; ampicillin resistance in bacterial; ligation independent cloning (LIC) | |
| Comments: | For more information about MCSG vectors visit their website at: http://bioinformatics.anl.gov/mcsg/technologies/vectors.html | |
| Size (bp): | 6389 | |
| Parent Vector: | None | |
| Properties: | bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker | |
| Author Name: |
PSI Midwest Center for Structural Genomics Midwest Center for Structural Genomics |
|
| Publications: |
PMID:
20213425
Title: Cleavable C-terminal His-tag vectors for structure determination. |
|
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| bacterial origin | pBR322 origin | pBR322 bacterial origin of replication | 4383 | 4383 |
| promoter | T7 | T7 promoter | 1463 | 1479 |
| protease cleavage site | TEV | TEV protease cleavage site | 1368 | 1388 |
| protease cleavage site | TVMV | TVMV protease cleavage site | 1311 | 1331 |
| recombination site | LIC | Ligation Independent Cloning (LIC) recombination site | 1382 | 1400 |
| repressor binding site | LacI | LacI coding region | 1993 | 2952 |
| repressor binding site | lac operator | LacO fragment | 3363 | 3385 |
| selectable marker | AmpR | Ampicillin resistance gene | 5141 | 6001 |
| tag | His | C-terminal 10xHis Tag | 1332 | 1361 |
| tag | MBP | C-terminal Maltose binding protein (MBP) tag | 204 | 1310 |
| trxn termination sequence | T7 term | T7 transcription termination sequence | 26 | 72 |
| Other Link: |
MCSG Cloning Information |
|---|---|
| Other Link: |
MCSG Vector Information |
| Other Link: |
SBKB Technology Portal |
| Species Specific ID: | None |
| Target GenBank: | None |
