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NSMCE2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  NSMCE2
Gene Name:  non-SMC element 2, MMS21 homolog (S. cerevisiae)
Sequence             
Original Clone ID: 1750
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00287670

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 744nts         Open reading frame : 1 to 744
Reference Sequence Alignment

ATGCCAGGACGTTCCAGTTCAAATTCAGGTTCAACTGGTTTCATCTCCTTCAGTGGTGTA
GAGTCTGCTCTCTCCTCCTTGAAAAACTTCCAAGCCTGTATCAACTCTGGTATGGACACA
GCTTCTAGTGTTGCTTTGGATCTTGTGGAAAGTCAGACTGAAGTGAGTAGTGAATATAGT
ATGGACAAGGCAATGGTTGAATTTGCTACATTGGATCGGCAACTAAACCATTATGTAAAG
GCTGTTCAATCTACAATAAATCATGTGAAAGAAGAACGTCCAGAAAAAATACCAGATTTA
AAATTATTGGTAGAGAAGAAATTTTTGGCTTTACAGAGCAAGAATTCTGATGCAGACTTT
CAAAATAATGAAAAATTTGTACAGTTTAAACAACAGCTGAAAGAACTAAAGAAGCAATGT
GGTCTTCAAGCTGACAGAGAAGCTGACGGAACAGAAGGAGTGGATGAAGATATAATTGTG
ACCCAAAGTCAGACCAACTTCACCTGCCCCATTACAAAGGAGGAAATGAAGAAGCCAGTG
AAAAATAAAGTGTGTGGCCACACCTATGAAGAGGACGCCATTGTTCGCATGATTGAGTCC
AGGCAAAAGCGGAAGAAAAAGGCCTATTGCCCTCAAATTGGCTGTAGCCACACGGATATA
AGAAAGTCAGATCTTATCCAGGATGAAGCACTTAGAAGGGCAATTGAGAACCATAACAAG
AAAAGACATCGTCATTCCGAGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 971
Start on reference sequence 228
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGTTGGC

3' Linker Sequence:
5p (goi-stop)CCAACTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NSMCE2
Symbol Nomenclature : NSMCE2
SYNONYM : C8orf36; MMS21; NSE2; ZMIZ7
Designation : E3 SUMO-protein ligase NSE2|MMS21 homolog|hMMS21|methyl methanesulfonate sensitivity gene 21|non-SMC element 2 homolog|non-structural maintenance of chromosomes element 2 homolog|zinc finger, MIZ-type containing 7
Full Nomenclature : non-SMC element 2, MMS21 homolog (S. cerevisiae)
GENEID : 286053
HGNC : 26513
Vega : OTTHUMG00000164993
Target GenBank: BC032797.1

 

Reference Sequence Alignment

  

HsCD00287670        ATGCCAGGACGTTCCAGTTCAAATTCAGGTTCAACTGGTTTCATCTCCTTCAGTGGTGTA
NM_173685.2         ATGCCAGGACGTTCCAGTTCAAATTCAGGTTCAACTGGTTTCATCTCCTTCAGTGGTGTA
XM_005250875.1      ATGCCAGGACGTTCCAGTTCAAATTCAGGTTCAACTGGTTTCATCTCCTTCAGTGGTGTA
                    ************************************************************

HsCD00287670        GAGTCTGCTCTCTCCTCCTTGAAAAACTTCCAAGCCTGTATCAACTCTGGTATGGACACA
NM_173685.2         GAGTCTGCTCTCTCCTCCTTGAAAAACTTCCAAGCCTGTATCAACTCTGGTATGGACACA
XM_005250875.1      GAGTCTGCTCTCTCCTCCTTGAAAAACTTCCAAGCCTGTATCAACTCTGGTATGGACACA
                    ************************************************************

HsCD00287670        GCTTCTAGTGTTGCTTTGGATCTTGTGGAAAGTCAGACTGAAGTGAGTAGTGAATATAGT
NM_173685.2         GCTTCTAGTGTTGCTTTGGATCTTGTGGAAAGTCAGACTGAAGTGAGTAGTGAATATAGT
XM_005250875.1      GCTTCTAGTGTTGCTTTGGATCTTGTGGAAAGTCAGACTGAAGTGAGTAGTGAATATAGT
                    ************************************************************

HsCD00287670        ATGGACAAGGCAATGGTTGAATTTGCTACATTGGATCGGCAACTAAACCATTATGTAAAG
NM_173685.2         ATGGACAAGGCAATGGTTGAATTTGCTACATTGGATCGGCAACTAAACCATTATGTAAAG
XM_005250875.1      ATGGACAAGGCAATGGTTGAATTTGCTACATTGGATCGGCAACTAAACCATTATGTAAAG
                    ************************************************************

HsCD00287670        GCTGTTCAATCTACAATAAATCATGTGAAAGAAGAACGTCCAGAAAAAATACCAGATTTA
NM_173685.2         GCTGTTCAATCTACAATAAATCATGTGAAAGAAGAACGTCCAGAAAAAATACCAGATTTA
XM_005250875.1      GCTGTTCAATCTACAATAAATCATGTGAAAGAAGAACGTCCAGAAAAAATACCAGATTTA
                    ************************************************************

HsCD00287670        AAATTATTGGTAGAGAAGAAATTTTTGGCTTTACAGAGCAAGAATTCTGATGCAGACTTT
NM_173685.2         AAATTATTGGTAGAGAAGAAATTTTTGGCTTTACAGAGCAAGAATTCTGATGCAGACTTT
XM_005250875.1      AAATTATTGGTAGAGAAGAAATTTTTGGCTTTACAGAGCAAGAATTCTGATGCAGACTTT
                    ************************************************************

HsCD00287670        CAAAATAATGAAAAATTTGTACAGTTTAAACAACAGCTGAAAGAACTAAAGAAGCAATGT
NM_173685.2         CAAAATAATGAAAAATTTGTACAGTTTAAACAACAGCTGAAAGAACTAAAGAAGCAATGT
XM_005250875.1      CAAAATAATGAAAAATTTGTACAGTTTAAACAACAGCTGAAAGAACTAAAGAAGCAATGT
                    ************************************************************

HsCD00287670        GGTCTTCAAGCTGACAGAGAAGCTGACGGAACAGAAGGAGTGGATGAAGATATAATTGTG
NM_173685.2         GGTCTTCAAGCTGACAGAGAAGCTGACGGAACAGAAGGAGTGGATGAAGATATAATTGTG
XM_005250875.1      GGTCTTCAAGCTGACAGAGAAGCTGACGGAACAGAAGGAGTGGATGAAGATATAATTGTG
                    ************************************************************

HsCD00287670        ACCCAAAGTCAGACCAACTTCACCTGCCCCATTACAAAGGAGGAAATGAAGAAGCCAGTG
NM_173685.2         ACCCAAAGTCAGACCAACTTCACCTGCCCCATTACAAAGGAGGAAATGAAGAAGCCAGTG
XM_005250875.1      ACCCAAAGTCAGACCAACTTCACCTGCCCCATTACAAAGGAGGAAATGAAGAAGCCAGTG
                    ************************************************************

HsCD00287670        AAAAATAAAGTGTGTGGCCACACCTATGAAGAGGACGCCATTGTTCGCATGATTGAGTCC
NM_173685.2         AAAAATAAAGTGTGTGGCCACACCTATGAAGAGGACGCCATTGTTCGCATGATTGAGTCC
XM_005250875.1      AAAAATAAAGTGTGTGGCCACACCTATGAAGAGGACGCCATTGTTCGCATGATTGAGTCC
                    ************************************************************

HsCD00287670        AGGCAAAAGCGGAAGAAAAAGGCCTATTGCCCTCAAATTGGCTGTAGCCACACGGATATA
NM_173685.2         AGGCAAAAGCGGAAGAAAAAGGCCTATTGCCCTCAAATTGGCTGTAGCCACACGGATATA
XM_005250875.1      AGGCAAAAGCGGAAGAAAAAGGCCTATTGCCCTCAAATTGGCTGTAGCCACACGGATATA
                    ************************************************************

HsCD00287670        AGAAAGTCAGATCTTATCCAGGATGAAGCACTTAGAAGGGCAATTGAGAACCATAACAAG
NM_173685.2         AGAAAGTCAGATCTTATCCAGGATGAAGCACTTAGAAGGGCAATTGAGAACCATAACAAG
XM_005250875.1      AGAAAGTCAGATCTTATCCAGGATGAAGCACTTAGAAGGGCAATTGAGAACCATAACAAG
                    ************************************************************

HsCD00287670        AAAAGACATCGTCATTCCGAGTAC
NM_173685.2         AAAAGACATCGTCATTCCGAGTAG
XM_005250875.1      AAAAGACATCGTCATTCCGAGTAG
                    *********************** 

Protein

SMART domain : Ubox : Modified RING finger domain
UniProt : Q96MF7
HPRD : 08131

Clone

Cloning Information : 470907
HIP Master Clone ID : 393825
Original Clone ID : 1750

Organism


TAX_ID : 9606
Species Specific ID: 286053

Genome

Chromosome : 8
Map Location : 8q24.13
Ensembl : ENSG00000156831

Reagents

Labome : NSE2-antibody