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KLHDC8A (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHDC8A
Gene Name:  kelch domain containing 8A
Sequence             
Original Clone ID: 12141
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00287767

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1053nts         Open reading frame : 1 to 1053
Reference Sequence Alignment

ATGGAGGTGCCTAACGTCAAGGACTTCCAGTGGAAGCGCCTGGCGCCACTGCCCAGCCGC
CGGGTCTACTGCTCCCTGCTGGAGACCGGGGGCCAGGTCTATGCCATCGGGGGATGTGAC
GACAACGGCGTCCCCATGGACTGCTTCGAGGTCTACTCCCCGGAGGCCGACCAGTGGACC
GCCTTGCCCCGGCTGCCCACAGCACGGGCGGGGGTGGCCGTCACCGCCCTGGGGAAGCGG
ATCATGGTGATTGGGGGCGTGGGCACCAATCAGCTGCCCCTGAAGGTCGTGGAGATGTAC
AACATCGATGAGGGCAAGTGGAAGAAGAGGAGCATGCTGCGTGAGGCCGCCATGGGCATT
TCTGTCACGGCCAAAGATTACCGAGTATATGCGGCAGGCGGGATGGGCCTGGACCTACGT
CCACACAACCACCTCCAACACTATGACATGCTGAAGGACATGTGGGTGTCCCTAGCACAC
ATGCCCACCCCGAGATATGCTGCCACCTCCTTCCTCCGAGGCTCCAAGATCTACGTGCTG
GGGGGACGACAGTCCAAGTACGCGGTCAACGCTTTCGAGGTCTTTGACATCGAGACTCGC
TCCTGGACCAAGTTTCCCAACATTCCCTATAAGCGGGCCTTCTCCAGCTTTGTGACCCTG
GACAACCACTTGTACAGCCTAGGAGGCCTGCGGCAAGGTCGCCTCTACCGGCAGCCAAAG
TTCCTGCGGACGATGGACGTGTTCGACATGGAACAGGGGGGATGGCTGAAGATGGAACGA
TCGTTCTTCCTCAAGAAGCGGCGGGCAGATTTTGTGGCTGGCTCTCTGAGTGGACGGGTC
ATAGTGGCTGGGGGACTTGGGAATCAACCCACTGTCCTGGAGACGGCGGAAGCATTCCAC
CCAGGGAAGAACAAATGGGAGATCCTCCCTGCCATGCCCACACCCCGCTGTGCCTGCTCC
AGCATAGTCGTCAAGAACTGCCTCCTCGCCGTGGGAGGTGTCAACCAGGGTCTGAGTGAC
GCAGTGGAGGCCCTGTGTGTCTCTGACTCCTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1291
Start on reference sequence 239
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGTTGGC

3' Linker Sequence:
5p (goi-stop)CCAACTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHDC8A
Symbol Nomenclature : KLHDC8A
Designation : S-delta-E1|kelch domain-containing protein 8A|substitute for delta-EGFR expression 1
Full Nomenclature : kelch domain containing 8A
GENEID : 55220
HGNC : 25573
MIM : 614503
Vega : OTTHUMG00000037199
Target GenBank: BC036076.1

 

Reference Sequence Alignment

  

HsCD00287767        ATGGAGGTGCCTAACGTCAAGGACTTCCAGTGGAAGCGCCTGGCGCCACTGCCCAGCCGC
NM_018203.2         ATGGAGGTGCCTAACGTCAAGGACTTCCAGTGGAAGCGCCTGGCGCCACTGCCCAGCCGC
NM_001271863.1      ATGGAGGTGCCTAACGTCAAGGACTTCCAGTGGAAGCGCCTGGCGCCACTGCCCAGCCGC
NM_001271864.1      ATGGAGGTGCCTAACGTCAAGGACTTCCAGTGGAAGCGCCTGGCGCCACTGCCCAGCCGC
NM_001271865.1      ATGGAGGTGCCTAACGTCAAGGACTTCCAGTGGAAGCGCCTGGCGCCACTGCCCAGCCGC
                    ************************************************************

HsCD00287767        CGGGTCTACTGCTCCCTGCTGGAGACCGGGGGCCAGGTCTATGCCATCGGGGGATGTGAC
NM_018203.2         CGGGTCTACTGCTCCCTGCTGGAGACCGGGGGCCAGGTCTATGCCATCGGGGGATGTGAC
NM_001271863.1      CGGGTCTACTGCTCCCTGCTGGAGACCGGGGGCCAGGTCTATGCCATCGGGGGATGTGAC
NM_001271864.1      CGGGTCTACTGCTCCCTGCTGGAGACCGGGGGCCAGGTCTATGCCATCGGGGGATGTGAC
NM_001271865.1      CGGGTCTACTGCTCCCTGCTGGAGACCGGGGGCCAGGTCTATGCCATCGGGGGATGTGAC
                    ************************************************************

HsCD00287767        GACAACGGCGTCCCCATGGACTGCTTCGAGGTCTACTCCCCGGAGGCCGACCAGTGGACC
NM_018203.2         GACAACGGCGTCCCCATGGACTGCTTCGAGGTCTACTCCCCGGAGGCCGACCAGTGGACC
NM_001271863.1      GACAACGGCGTCCCCATGGACTGCTTCGAGGTCTACTCCCCGGAGGCCGACCAGTGGACC
NM_001271864.1      GACAACGGCGTCCCCATGGACTGCTTCGAGGTCTACTCCCCGGAGGCCGACCAGTGGACC
NM_001271865.1      GACAACGGCGTCCCCATGGACTGCTTCGAGGTCTACTCCCCGGAGGCCGACCAGTGGACC
                    ************************************************************

HsCD00287767        GCCTTGCCCCGGCTGCCCACAGCACGGGCGGGGGTGGCCGTCACCGCCCTGGGGAAGCGG
NM_018203.2         GCCTTGCCCCGGCTGCCCACAGCCCGGGCGGGGGTGGCCGTCACCGCCCTGGGGAAGCGG
NM_001271863.1      GCCTTGCCCCGGCTGCCCACAGCCCGGGCGGGGGTGGCCGTCACCGCCCTGGGGAAGCGG
NM_001271864.1      GCCTTGCCCCGGCTGCCCACAGCCCGGGCGGGGGTGGCCGTCACCGCCCTGGGGAAGCGG
NM_001271865.1      GCCTTGCCCCGGCTGCCCACAGCCCGGGCGGGGGTGGCCGTCACCGCCCTGGGGAAGCGG
                    ***********************.************************************

HsCD00287767        ATCATGGTGATTGGGGGCGTGGGCACCAATCAGCTGCCCCTGAAGGTCGTGGAGATGTAC
NM_018203.2         ATCATGGTGATTGGGGGCGTGGGCACCAATCAGCTGCCCCTGAAGGTCGTGGAGATGTAC
NM_001271863.1      ATCATGGTGATTGGGGGCGTGGGCACCAATCAGCTGCCCCTGAAGGTCGTGGAGATGTAC
NM_001271864.1      ATCATGGTGATTGGGGGCGTGGGCACCAATCAGCTGCCCCTGAAGGTCGTGGAGATGTAC
NM_001271865.1      ATCATGGTGATTGGGGGCGTGGGCACCAATCAGCTGCCCCTGAAGGTCGTGGAGATGTAC
                    ************************************************************

HsCD00287767        AACATCGATGAGGGCAAGTGGAAGAAGAGGAGCATGCTGCGTGAGGCCGCCATGGGCATT
NM_018203.2         AACATCGATGAGGGCAAGTGGAAGAAGAGGAGCATGCTGCGTGAGGCCGCCATGGGCATT
NM_001271863.1      AACATCGATGAGGGCAAGTGGAAGAAGAGGAGCATGCTGCGTGAGGCCGCCATGGGCATT
NM_001271864.1      AACATCGATGAGGGCAAGTGGAAGAAGAGGAGCATGCTGCGTGAGGCCGCCATGGGCATT
NM_001271865.1      AACATCGATGAGGGCAAGTGGAAGAAGAGGAGCATGCTGCGTGAGGCCGCCATGGGCATT
                    ************************************************************

HsCD00287767        TCTGTCACGGCCAAAGATTACCGAGTATATGCGGCAGGCGGGATGGGCCTGGACCTACGT
NM_018203.2         TCTGTCACGGCCAAAGATTACCGAGTATATGCGGCAGGCGGGATGGGCCTGGACCTACGT
NM_001271863.1      TCTGTCACGGCCAAAGATTACCGAGTATATGCGGCAGGCGGGATGGGCCTGGACCTACGT
NM_001271864.1      TCTGTCACGGCCAAAGATTACCGAGTATATGCGGCAGGCGGGATGGGCCTGGACCTACGT
NM_001271865.1      TCTGTCACGGCCAAAGATTACCGAGTATATGCGGCAGGCGGGATGGGCCTGGACCTACGT
                    ************************************************************

HsCD00287767        CCACACAACCACCTCCAACACTATGACATGCTGAAGGACATGTGGGTGTCCCTAGCACAC
NM_018203.2         CCACACAACCACCTCCAACACTATGACATGCTGAAGGACATGTGGGTGTCCCTAGCACCC
NM_001271863.1      CCACACAACCACCTCCAACACTATGACATGCTGAAGGACATGTGGGTGTCCCTAGCACCC
NM_001271864.1      CCACACAACCACCTCCAACACTATGACATGCTGAAGGACATGTGGGTGTCCCTAGCACCC
NM_001271865.1      CCACACAACCACCTCCAACACTATGACATGCTGAAGGACATGTGGGTGTCCCTAGCACCC
                    **********************************************************.*

HsCD00287767        ATGCCCACCCCGAGATATGCTGCCACCTCCTTCCTCCGAGGCTCCAAGATCTACGTGCTG
NM_018203.2         ATGCCCACCCCGAGATATGCTGCCACCTCCTTCCTCCGAGGCTCCAAGATCTACGTGCTG
NM_001271863.1      ATGCCCACCCCGAGATATGCTGCCACCTCCTTCCTCCGAGGCTCCAAGATCTACGTGCTG
NM_001271864.1      ATGCCCACCCCGAGATATGCTGCCACCTCCTTCCTCCGAGGCTCCAAGATCTACGTGCTG
NM_001271865.1      ATGCCCACCCCGAGATATGCTGCCACCTCCTTCCTCCGAGGCTCCAAGATCTACGTGCTG
                    ************************************************************

HsCD00287767        GGGGGACGACAGTCCAAGTACGCGGTCAACGCTTTCGAGGTCTTTGACATCGAGACTCGC
NM_018203.2         GGGGGACGACAGTCCAAGTACGCGGTCAACGCTTTCGAGGTCTTTGACATCGAGACTCGC
NM_001271863.1      GGGGGACGACAGTCCAAGTACGCGGTCAACGCTTTCGAGGTCTTTGACATCGAGACTCGC
NM_001271864.1      GGGGGACGACAGTCCAAGTACGCGGTCAACGCTTTCGAGGTCTTTGACATCGAGACTCGC
NM_001271865.1      GGGGGACGACAGTCCAAGTACGCGGTCAACGCTTTCGAGGTCTTTGACATCGAGACTCGC
                    ************************************************************

HsCD00287767        TCCTGGACCAAGTTTCCCAACATTCCCTATAAGCGGGCCTTCTCCAGCTTTGTGACCCTG
NM_018203.2         TCCTGGACCAAGTTTCCCAACATTCCCTATAAGCGGGCCTTCTCCAGCTTTGTGACCCTG
NM_001271863.1      TCCTGGACCAAGTTTCCCAACATTCCCTATAAGCGGGCCTTCTCCAGCTTTGTGACCCTG
NM_001271864.1      TCCTGGACCAAGTTTCCCAACATTCCCTATAAGCGGGCCTTCTCCAGCTTTGTGACCCTG
NM_001271865.1      TCCTGGACCAAGTTTCCCAACATTCCCTATAAGCGGGCCTTCTCCAGCTTTGTGACCCTG
                    ************************************************************

HsCD00287767        GACAACCACTTGTACAGCCTAGGAGGCCTGCGGCAAGGTCGCCTCTACCGGCAGCCAAAG
NM_018203.2         GACAACCACTTGTACAGCCTAGGAGGCCTGCGGCAAGGTCGCCTCTACCGGCAGCCCAAG
NM_001271863.1      GACAACCACTTGTACAGCCTAGGAGGCCTGCGGCAAGGTCGCCTCTACCGGCAGCCCAAG
NM_001271864.1      GACAACCACTTGTACAGCCTAGGAGGCCTGCGGCAAGGTCGCCTCTACCGGCAGCCCAAG
NM_001271865.1      GACAACCACTTGTACAGCCTAGGAGGCCTGCGGCAAGGTCGCCTCTACCGGCAGCCCAAG
                    ********************************************************.***

HsCD00287767        TTCCTGCGGACGATGGACGTGTTCGACATGGAACAGGGGGGATGGCTGAAGATGGAACGA
NM_018203.2         TTCCTGCGGACGATGGACGTGTTCGACATGGAACAGGGGGGATGGCTGAAGATGGAACGA
NM_001271863.1      TTCCTGCGGACGATGGACGTGTTCGACATGGAACAGGGGGGATGGCTGAAGATGGAACGA
NM_001271864.1      TTCCTGCGGACGATGGACGTGTTCGACATGGAACAGGGGGGATGGCTGAAGATGGAACGA
NM_001271865.1      TTCCTGCGGACGATGGACGTGTTCGACATGGAACAGGGGGGATGGCTGAAGATGGAACGA
                    ************************************************************

HsCD00287767        TCGTTCTTCCTCAAGAAGCGGCGGGCAGATTTTGTGGCTGGCTCTCTGAGTGGACGGGTC
NM_018203.2         TCGTTCTTCCTCAAGAAGCGGCGGGCAGATTTTGTGGCTGGCTCTCTGAGTGGACGGGTC
NM_001271863.1      TCGTTCTTCCTCAAGAAGCGGCGGGCAGATTTTGTGGCTGGCTCTCTGAGTGGACGGGTC
NM_001271864.1      TCGTTCTTCCTCAAGAAGCGGCGGGCAGATTTTGTGGCTGGCTCTCTGAGTGGACGGGTC
NM_001271865.1      TCGTTCTTCCTCAAGAAGCGGCGGGCAGATTTTGTGGCTGGCTCTCTGAGTGGACGGGTC
                    ************************************************************

HsCD00287767        ATAGTGGCTGGGGGACTTGGGAATCAACCCACTGTCCTGGAGACGGCGGAAGCATTCCAC
NM_018203.2         ATAGTGGCTGGGGGACTTGGGAATCAACCCACTGTCCTGGAGACGGCGGAAGCATTCCAC
NM_001271863.1      ATAGTGGCTGGGGGACTTGGGAATCAACCCACTGTCCTGGAGACGGCGGAAGCATTCCAC
NM_001271864.1      ATAGTGGCTGGGGGACTTGGGAATCAACCCACTGTCCTGGAGACGGCGGAAGCATTCCAC
NM_001271865.1      ATAGTGGCTGGGGGACTTGGGAATCAACCCACTGTCCTGGAGACGGCGGAAGCATTCCAC
                    ************************************************************

HsCD00287767        CCAGGGAAGAACAAATGGGAGATCCTCCCTGCCATGCCCACACCCCGCTGTGCCTGCTCC
NM_018203.2         CCAGGGAAGAACAAATGGGAGATCCTCCCTGCCATGCCCACACCCCGCTGTGCCTGCTCC
NM_001271863.1      CCAGGGAAGAACAAATGGGAGATCCTCCCTGCCATGCCCACACCCCGCTGTGCCTGCTCC
NM_001271864.1      CCAGGGAAGAACAAATGGGAGATCCTCCCTGCCATGCCCACACCCCGCTGTGCCTGCTCC
NM_001271865.1      CCAGGGAAGAACAAATGGGAGATCCTCCCTGCCATGCCCACACCCCGCTGTGCCTGCTCC
                    ************************************************************

HsCD00287767        AGCATAGTCGTCAAGAACTGCCTCCTCGCCGTGGGAGGTGTCAACCAGGGTCTGAGTGAC
NM_018203.2         AGCATAGTCGTCAAGAACTGCCTCCTCGCCGTGGGAGGTGTCAACCAGGGTCTGAGTGAC
NM_001271863.1      AGCATAGTCGTCAAGAACTGCCTCCTCGCCGTGGGAGGTGTCAACCAGGGTCTGAGTGAC
NM_001271864.1      AGCATAGTCGTCAAGAACTGCCTCCTCGCCGTGGGAGGTGTCAACCAGGGTCTGAGTGAC
NM_001271865.1      AGCATAGTCGTCAAGAACTGCCTCCTCGCCGTGGGAGGTGTCAACCAGGGTCTGAGTGAC
                    ************************************************************

HsCD00287767        GCAGTGGAGGCCCTGTGTGTCTCTGACTCCTAC
NM_018203.2         GCAGTGGAGGCCCTGTGTGTCTCTGACTCCTAG
NM_001271863.1      GCAGTGGAGGCCCTGTGTGTCTCTGACTCCTAG
NM_001271864.1      GCAGTGGAGGCCCTGTGTGTCTCTGACTCCTAG
NM_001271865.1      GCAGTGGAGGCCCTGTGTGTCTCTGACTCCTAG
                    ******************************** 

Protein

SMART domain : Kelch :
UniProt : Q8IYD2
HPRD : 07696

Clone

Cloning Information : 471004
HIP Master Clone ID : 393922
Original Clone ID : 12141

Organism


TAX_ID : 9606
Species Specific ID: 55220

Genome

Chromosome : 1
Map Location : 1q32.1
Ensembl : ENSG00000162873

Reagents

Labome : KLHDC8A-antibody