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ACTR6 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTR6
Gene Name:  ARP6 actin-related protein 6 homolog (yeast)
Sequence             
Original Clone ID: 33589
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00287816

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1191nts        
Open reading frame : 1 to 1191
Reference Sequence Alignment

ATGACGACCTTAGTGCTGGATAATGGAGCTTACAACGCCAAAATCGGTTACAGCCATGAA
AATGTGTCGGTTATTCCTAATTGTCAGTTCCGGTCAAAAACAGCACGTCTTAAAACTTTT
ACTGCCAACCAGATAGATGAAATAAAAGACCCTTCTGGACTCTTTTACATCCTCCCTTTT
CAAAAGGGCTACTTGGTGAATTGGGATGTTCAGAGACAAGTTTGGGATTACCTTTTTGGA
AAAGAAATGTATCAGGTTGATTTTTTAGATACTAATATTATTATCACTGAACCATACTTT
AACTTCACTTCAATTCAAGAATCAATGAATGAAATTCTATTTGAAGAATACCAGTTTCAA
GCAGTATTAAGAGTAAATGCTGGGGCTCTCAGTGCACATAGGTATTTCCGAGATAATCCT
TCCGAATTATGCTGTATCATTGTTGATAGTGGATATTCCTTTACACATATAGTTCCTTAT
TGTAGAAGTAAAAAGAAAAAAGAAGCAATTATTCGGATAAATGTGGGAGGAAAACTCTTA
ACCAATCATCTAAAGGAGATCATATCTTACAGGCAGCTACATGTTATGGATGAAACACAT
GTGATTAATCAAGTGAAAGAAGATGTATGCTATGTGTCTCAGGATTTTTATAGAGACATG
GATATTGCAAAGTTGAAAGGAGAAGAAAATACAGTAATGATAGACTATGTCTTGCCTGAC
TTCAGTACAATTAAAAAGGGCTTTTGTAAGCCAAGGGAAGAGATGGTGTTGAGTGGAAAA
TACAAATCTGGGGAACAAATTCTTCGTTTGGCCAATGAGAGATTTGCTGTTCCGGAAATA
CTCTTTAATCCTTCTGATATAGGCATTCAAGAAATGGGAATTCCAGAAGCTATTGTCTAT
TCAATTCAAAATCTACCTGAAGAAATGCAGCCGCATTTTTTTAAGAACATTGTCTTGACA
GGAGGAAATTCCCTTTTCCCAGGATTTAGGGATCGGGTTTACTCAGAAGTTCGATGTCTT
ACTCCAACAGATTATGATGTTTCTGTTGTGCTGCCTGAAAACCCTATTACTTATGCCTGG
GAAGGTGGAAAATTGATATCAGAGAATGATGATTTTGAAGATATGGTGGTAACAAGAGAA
GATTACGAAGAAAATGGACATAGCGTCTGTGAAGAGAAATTTGATATTTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1251
Start on reference sequence 61
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTR6
Symbol Nomenclature : ACTR6
SYNONYM : ARP6; HSPC281; MSTP136; hARP6; hARPX
Designation : actin-related protein 6
Full Nomenclature : ARP6 actin-related protein 6 homolog (yeast)
GENEID : 64431
Locus Tag : CDA12
HGNC : 24025
Vega : OTTHUMG00000170245
Target GenBank: BC015107.1

 

Reference Sequence Alignment

  

HsCD00287816      ATGACGACCTTAGTGCTGGATAATGGAGCTTACAACGCCAAAATCGGTTACAGCCATGAA
NM_022496.4       ATGACGACCTTAGTGCTGGATAATGGAGCTTACAACGCCAAAATCGGTTACAGCCATGAA
                  ************************************************************

HsCD00287816      AATGTGTCGGTTATTCCTAATTGTCAGTTCCGGTCAAAAACAGCACGTCTTAAAACTTTT
NM_022496.4       AATGTGTCGGTTATTCCTAATTGTCAGTTCCGGTCAAAAACAGCACGTCTTAAAACTTTT
                  ************************************************************

HsCD00287816      ACTGCCAACCAGATAGATGAAATAAAAGACCCTTCTGGACTCTTTTACATCCTCCCTTTT
NM_022496.4       ACTGCCAACCAGATAGATGAAATAAAAGACCCTTCTGGACTCTTTTACATCCTCCCTTTT
                  ************************************************************

HsCD00287816      CAAAAGGGCTACTTGGTGAATTGGGATGTTCAGAGACAAGTTTGGGATTACCTTTTTGGA
NM_022496.4       CAAAAGGGCTACTTGGTGAATTGGGATGTTCAGAGACAAGTTTGGGATTACCTTTTTGGA
                  ************************************************************

HsCD00287816      AAAGAAATGTATCAGGTTGATTTTTTAGATACTAATATTATTATCACTGAACCATACTTT
NM_022496.4       AAAGAAATGTATCAGGTTGATTTTTTAGATACTAATATTATTATCACTGAACCATACTTT
                  ************************************************************

HsCD00287816      AACTTCACTTCAATTCAAGAATCAATGAATGAAATTCTATTTGAAGAATACCAGTTTCAA
NM_022496.4       AACTTCACTTCAATTCAAGAATCAATGAATGAAATTCTATTTGAAGAATACCAGTTTCAA
                  ************************************************************

HsCD00287816      GCAGTATTAAGAGTAAATGCTGGGGCTCTCAGTGCACATAGGTATTTCCGAGATAATCCT
NM_022496.4       GCAGTATTAAGAGTAAATGCTGGGGCTCTCAGTGCACATAGGTATTTCCGAGATAATCCT
                  ************************************************************

HsCD00287816      TCCGAATTATGCTGTATCATTGTTGATAGTGGATATTCCTTTACACATATAGTTCCTTAT
NM_022496.4       TCCGAATTATGCTGTATCATTGTTGATAGTGGATATTCCTTTACACATATAGTTCCTTAT
                  ************************************************************

HsCD00287816      TGTAGAAGTAAAAAGAAAAAAGAAGCAATTATTCGGATAAATGTGGGAGGAAAACTCTTA
NM_022496.4       TGTAGAAGTAAAAAGAAAAAAGAAGCAATTATTCGGATAAATGTGGGAGGAAAACTCTTA
                  ************************************************************

HsCD00287816      ACCAATCATCTAAAGGAGATCATATCTTACAGGCAGCTACATGTTATGGATGAAACACAT
NM_022496.4       ACCAATCATCTAAAGGAGATCATATCTTACAGGCAGCTACATGTTATGGATGAAACACAT
                  ************************************************************

HsCD00287816      GTGATTAATCAAGTGAAAGAAGATGTATGCTATGTGTCTCAGGATTTTTATAGAGACATG
NM_022496.4       GTGATTAATCAAGTGAAAGAAGATGTATGCTATGTGTCTCAGGATTTTTATAGAGACATG
                  ************************************************************

HsCD00287816      GATATTGCAAAGTTGAAAGGAGAAGAAAATACAGTAATGATAGACTATGTCTTGCCTGAC
NM_022496.4       GATATTGCAAAGTTGAAAGGAGAAGAAAATACAGTAATGATAGACTATGTCTTGCCTGAC
                  ************************************************************

HsCD00287816      TTCAGTACAATTAAAAAGGGCTTTTGTAAGCCAAGGGAAGAGATGGTGTTGAGTGGAAAA
NM_022496.4       TTCAGTACAATTAAAAAGGGCTTTTGTAAGCCAAGGGAAGAGATGGTGTTGAGTGGAAAA
                  ************************************************************

HsCD00287816      TACAAATCTGGGGAACAAATTCTTCGTTTGGCCAATGAGAGATTTGCTGTTCCGGAAATA
NM_022496.4       TACAAATCTGGGGAACAAATTCTTCGTTTGGCCAATGAGAGATTTGCTGTTCCGGAAATA
                  ************************************************************

HsCD00287816      CTCTTTAATCCTTCTGATATAGGCATTCAAGAAATGGGAATTCCAGAAGCTATTGTCTAT
NM_022496.4       CTCTTTAATCCTTCTGATATAGGCATTCAAGAAATGGGAATTCCAGAAGCTATTGTCTAT
                  ************************************************************

HsCD00287816      TCAATTCAAAATCTACCTGAAGAAATGCAGCCGCATTTTTTTAAGAACATTGTCTTGACA
NM_022496.4       TCAATTCAAAATCTACCTGAAGAAATGCAGCCGCATTTTTTTAAGAACATTGTCTTGACA
                  ************************************************************

HsCD00287816      GGAGGAAATTCCCTTTTCCCAGGATTTAGGGATCGGGTTTACTCAGAAGTTCGATGTCTT
NM_022496.4       GGAGGAAATTCCCTTTTCCCAGGATTTAGGGATCGGGTTTACTCAGAAGTTCGATGTCTT
                  ************************************************************

HsCD00287816      ACTCCAACAGATTATGATGTTTCTGTTGTGCTGCCTGAAAACCCTATTACTTATGCCTGG
NM_022496.4       ACTCCAACAGATTATGATGTTTCTGTTGTGCTGCCTGAAAACCCTATTACTTATGCCTGG
                  ************************************************************

HsCD00287816      GAAGGTGGAAAATTGATATCAGAGAATGATGATTTTGAAGATATGGTGGTAACAAGAGAA
NM_022496.4       GAAGGTGGAAAATTGATATCAGAGAATGATGATTTTGAAGATATGGTGGTAACAAGAGAA
                  ************************************************************

HsCD00287816      GATTACGAAGAAAATGGACATAGCGTCTGTGAAGAGAAATTTGATATTTAC
NM_022496.4       GATTACGAAGAAAATGGACATAGCGTCTGTGAAGAGAAATTTGATATTTAA
                  **************************************************.

Protein

SMART domain : ACTIN : Actin
UniProt : Q9GZN1
HPRD : 12421

Clone

Cloning Information : 471053
HIP Master Clone ID : 393971
Original Clone ID : 33589

Organism


TAX_ID : 9606
Species Specific ID: 64431

Genome

Chromosome : 12
Map Location : 12q23.1
Ensembl : ENSG00000075089

Reagents

Labome : ACTR6-antibody