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LYSMD1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LYSMD1
Gene Name:  LysM, putative peptidoglycan-binding, domain containing 1
Sequence             
Original Clone ID: 44081
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00288004

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 684nts        
Open reading frame : 1 to 684
Reference Sequence Alignment

ATGGCTTCCCCGTCTAGACAGCCCCCGCCAGGGGGGTCAGGACTGCTTCAAGGGAGCCGG
GCTCGTTCATATGGAAGCCTGGTGCAATCGGCCTGCTCCCCAGTGAGGGAAAGACGCCTG
GAGCATCAGTTGGAGCCCGGAGACACCCTGGCTGGACTAGCACTCAAATATGGGGTGACG
ATGGAACAGATTAAACGTGCAAACCGCCTTTATACTAATGACTCCATCTTCCTGAAGAAA
ACCCTCTACATCCCCATCCTGACAGAGCCCAGAGACCTGTTCAATGGTTTGGACTCTGAG
GAAGAGAAAGATGGAGAGGAAAAAGTACACCCAAGTAACAGTGAAGTTTGGCCACACTCA
ACTGAGAGGAAGAAACAAGAGACAGGAGCAGGACGTGCCAATGGTGAAGTCCTCCCCACA
CCTGGCCAGGAAACCCCCACGCCCATCCATGACCTCTCTGCCTCTGATTTCCTTAAGAAG
CTTGATTCACAGATCAGCCTGTCCAAGAAGGCTGCTGCTCAGAAGCTGAAAAAAGGGGAA
AATGGGGTACCTGGGGAGGATGCAGGTCTCCACCTGAGCTCCCCTTGGATGCAGCAACGA
GCAGTCCTAGGTCCTGTGCCGCTGACCCGTACCTCTCGGACCCGGACACTACGGGACCAG
GAGGATGAAATCTTCAAACTCTGC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 241
End on reference sequence 924
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LYSMD1
Symbol Nomenclature : LYSMD1
SYNONYM : RP11-68I18.5
Designation : lysM and putative peptidoglycan-binding domain-containing protein 1
Full Nomenclature : LysM, putative peptidoglycan-binding, domain containing 1
GENEID : 388695
Locus Tag : SB145
HGNC : 32070
Vega : OTTHUMG00000012260
Target GenBank: BC031649.1

 

Reference Sequence Alignment

  

HsCD00288004      ATGGCTTCCCCGTCTAGACAGCCCCCGCCAGGGGGGTCAGGACTGCTTCAAGGGAGCCGG
NM_212551.4       ATGGCTTCCCCGTCTAGACAGCCCCCGCCAGGGGGGTCAGGACTGCTTCAAGGGAGCCGG
                  ************************************************************

HsCD00288004      GCTCGTTCATATGGAAGCCTGGTGCAATCGGCCTGCTCCCCAGTGAGGGAAAGACGCCTG
NM_212551.4       GCTCGTTCATATGGAAGCCTGGTGCAATCGGCCTGCTCCCCAGTGAGGGAAAGACGCCTG
                  ************************************************************

HsCD00288004      GAGCATCAGTTGGAGCCCGGAGACACCCTGGCTGGACTAGCACTCAAATATGGGGTGACG
NM_212551.4       GAGCATCAGTTGGAGCCCGGAGACACCCTGGCTGGACTAGCACTCAAATATGGGGTGACG
                  ************************************************************

HsCD00288004      ATGGAACAGATTAAACGTGCAAACCGCCTTTATACTAATGACTCCATCTTCCTGAAGAAA
NM_212551.4       ATGGAACAGATTAAACGTGCAAACCGCCTTTATACTAATGACTCCATCTTCCTGAAGAAA
                  ************************************************************

HsCD00288004      ACCCTCTACATCCCCATCCTGACAGAGCCCAGAGACCTGTTCAATGGTTTGGACTCTGAG
NM_212551.4       ACCCTCTACATCCCCATCCTGACAGAGCCCAGAGACCTGTTCAATGGTTTGGACTCTGAG
                  ************************************************************

HsCD00288004      GAAGAGAAAGATGGAGAGGAAAAAGTACACCCAAGTAACAGTGAAGTTTGGCCACACTCA
NM_212551.4       GAAGAGAAAGATGGAGAGGAAAAAGTACACCCAAGTAACAGTGAAGTTTGGCCACACTCA
                  ************************************************************

HsCD00288004      ACTGAGAGGAAGAAACAAGAGACAGGAGCAGGACGTGCCAATGGTGAAGTCCTCCCCACA
NM_212551.4       ACTGAGAGGAAGAAACAAGAGACAGGAGCAGGACGTGCCAATGGTGAAGTCCTCCCCACA
                  ************************************************************

HsCD00288004      CCTGGCCAGGAAACCCCCACGCCCATCCATGACCTCTCTGCCTCTGATTTCCTTAAGAAG
NM_212551.4       CCTGGCCAGGAAACCCCCACGCCCATCCATGACCTCTCTGCCTCTGATTTCCTTAAGAAG
                  ************************************************************

HsCD00288004      CTTGATTCACAGATCAGCCTGTCCAAGAAGGCTGCTGCTCAGAAGCTGAAAAAAGGGGAA
NM_212551.4       CTTGATTCACAGATCAGCCTGTCCAAGAAGGCTGCTGCTCAGAAGCTGAAAAAAGGGGAA
                  ************************************************************

HsCD00288004      AATGGGGTACCTGGGGAGGATGCAGGTCTCCACCTGAGCTCCCCTTGGATGCAGCAACGA
NM_212551.4       AATGGGGTACCTGGGGAGGATGCAGGTCTCCACCTGAGCTCCCCTTGGATGCAGCAACGA
                  ************************************************************

HsCD00288004      GCAGTCCTAGGTCCTGTGCCGCTGACCCGTACCTCTCGGACCCGGACACTACGGGACCAG
NM_212551.4       GCAGTCCTAGGTCCTGTGCCGCTGACCCGTACCTCTCGGACCCGGACACTACGGGACCAG
                  ************************************************************

HsCD00288004      GAGGATGAAATCTTCAAACTCTGC
NM_212551.4       GAGGATGAAATCTTCAAACTCTGA
                  ***********************.

Protein

SMART domain : LysM : Lysin motif
UniProt : Q96S90
HPRD : 18017

Clone

Cloning Information : 471241
HIP Master Clone ID : 394159
Original Clone ID : 44081

Organism


TAX_ID : 9606
Species Specific ID: 388695

Genome

Chromosome : 1
Map Location : 1q21.3
Ensembl : ENSG00000163155

Reagents

Labome : LYSMD1-antibody