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WSB1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  WSB1
Gene Name:  WD repeat and SOCS box containing 1
Sequence             
Original Clone ID: 40312
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00288292

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1266nts         Open reading frame : 1 to 1266
Reference Sequence Alignment

ATGGCCAGCTTTCCCCCGAGGGTCAACGAGAAAGAGATCGTGAGATTACGTACTATAGGT
GAACTTTTAGCTCCTGCAGCTCCTTTTGACAAGAAATGTGGTCGTGAAAATTGGACTGTT
GCTTTTGCTCCAGATGGTTCATACTTTGCTTGGTCACAAGGACATCGCACAGTAAAGCTT
GTTCCGTGGTCCCAGTGCCTTCAGAACTTTCTCTTGCATGGCACCAAGAATGTTACCAAT
TCAAGCAGTTTAAGATTGCCAAGACAAAATAGTGATGGTGGTCAGAAAAATAAGCCTCGT
GAACATATTATAGACTGTGGAGATATAGTCTGGAGTCTTGCTTTTGGGTCATCAGTTCCA
GAAAAACAGAGTCGCTGTGTAAATATAGAATGGCATCGCTTCAGATTTGGACAAGATCAG
CTACTTCTTGCTACAGGGTTGAACAATGGGCGTATCAAAATATGGGATGTATATACAGGA
AAACTCCTCCTTAACTTGGTAGATCATACTGAAGTGGTCAGAGATTTAACTTTTGCTCCA
GATGGAAGCTTGATCCTGGTGTCAGCTTCAAGAGACAAAACTCTCAGAGTATGGGACCTG
AAAGATGATGGAAACATGATGAAAGTATTGAGGGGGCATCAGAATTGGGTGTACAGCTGT
GCATTCTCTCCTGACTCTTCTATGCTGTGTTCAGTCGGAGCCAGTAAAGCAGTTTTCCTT
TGGAATATGGATAAATACACCATGATACGGAAACTAGAAGGACATCACCATGATGTGGTA
GCTTGTGACTTTTCTCCTGATGGAGCATTACTGGCTACTGCATCTTATGATACTCGAGTA
TATATCTGGGATCCACATAATGGAGACATTCTGATGGAATTTGGGCACCTGTTTCCCCCA
CCTACTCCAATATTTGCTGGAGGAGCAAATGACCGGTGGGTACGATCTGTATCTTTTAGC
CATGATGGACTGCATGTTGCAAGCCTTGCTGATGATAAAATGGTGAGGTTCTGGAGAATT
GATGAGGATTATCCAGTGCAAGTTGCACCTTTGAGCAATGGTCTTTGCTGTGCCTTCTCT
ACTGATGGCAGTGTTTTAGCTGCTGGGACACATGACGGAAGTGTGTATTTTTGGGCCACT
CCACGGCAGGTCCCTAGCCTGCAACATTTATGTCGCATGTCAATCCGAAGAGTGATGCCC
ACCCAAGAAGTTCAGGAGCTGCCGATTCCTTCCAAGCTTTTGGAGTTTCTCTCGTATCGT
ATTTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1529
Start on reference sequence 264
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGTTGGC

3' Linker Sequence:
5p (goi-stop)CCAACTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : WSB1
Symbol Nomenclature : WSB1
SYNONYM : SWIP1; WSB-1
Designation : SOCS box-containing WD protein SWiP-1|WD repeat and SOCS box-containing 1|WD repeat and SOCS box-containing protein 1
Full Nomenclature : WD repeat and SOCS box containing 1
GENEID : 26118
HGNC : 19221
MIM : 610091
Vega : OTTHUMG00000132293
Target GenBank: BC021110.1

 

Reference Sequence Alignment

  

HsCD00288292      ATGGCCAGCTTTCCCCCGAGGGTCAACGAGAAAGAGATCGTGAGATTACGTACTATAGGT
NM_015626.8       ATGGCCAGCTTTCCCCCGAGGGTCAACGAGAAAGAGATCGTGAGATTACGTACTATAGGT
                  ************************************************************

HsCD00288292      GAACTTTTAGCTCCTGCAGCTCCTTTTGACAAGAAATGTGGTCGTGAAAATTGGACTGTT
NM_015626.8       GAACTTTTAGCTCCTGCAGCTCCTTTTGACAAGAAATGTGGTCGTGAAAATTGGACTGTT
                  ************************************************************

HsCD00288292      GCTTTTGCTCCAGATGGTTCATACTTTGCTTGGTCACAAGGACATCGCACAGTAAAGCTT
NM_015626.8       GCTTTTGCTCCAGATGGTTCATACTTTGCTTGGTCACAAGGACATCGCACAGTAAAGCTT
                  ************************************************************

HsCD00288292      GTTCCGTGGTCCCAGTGCCTTCAGAACTTTCTCTTGCATGGCACCAAGAATGTTACCAAT
NM_015626.8       GTTCCGTGGTCCCAGTGCCTTCAGAACTTTCTCTTGCATGGCACCAAGAATGTTACCAAT
                  ************************************************************

HsCD00288292      TCAAGCAGTTTAAGATTGCCAAGACAAAATAGTGATGGTGGTCAGAAAAATAAGCCTCGT
NM_015626.8       TCAAGCAGTTTAAGATTGCCAAGACAAAATAGTGATGGTGGTCAGAAAAATAAGCCTCGT
                  ************************************************************

HsCD00288292      GAACATATTATAGACTGTGGAGATATAGTCTGGAGTCTTGCTTTTGGGTCATCAGTTCCA
NM_015626.8       GAACATATTATAGACTGTGGAGATATAGTCTGGAGTCTTGCTTTTGGGTCATCAGTTCCA
                  ************************************************************

HsCD00288292      GAAAAACAGAGTCGCTGTGTAAATATAGAATGGCATCGCTTCAGATTTGGACAAGATCAG
NM_015626.8       GAAAAACAGAGTCGCTGTGTAAATATAGAATGGCATCGCTTCAGATTTGGACAAGATCAG
                  ************************************************************

HsCD00288292      CTACTTCTTGCTACAGGGTTGAACAATGGGCGTATCAAAATATGGGATGTATATACAGGA
NM_015626.8       CTACTTCTTGCTACAGGGTTGAACAATGGGCGTATCAAAATATGGGATGTATATACAGGA
                  ************************************************************

HsCD00288292      AAACTCCTCCTTAACTTGGTAGATCATACTGAAGTGGTCAGAGATTTAACTTTTGCTCCA
NM_015626.8       AAACTCCTCCTTAACTTGGTAGATCATACTGAAGTGGTCAGAGATTTAACTTTTGCTCCA
                  ************************************************************

HsCD00288292      GATGGAAGCTTGATCCTGGTGTCAGCTTCAAGAGACAAAACTCTCAGAGTATGGGACCTG
NM_015626.8       GATGGAAGCTTGATCCTGGTGTCAGCTTCAAGAGACAAAACTCTCAGAGTATGGGACCTG
                  ************************************************************

HsCD00288292      AAAGATGATGGAAACATGATGAAAGTATTGAGGGGGCATCAGAATTGGGTGTACAGCTGT
NM_015626.8       AAAGATGATGGAAACATGATGAAAGTATTGAGGGGGCATCAGAATTGGGTGTACAGCTGT
                  ************************************************************

HsCD00288292      GCATTCTCTCCTGACTCTTCTATGCTGTGTTCAGTCGGAGCCAGTAAAGCAGTTTTCCTT
NM_015626.8       GCATTCTCTCCTGACTCTTCTATGCTGTGTTCAGTCGGAGCCAGTAAAGCAGTTTTCCTT
                  ************************************************************

HsCD00288292      TGGAATATGGATAAATACACCATGATACGGAAACTAGAAGGACATCACCATGATGTGGTA
NM_015626.8       TGGAATATGGATAAATACACCATGATACGGAAACTAGAAGGACATCACCATGATGTGGTA
                  ************************************************************

HsCD00288292      GCTTGTGACTTTTCTCCTGATGGAGCATTACTGGCTACTGCATCTTATGATACTCGAGTA
NM_015626.8       GCTTGTGACTTTTCTCCTGATGGAGCATTACTGGCTACTGCATCTTATGATACTCGAGTA
                  ************************************************************

HsCD00288292      TATATCTGGGATCCACATAATGGAGACATTCTGATGGAATTTGGGCACCTGTTTCCCCCA
NM_015626.8       TATATCTGGGATCCACATAATGGAGACATTCTGATGGAATTTGGGCACCTGTTTCCCCCA
                  ************************************************************

HsCD00288292      CCTACTCCAATATTTGCTGGAGGAGCAAATGACCGGTGGGTACGATCTGTATCTTTTAGC
NM_015626.8       CCTACTCCAATATTTGCTGGAGGAGCAAATGACCGGTGGGTACGATCTGTATCTTTTAGC
                  ************************************************************

HsCD00288292      CATGATGGACTGCATGTTGCAAGCCTTGCTGATGATAAAATGGTGAGGTTCTGGAGAATT
NM_015626.8       CATGATGGACTGCATGTTGCAAGCCTTGCTGATGATAAAATGGTGAGGTTCTGGAGAATT
                  ************************************************************

HsCD00288292      GATGAGGATTATCCAGTGCAAGTTGCACCTTTGAGCAATGGTCTTTGCTGTGCCTTCTCT
NM_015626.8       GATGAGGATTATCCAGTGCAAGTTGCACCTTTGAGCAATGGTCTTTGCTGTGCCTTCTCT
                  ************************************************************

HsCD00288292      ACTGATGGCAGTGTTTTAGCTGCTGGGACACATGACGGAAGTGTGTATTTTTGGGCCACT
NM_015626.8       ACTGATGGCAGTGTTTTAGCTGCTGGGACACATGACGGAAGTGTGTATTTTTGGGCCACT
                  ************************************************************

HsCD00288292      CCACGGCAGGTCCCTAGCCTGCAACATTTATGTCGCATGTCAATCCGAAGAGTGATGCCC
NM_015626.8       CCACGGCAGGTCCCTAGCCTGCAACATTTATGTCGCATGTCAATCCGAAGAGTGATGCCC
                  ************************************************************

HsCD00288292      ACCCAAGAAGTTCAGGAGCTGCCGATTCCTTCCAAGCTTTTGGAGTTTCTCTCGTATCGT
NM_015626.8       ACCCAAGAAGTTCAGGAGCTGCCGATTCCTTCCAAGCTTTTGGAGTTTCTCTCGTATCGT
                  ************************************************************

HsCD00288292      ATTTAC
NM_015626.8       ATTTAG
                  ***** 

Pathways

Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Protein

SMART domain : SOCS_box :
SMART domain : WD40 : WD40 repeats
SMART domain : SOCS : suppressors of cytokine signalling
UniProt : Q9Y6I7
HPRD : 15680

Clone

Cloning Information : 471529
HIP Master Clone ID : 394447
Original Clone ID : 40312

Organism


TAX_ID : 9606
Species Specific ID: 26118

Genome

Chromosome : 17
Map Location : 17q11.1
Ensembl : ENSG00000109046

Reagents

Labome : WSB1-antibody