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MGC3207 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  MRI1
Gene Name:  methylthioribose-1-phosphate isomerase 1
Sequence             
Original Clone ID: 2981
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00288781

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1110nts         Open reading frame : 1 to 1110
Reference Sequence Alignment

ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTG
CTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATC
CGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCC
GTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACTCGCCGCGCTCGTGGCCTTCGTGCGC
GACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTGTCAACATGGCCCGCGCCGCC
CGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTACGGAAGAGGCG
GTCCGGGAGAGAGTGATCTGCTGCACCGAGGACATGCTGGAGAAAGACCTCAGAGACAAC
CGAAGCATTGGGGACCTAGGAGCCCGCCACCTCCTGGAGCGGGTGGCCCCCAGCGGTGGC
AAGGTGACTGTGCTGACCCACTGTAACACTGGTGCTCTGGCCACCGCTGGCTATGGTACA
GCCCTAGGTGTGATTCGCTCACTGCACAGCCTGGGCCGCCTGGAGCATGCCTTCTGCACA
GAGACCCGGCCCTACAACCAGGGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGAGCAG
ATCCCCGCCACCCTTATCACCGACAGCATGGTGGCTGCTGCCATGGCCCATAGGGGCGTG
TCAGCTGTGGTCGTGGGAGCTGACCGCGTGGTTGCCAACGGCGACACAGCCAACAAGGTG
GGCACCTACCAGCTGGCCATTGTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCC
CCCAGCTCTTCATGTGACCTCCGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGA
CCGGGCCAGGAGCTGACCGATGTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTT
TGGAATCCTGCCTTCGATGTCACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAA
CTGGGGGTCTTTGCCCCTGAGGAGCTCCGGACAGCCCTAACCACCACCATCTCTTCCAGG
GATGGAACCCTAGATGGACCCCAGATGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1150
Start on reference sequence 41
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGTTGGC

3' Linker Sequence:
5p (goi-stop)CCAACTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MRI1
Symbol Nomenclature : MRI1
SYNONYM : MRDI; MTNA; Ypr118w
Designation : M1Pi|MTR-1-P isomerase|S-methyl-5-thioribose-1-phosphate isomerase 1|mediator of RhoA-dependent invasion|methylthioribose-1-phosphate isomerase|methylthioribose-1-phosphate isomerase homolog|translation initiation factor eIF-2B subunit alpha/beta/delta-like protein
Full Nomenclature : methylthioribose-1-phosphate isomerase 1
GENEID : 84245
Locus Tag : UNQ6390/PRO21135
HGNC : 28469
MIM : 615105
Vega : OTTHUMG00000180867
Target GenBank: BC001703.1

 

Reference Sequence Alignment

  

HsCD00288781        ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTG
NM_001031727.2      ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTG
                    ************************************************************

HsCD00288781        CTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATC
NM_001031727.2      CTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATC
                    ************************************************************

HsCD00288781        CGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCC
NM_001031727.2      CGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCC
                    ************************************************************

HsCD00288781        GTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACTCGCCGCGCTCGTGGCCTTCGTGCGC
NM_001031727.2      GTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACTCGCCGCGCTCGTGGCCTTCGTGCGC
                    ************************************************************

HsCD00288781        GACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTGTCAACATGGCCCGCGCCGCC
NM_001031727.2      GACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTGTCAACATGGCCCGCGCCGCC
                    ************************************************************

HsCD00288781        CGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTACGGAAGAGGCG
NM_001031727.2      CGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTACGGAAGAGGCG
                    ************************************************************

HsCD00288781        GTCCGGGAGAGAGTGATCTGCTGCACCGAGGACATGCTGGAGAAAGACCTCAGAGACAAC
NM_001031727.2      GTCCGGGAGAGAGTGATCTGCTGCACCGAGGACATGCTGGAGAAAGACCTCAGAGACAAC
                    ************************************************************

HsCD00288781        CGAAGCATTGGGGACCTAGGAGCCCGCCACCTCCTGGAGCGGGTGGCCCCCAGCGGTGGC
NM_001031727.2      CGAAGCATTGGGGACCTAGGAGCCCGCCACCTCCTGGAGCGGGTGGCCCCCAGCGGTGGC
                    ************************************************************

HsCD00288781        AAGGTGACTGTGCTGACCCACTGTAACACTGGTGCTCTGGCCACCGCTGGCTATGGTACA
NM_001031727.2      AAGGTGACTGTGCTGACCCACTGTAACACTGGTGCTCTGGCCACCGCTGGCTATGGTACA
                    ************************************************************

HsCD00288781        GCCCTAGGTGTGATTCGCTCACTGCACAGCCTGGGCCGCCTGGAGCATGCCTTCTGCACA
NM_001031727.2      GCCCTAGGTGTGATTCGCTCACTGCACAGCCTGGGCCGCCTGGAGCATGCCTTCTGCACA
                    ************************************************************

HsCD00288781        GAGACCCGGCCCTACAACCAGGGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGAGCAG
NM_001031727.2      GAGACCCGGCCCTACAACCAGGGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGAGCAG
                    ************************************************************

HsCD00288781        ATCCCCGCCACCCTTATCACCGACAGCATGGTGGCTGCTGCCATGGCCCATAGGGGCGTG
NM_001031727.2      ATCCCCGCCACCCTTATCACCGACAGCATGGTGGCTGCTGCCATGGCCCATAGGGGCGTG
                    ************************************************************

HsCD00288781        TCAGCTGTGGTCGTGGGAGCTGACCGCGTGGTTGCCAACGGCGACACAGCCAACAAGGTG
NM_001031727.2      TCAGCTGTGGTCGTGGGAGCTGACCGCGTGGTTGCCAACGGCGACACAGCCAACAAGGTG
                    ************************************************************

HsCD00288781        GGCACCTACCAGCTGGCCATTGTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCC
NM_001031727.2      GGCACCTACCAGCTGGCCATTGTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCC
                    ************************************************************

HsCD00288781        CCCAGCTCTTCATGTGACCTCCGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGA
NM_001031727.2      CCCAGCTCTTCATGTGACCTCCGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGA
                    ************************************************************

HsCD00288781        CCGGGCCAGGAGCTGACCGATGTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTT
NM_001031727.2      CCGGGCCAGGAGCTGACCGATGTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTT
                    ************************************************************

HsCD00288781        TGGAATCCTGCCTTCGATGTCACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAA
NM_001031727.2      TGGAATCCTGCCTTCGATGTCACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAA
                    ************************************************************

HsCD00288781        CTGGGGGTCTTTGCCCCTGAGGAGCTCCGGACAGCCCTAACCACCACCATCTCTTCCAGG
NM_001031727.2      CTGGGGGTCTTTGCCCCTGAGGAGCTCCGGACAGCCCTAACCACCACCATCTCTTCCAGG
                    ************************************************************

HsCD00288781        GATGGAACCCTAGATGGACCCCAGATGTAC
NM_001031727.2      GATGGAACCCTAGATGGACCCCAGATGTAA
                    *****************************.

Pathways

Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
Reactome : Metabolism of polyamines
Reactome : Methionine salvage pathway
Reactome : Sulfur amino acid metabolism

Protein

UniProt : Q9BV20
HPRD : 14548

Clone

Cloning Information : 472018
HIP Master Clone ID : 394936
Original Clone ID : 2981

Organism


TAX_ID : 9606
Species Specific ID: 84245

Genome

Chromosome : 19
Map Location : 19p13.2
Ensembl : ENSG00000037757

Reagents

Labome : MRI1-antibody