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TIMD4 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TIMD4
Gene Name:  T-cell immunoglobulin and mucin domain containing 4
Sequence             
Original Clone ID: 3478
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 24478428
Title: The role of phosphatidylserine receptors in enveloped virus infection
Authors: The ORFeome Collaboration
HsCD00288806

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1137nts         Open reading frame : 1 to 1137
Reference Sequence Alignment

ATGTCCAAAGAACCTCTCATTCTCTGGCTGATGATTGAGTTTTGGTGGCTTTACCTGACA
CCAGTCACTTCAGAGACTGTTGTGACGGAGGTTTTGGGTCACCGGGTGACTTTGCCCTGT
CTGTACTCATCCTGGTCTCACAACAGCAACAGCATGTGCTGGGGGAAAGACCAGTGCCCC
TACTCCGGTTGCAAGGAGGCGCTCATCCGCACTGATGGAATGAGGGTGACCTCAAGAAAG
TCAGCAAAATATAGACTTCAGGGGACTATCCCGAGAGGTGATGTCTCCTTGACCATCTTA
AACCCCAGTGAAAGTGACAGCGGTGTGTACTGCTGCCGCATAGAAGTGCCTGGCTGGTTC
AACGATGTAAAGATAAACGTGCGCCTGAATCTACAGAGAGCCTCAACAACCACGCACAGA
ACAGCAACCACCACCACACGCAGAACAACAACAACAAGCCCCACCACCACCCGACAAATG
ACAACAACCCCAGCTGCACTTCCAACAACAGTCGTGACCACACCCGATCTCACAACCGGA
ACACCACTCCAGATGACAACCATTGCCGTCTTCACAACAGCAAACACGTGCCTTTCACTA
ACCCCAAGCACCCTTCCGGAGGAAGCCACAGGTCTTCTGACTCCCGAGCCTTCTAAGGAA
GGGCCCATCCTCACTGCAGAATCAGAAACTGTCCTCCCCAGTGATTCCTGGAGTAGTGCT
GAGTCTACTTCTGCTGACACTGTCCTGCTGACATCCAAAGAGTCCAAAGTTTGGGATCTC
CCATCAACATCCCACGTGTCAATGTGGAAAACGAGTGATTCTGTGTCTTCTCCTCAGCCT
GGAGCATCTGATACAGCAGTTCCTGAGCAGAACAAAACAACAAAAACAGGACAGATGGAT
GGAATACCCATGTCAATGAAGAATGAAATGCCCATCTCCCAACTACTGATGATCATCGCC
CCCTCCTTGGGATTTGTGCTCTTCGCATTGTTTGTGGCGTTTCTCCTGAGAGGGAAACTC
ATGGAAACCTATTGTTCGCAGAAACACACAAGGCTAGACTACATTGGAGATAGTAAAAAT
GTCCTCAATGACGTGCAGCATGGAAGGGAAGACGAAGACGGCCTTTTTACCCTCTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1194
Start on reference sequence 58
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGTTGGC

3' Linker Sequence:
5p (goi-stop)CCAACTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TIMD4
Symbol Nomenclature : TIMD4
SYNONYM : SMUCKLER; TIM4
Designation : T-cell immunoglobulin and mucin domain-containing protein 4|T-cell immunoglobulin mucin receptor 4|T-cell membrane protein 4|TIM-4|TIMD-4
Full Nomenclature : T-cell immunoglobulin and mucin domain containing 4
GENEID : 91937
HGNC : 25132
MIM : 610096
Vega : OTTHUMG00000130244
Target GenBank: BC008988.1

 

Reference Sequence Alignment

  

HsCD00288806        ATGTCCAAAGAACCTCTCATTCTCTGGCTGATGATTGAGTTTTGGTGGCTTTACCTGACA
NM_138379.2         ATGTCCAAAGAACCTCTCATTCTCTGGCTGATGATTGAGTTTTGGTGGCTTTACCTGACA
NM_001146726.1      ATGTCCAAAGAACCTCTCATTCTCTGGCTGATGATTGAGTTTTGGTGGCTTTACCTGACA
                    ************************************************************

HsCD00288806        CCAGTCACTTCAGAGACTGTTGTGACGGAGGTTTTGGGTCACCGGGTGACTTTGCCCTGT
NM_138379.2         CCAGTCACTTCAGAGACTGTTGTGACGGAGGTTTTGGGTCACCGGGTGACTTTGCCCTGT
NM_001146726.1      CCAGTCACTTCAGAGACTGTTGTGACGGAGGTTTTGGGTCACCGGGTGACTTTGCCCTGT
                    ************************************************************

HsCD00288806        CTGTACTCATCCTGGTCTCACAACAGCAACAGCATGTGCTGGGGGAAAGACCAGTGCCCC
NM_138379.2         CTGTACTCATCCTGGTCTCACAACAGCAACAGCATGTGCTGGGGGAAAGACCAGTGCCCC
NM_001146726.1      CTGTACTCATCCTGGTCTCACAACAGCAACAGCATGTGCTGGGGGAAAGACCAGTGCCCC
                    ************************************************************

HsCD00288806        TACTCCGGTTGCAAGGAGGCGCTCATCCGCACTGATGGAATGAGGGTGACCTCAAGAAAG
NM_138379.2         TACTCCGGTTGCAAGGAGGCGCTCATCCGCACTGATGGAATGAGGGTGACCTCAAGAAAG
NM_001146726.1      TACTCCGGTTGCAAGGAGGCGCTCATCCGCACTGATGGAATGAGGGTGACCTCAAGAAAG
                    ************************************************************

HsCD00288806        TCAGCAAAATATAGACTTCAGGGGACTATCCCGAGAGGTGATGTCTCCTTGACCATCTTA
NM_138379.2         TCAGCAAAATATAGACTTCAGGGGACTATCCCGAGAGGTGATGTCTCCTTGACCATCTTA
NM_001146726.1      TCAGCAAAATATAGACTTCAGGGGACTATCCCGAGAGGTGATGTCTCCTTGACCATCTTA
                    ************************************************************

HsCD00288806        AACCCCAGTGAAAGTGACAGCGGTGTGTACTGCTGCCGCATAGAAGTGCCTGGCTGGTTC
NM_138379.2         AACCCCAGTGAAAGTGACAGCGGTGTGTACTGCTGCCGCATAGAAGTGCCTGGCTGGTTC
NM_001146726.1      AACCCCAGTGAAAGTGACAGCGGTGTGTACTGCTGCCGCATAGAAGTGCCTGGCTGGTTC
                    ************************************************************

HsCD00288806        AACGATGTAAAGATAAACGTGCGCCTGAATCTACAGAGAGCCTCAACAACCACGCACAGA
NM_138379.2         AACGATGTAAAGATAAACGTGCGCCTGAATCTACAGAGAGCCTCAACAACCACGCACAGA
NM_001146726.1      AACGATGTAAAGATAAACGTGCGCCTGAATCTACAGAGAGCCTCAACAACCACGCACAGA
                    ************************************************************

HsCD00288806        ACAGCAACCACCACCACACGCAGAACAACAACAACAAGCCCCACCACCACCCGACAAATG
NM_138379.2         ACAGCAACCACCACCACACGCAGAACAACAACAACAAGCCCCACCACCACCCGACAAATG
NM_001146726.1      ACAGCAACCACCACCACACGCAGAACAACAACAACAAGCCCCACCACCACCCGACAAATG
                    ************************************************************

HsCD00288806        ACAACAACCCCAGCTGCACTTCCAACAACAGTCGTGACCACACCCGATCTCACAACCGGA
NM_138379.2         ACAACAACCCCAGCTGCACTTCCAACAACAGTCGTGACCACACCCGATCTCACAACCGGA
NM_001146726.1      ACAACAACCCCAGCTGCACTTCCAACAACAGTCGTGACCACACCCGATCTCACAACCGGA
                    ************************************************************

HsCD00288806        ACACCACTCCAGATGACAACCATTGCCGTCTTCACAACAGCAAACACGTGCCTTTCACTA
NM_138379.2         ACACCACTCCAGATGACAACCATTGCCGTCTTCACAACAGCAAACACGTGCCTTTCACTA
NM_001146726.1      ACACCACTCCAGATGACAACCATTGCCGTCTTCACAACAGCAAACACGTGCCTTTCACTA
                    ************************************************************

HsCD00288806        ACCCCAAGCACCCTTCCGGAGGAAGCCACAGGTCTTCTGACTCCCGAGCCTTCTAAGGAA
NM_138379.2         ACCCCAAGCACCCTTCCGGAGGAAGCCACAGGTCTTCTGACTCCCGAGCCTTCTAAGGAA
NM_001146726.1      ACCCCAAGCACCCTTCCGGAGGAAGCCACAGGTCTTCTGACTCCCGAGCCTTCTAAGGAA
                    ************************************************************

HsCD00288806        GGGCCCATCCTCACTGCAGAATCAGAAACTGTCCTCCCCAGTGATTCCTGGAGTAGTGCT
NM_138379.2         GGGCCCATCCTCACTGCAGAATCAGAAACTGTCCTCCCCAGTGATTCCTGGAGTAGTGTT
NM_001146726.1      GGGCCCATCCTCACTGCAGAATCAGAAACTGTCCTCCCCAGTGATTCCTGGAGTAGTGTT
                    ********************************************************** *

HsCD00288806        GAGTCTACTTCTGCTGACACTGTCCTGCTGACATCCAAAGAGTCCAAAGTTTGGGATCTC
NM_138379.2         GAGTCTACTTCTGCTGACACTGTCCTGCTGACATCCAAAGAGTCCAAAGTTTGGGATCTC
NM_001146726.1      GAGTCTACTTCTGCTGACACTGTCCTGCTGACATCCAAAG--------------------
                    ****************************************                    

HsCD00288806        CCATCAACATCCCACGTGTCAATGTGGAAAACGAGTGATTCTGTGTCTTCTCCTCAGCCT
NM_138379.2         CCATCAACATCCCACGTGTCAATGTGGAAAACGAGTGATTCTGTGTCTTCTCCTCAGCCT
NM_001146726.1      ------------------------------------------------------------
                                                                                

HsCD00288806        GGAGCATCTGATACAGCAGTTCCTGAGCAGAACAAAACAACAAAAACAGGACAGATGGAT
NM_138379.2         GGAGCATCTGATACAGCAGTTCCTGAGCAGAACAAAACAACAAAAACAGGACAGATGGAT
NM_001146726.1      ----CATCTGATACAGCAGTTCCTGAGCAGAACAAAACAACAAAAACAGGACAGATGGAT
                        ********************************************************

HsCD00288806        GGAATACCCATGTCAATGAAGAATGAAATGCCCATCTCCCAACTACTGATGATCATCGCC
NM_138379.2         GGAATACCCATGTCAATGAAGAATGAAATGCCCATCTCCCAACTACTGATGATCATCGCC
NM_001146726.1      GGAATACCCATGTCAATGAAGAATGAAATGCCCATCTCCCAACTACTGATGATCATCGCC
                    ************************************************************

HsCD00288806        CCCTCCTTGGGATTTGTGCTCTTCGCATTGTTTGTGGCGTTTCTCCTGAGAGGGAAACTC
NM_138379.2         CCCTCCTTGGGATTTGTGCTCTTCGCATTGTTTGTGGCGTTTCTCCTGAGAGGGAAACTC
NM_001146726.1      CCCTCCTTGGGATTTGTGCTCTTCGCATTGTTTGTGGCGTTTCTCCTGAGAGGGAAACTC
                    ************************************************************

HsCD00288806        ATGGAAACCTATTGTTCGCAGAAACACACAAGGCTAGACTACATTGGAGATAGTAAAAAT
NM_138379.2         ATGGAAACCTATTGTTCGCAGAAACACACAAGGCTAGACTACATTGGAGATAGTAAAAAT
NM_001146726.1      ATGGAAACCTATTGTTCGCAGAAACACACAAGGCTAGACTACATTGGAGATAGTAAAAAT
                    ************************************************************

HsCD00288806        GTCCTCAATGACGTGCAGCATGGAAGGGAAGACGAAGACGGCCTTTTTACCCTCTAC
NM_138379.2         GTCCTCAATGACGTGCAGCATGGAAGGGAAGACGAAGACGGCCTTTTTACCCTCTAA
NM_001146726.1      GTCCTCAATGACGTGCAGCATGGAAGGGAAGACGAAGACGGCCTTTTTACCCTCTAA
                    ********************************************************.

Protein

SMART domain : IG : Immunoglobulin
UniProt : B5MCL9
UniProt : Q96H15
HPRD : 15508

Clone

Cloning Information : 472043
HIP Master Clone ID : 394961
Original Clone ID : 3478

Organism


TAX_ID : 9606
Species Specific ID: 91937

Genome

Chromosome : 5
Map Location : 5q33.3
Ensembl : ENSG00000145850

Reagents

Labome : TIMD4-antibody