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AQP11 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  AQP11
Gene Name:  aquaporin 11
Sequence             
Original Clone ID: 35922
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00289094

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 816nts         Open reading frame : 1 to 816
Reference Sequence Alignment

ATGTCGCCGCTGCTGGGGCTCCGGTCCGAGCTGCAGGACACCTGCACCTCGCTGGGACTG
ATGCTGTCGGTGGTGCTGCTCATGGGGCTGGCCCGCGTAGTCGCCCGGCAGCAGCTGCAC
AGGCCGGTGGCCCACGCCTTCGTCCTGGAGTTTCTAGCCACCTTCCAGCTCTGCTGCTGC
ACCCACGAGCTGCAACTGCTGAGCGAACAGCACCCCGCGCACCCCACCTGGACGCTGACG
CTCGTCTACTTCTTCTCGCTTGTGCATGGCCTGACTCTGGTGGGCACGTCCAGCAACCCG
TGCGGCGTGATGATGCAGATGATGCTGGGGGGCATGTCCCCCGAGACGGGTGCGGTGAGG
CTATTGGCTCAGCTGGTTAGTGCCCTGTGCAGCAGGTACTGCACAAGCGCCTTGTGGAGC
TTGGGTCTGACCCAGTATCACGTCAGCGAGAGGAGCTTCGCTTGCAAGAATCCCATCCGA
GTCGACTTGCTCAAAGCGGTCATCACAGAGGCCGTCTGCTCCTTTCTCTTCCACAGCGCT
CTGCTGCACTTCCAGGAAGTCCGAACCAAGCTTCGTATCCACCTGCTGGCTGCACTCATC
ACCTTTTTGGTCTATGCAGGAGGAAGTCTAACAGGAGCTGTATTTAATCCAGCTTTGGCA
CTTTCGCTACATTTCATGTGTTTTGATGAAGCATTCCCTCAGTTTTTTATAGTATACTGG
CTGGCTCCTTCTTTAGGTATATTGTTGATGATTTTGATGTTCAGCTTTTTCCTTCCATGG
CTGCATAACAACCATACAATTAATAAAAAGGAATAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1174
Start on reference sequence 359
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGTTGGC

3' Linker Sequence:
5p (goi-stop)CCAACTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : AQP11
Symbol Nomenclature : AQP11
SYNONYM : AQPX1
Designation : AQP-11|aquaporin-11
Full Nomenclature : aquaporin 11
GENEID : 282679
Locus Tag : PSEC0027
HGNC : 19940
MIM : 609914
Vega : OTTHUMG00000165194
Target GenBank: BC040443.1

 

Reference Sequence Alignment

  

HsCD00289094      ATGTCGCCGCTGCTGGGGCTCCGGTCCGAGCTGCAGGACACCTGCACCTCGCTGGGACTG
NM_173039.2       ATGTCGCCGCTGCTGGGGCTCCGGTCCGAGCTGCAGGACACCTGCACCTCGCTGGGACTG
                  ************************************************************

HsCD00289094      ATGCTGTCGGTGGTGCTGCTCATGGGGCTGGCCCGCGTAGTCGCCCGGCAGCAGCTGCAC
NM_173039.2       ATGCTGTCGGTGGTGCTGCTCATGGGGCTGGCCCGCGTAGTCGCCCGGCAGCAGCTGCAC
                  ************************************************************

HsCD00289094      AGGCCGGTGGCCCACGCCTTCGTCCTGGAGTTTCTAGCCACCTTCCAGCTCTGCTGCTGC
NM_173039.2       AGGCCGGTGGCCCACGCCTTCGTCCTGGAGTTTCTAGCCACCTTCCAGCTCTGCTGCTGC
                  ************************************************************

HsCD00289094      ACCCACGAGCTGCAACTGCTGAGCGAACAGCACCCCGCGCACCCCACCTGGACGCTGACG
NM_173039.2       ACCCACGAGCTGCAACTGCTGAGCGAACAGCACCCCGCGCACCCCACCTGGACGCTGACG
                  ************************************************************

HsCD00289094      CTCGTCTACTTCTTCTCGCTTGTGCATGGCCTGACTCTGGTGGGCACGTCCAGCAACCCG
NM_173039.2       CTCGTCTACTTCTTCTCGCTTGTGCATGGCCTGACTCTGGTGGGCACGTCCAGCAACCCG
                  ************************************************************

HsCD00289094      TGCGGCGTGATGATGCAGATGATGCTGGGGGGCATGTCCCCCGAGACGGGTGCGGTGAGG
NM_173039.2       TGCGGCGTGATGATGCAGATGATGCTGGGGGGCATGTCCCCCGAGACGGGTGCGGTGAGG
                  ************************************************************

HsCD00289094      CTATTGGCTCAGCTGGTTAGTGCCCTGTGCAGCAGGTACTGCACAAGCGCCTTGTGGAGC
NM_173039.2       CTATTGGCTCAGCTGGTTAGTGCCCTGTGCAGCAGGTACTGCACAAGCGCCTTGTGGAGC
                  ************************************************************

HsCD00289094      TTGGGTCTGACCCAGTATCACGTCAGCGAGAGGAGCTTCGCTTGCAAGAATCCCATCCGA
NM_173039.2       TTGGGTCTGACCCAGTATCACGTCAGCGAGAGGAGCTTCGCTTGCAAGAATCCCATCCGA
                  ************************************************************

HsCD00289094      GTCGACTTGCTCAAAGCGGTCATCACAGAGGCCGTCTGCTCCTTTCTCTTCCACAGCGCT
NM_173039.2       GTCGACTTGCTCAAAGCGGTCATCACAGAGGCCGTCTGCTCCTTTCTCTTCCACAGCGCT
                  ************************************************************

HsCD00289094      CTGCTGCACTTCCAGGAAGTCCGAACCAAGCTTCGTATCCACCTGCTGGCTGCACTCATC
NM_173039.2       CTGCTGCACTTCCAGGAAGTCCGAACCAAGCTTCGTATCCACCTGCTGGCTGCACTCATC
                  ************************************************************

HsCD00289094      ACCTTTTTGGTCTATGCAGGAGGAAGTCTAACAGGAGCTGTATTTAATCCAGCTTTGGCA
NM_173039.2       ACCTTTTTGGTCTATGCAGGAGGAAGTCTAACAGGAGCTGTATTTAATCCAGCTTTGGCA
                  ************************************************************

HsCD00289094      CTTTCGCTACATTTCATGTGTTTTGATGAAGCATTCCCTCAGTTTTTTATAGTATACTGG
NM_173039.2       CTTTCGCTACATTTCATGTGTTTTGATGAAGCATTCCCTCAGTTTTTTATAGTATACTGG
                  ************************************************************

HsCD00289094      CTGGCTCCTTCTTTAGGTATATTGTTGATGATTTTGATGTTCAGCTTTTTCCTTCCATGG
NM_173039.2       CTGGCTCCTTCTTTAGGTATATTGTTGATGATTTTGATGTTCAGCTTTTTCCTTCCATGG
                  ************************************************************

HsCD00289094      CTGCATAACAACCATACAATTAATAAAAAGGAATAC
NM_173039.2       CTGCATAACAACCATACAATTAATAAAAAGGAATAA
                  ***********************************.

Pathways

Reactome : Aquaporin-mediated transport
Reactome : Passive transport by Aquaporins
Reactome : Transmembrane transport of small molecules

Protein

UniProt : Q8NBQ7
HPRD : 16503

Clone

Cloning Information : 472331
HIP Master Clone ID : 395249
Original Clone ID : 35922

Organism


TAX_ID : 9606
Species Specific ID: 282679

Genome

Chromosome : 11
Map Location : 11q14.1
Ensembl : ENSG00000178301

Reagents

Labome : AQP11-antibody