DNASU Plasmid Repository • 480.965.5697 | Email

TUBG1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  TUBG1
Gene Name:  tubulin, gamma 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH027654.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00002895

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1356nts         Open reading frame : 1 to 1356
Reference Sequence Alignment

ATGCCGAGGGAAATCATCACCCTACAGTTGGGCCAGTGCGGCAATCAGATTGGGTTCGAG
TTCTGGAAACAGCTGTGCGCCGAGCATGGTATCAGCCCCGAGGGCATCGTGGAGGAGTTC
GCCACCGAGGGCACTGACCGCAAGGACGTCTTTTTCTACCAGGCAGACGATGAGCACTAC
ATCCCCCGGGCCGTGCTGCTGGACTTGGAACCCCGGGTGATCCACTCCATCCTCAACTCC
CCCTATGCCAAGCTCTACAACCCAGAGAACATCTACCTGTCGGAACATGGAGGAGGAGCT
GGCAACAACTGGGCCAGCGGATTCTCCCAGGGAGAAAAGATCCATGAGGACATTTTTGAC
ATCATAGACCGGGAGGCAGATGGTAGTGACAGTCTAGAGGGCTTTGTGCTGTGTCACTCC
ATTGCTGGGGGGACAGGCTCTGGACTGGGTTCCTACCTCTTAGAACGGCTGAATGACAGG
TATCCTAAGAAGCTGGTGCAGACATACTCAGTGTTTCCCAACCAGGACGAGATGAGCGAT
GTGGTGGTCCAGCCTTACAATTCACTCCTCACACTCAAGAGGCTGACGCAGAATGCAGAC
TGTGTGGTGGTGCTGGACAACACAGCCCTGAACCGGATTGCCACAGACCGCCTGCACATC
CAGAACCCATCCTTCTCCCAGATCAACCAGCTGGTGTCTACCATCATGTCAGCCAGCACC
ACCACCCTGCGCTACCCTGGCTACATGAACAATGACCTCATCGGCCTCATCGCCTCGCTC
ATTCCCACCCCACGGCTCCACTTCCTCATGACCGGCTACACCCCTCTCACTACGGACCAG
TCAGTGGCCAGCGTGAGGAAGACCACGGTCCTGGATGTCATGAGGCGGCTGCTGCAGCCC
AAGAACGTGATGGTGTCCACAGGCCGAGACCGCCAGACCAACCACTGCTACATCGCCATC
CTCAACATCATCCAGGGAGAGGTGGACCCCACCCAGGTCCACAAGAGCTTGCAGAGGATC
CGGGAACGCAAGTTGGCCAACTTCATCCCGTGGGGCCCCGCCAGCATCCAGGTGGCCCTG
TCGAGGAAGTCTCCCTACCTGCCCTCGGCCCACCGGGTCAGCGGGCTCATGATGGCCAAC
CACACCAGCATCTCCTCGCTCTTCGAGAGAACCTGTCGCCAGTATGACAAGCTGCGTAAG
CGGGAGGCCTTCCTGGAGCAGTTCCGCAAGGAGGACATGTTCAAGGACAACTTTGATGAG
ATGGACACATCCAGGGAGATTGTGCAGCAGCTCATCGATGAGTACCATGCGGCCACACGG
CCAGACTACATCTCCTGGGGCACCCAGGAGCAGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1427
Start on reference sequence 72
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TUBG1
Symbol Nomenclature : TUBG1
SYNONYM : CDCBM4; GCP-1; TUBG; TUBGCP1
Designation : gamma-tubulin complex component 1|tubulin gamma-1 chain|tubulin, gamma polypeptide
Full Nomenclature : tubulin, gamma 1
GENEID : 7283
GI : 61372098
GenBank Accession : AY891858
HGNC : 12417
MIM : 191135
Vega : OTTHUMG00000180277
Target GenBank: BC000619

 

Reference Sequence Alignment

  

HsCD00002895        ATGCCGAGGGAAATCATCACCCTACAGTTGGGCCAGTGCGGCAATCAGATTGGGTTCGAG
XM_005257222.1      ATGCCCCGGGAGATCATCACCCTGCAGCTGGGCCAGTGCGGCAACCAGATTGGGTTCGAG
NM_016437.2         ATGCCCCGGGAGATCATCACCCTGCAGCTGGGCCAGTGCGGCAACCAGATTGGGTTCGAG
NM_001070.4         ATGCCGAGGGAAATCATCACCCTACAGTTGGGCCAGTGCGGCAATCAGATTGGGTTCGAG
                    ***** .****.***********.*** **************** ***************

HsCD00002895        TTCTGGAAACAGCTGTGCGCCGAGCATGGTATCAGCCCCGAGGGCATCGTGGAGGAGTTC
XM_005257222.1      TTCTGGAAACAGCTGTGCGCCGAGCATGGTATCAGCCCCGAGGGCATCGTGGAGGAATTC
NM_016437.2         TTCTGGAAACAGCTGTGCGCCGAGCATGGTATCAGCCCCGAGGGCATCGTGGAGGAATTC
NM_001070.4         TTCTGGAAACAGCTGTGCGCCGAGCATGGTATCAGCCCCGAGGGCATCGTGGAGGAGTTC
                    ********************************************************.***

HsCD00002895        GCCACCGAGGGCACTGACCGCAAGGACGTCTTTTTCTACCAGGCAGACGATGAGCACTAC
XM_005257222.1      GCCACCGAGGGCACTGACCGCAAGGACGTCTTTTTCTACCAGGCAGACGATGAGCACTAC
NM_016437.2         GCCACCGAGGGCACTGACCGCAAGGACGTCTTTTTCTACCAGGCAGACGATGAGCACTAC
NM_001070.4         GCCACCGAGGGCACTGACCGCAAGGACGTCTTTTTCTACCAGGCAGACGATGAGCACTAC
                    ************************************************************

HsCD00002895        ATCCCCCGGGCCGTGCTGCTGGACTTGGAACCCCGGGTGATCCACTCCATCCTCAACTCC
XM_005257222.1      ATCCCCCGGGCCGTGCTGCTGGACTTGGAACCCCGGGTGATCCACTCCATCCTCAACTCC
NM_016437.2         ATCCCCCGGGCCGTGCTGCTGGACTTGGAACCCCGGGTGATCCACTCCATCCTCAACTCC
NM_001070.4         ATCCCCCGGGCCGTGCTGCTGGACTTGGAACCCCGGGTGATCCACTCCATCCTCAACTCC
                    ************************************************************

HsCD00002895        CCCTATGCCAAGCTCTACAACCCAGAGAACATCTACCTGTCGGAACATGGAGGAGGAGCT
XM_005257222.1      CCCTATGCCAAGCTCTACAACCCAGAGAACATCTACCTGTCGGAACATGGAGGAGGAGCT
NM_016437.2         CCCTATGCCAAGCTCTACAACCCAGAGAACATCTACCTGTCGGAACATGGAGGAGGAGCT
NM_001070.4         CCCTATGCCAAGCTCTACAACCCAGAGAACATCTACCTGTCGGAACATGGAGGAGGAGCT
                    ************************************************************

HsCD00002895        GGCAACAACTGGGCCAGCGGATTCTCCCAGGGAGAAAAGATCCATGAGGACATTTTTGAC
XM_005257222.1      GGCAACAACTGGGCCAGCGGATTCTCCCAGGGTGAGAAAATTCATGAAGACATCTTTGAC
NM_016437.2         GGCAACAACTGGGCCAGCGGATTCTCCCAGGGTGAGAAAATTCATGAAGACATCTTTGAC
NM_001070.4         GGCAACAACTGGGCCAGCGGATTCTCCCAGGGAGAAAAGATCCATGAGGACATTTTTGAC
                    ********************************:**.**.** *****.***** ******

HsCD00002895        ATCATAGACCGGGAGGCAGATGGTAGTGACAGTCTAG-----------------------
XM_005257222.1      ATCATAGACCGAGAAGCAGATGGAAGTGACAGTTTGGAGCTCCAGAACAGCTCCAGAGTG
NM_016437.2         ATCATAGACCGAGAAGCAGATGGAAGTGACAGTTTG------------------------
NM_001070.4         ATCATAGACCGGGAGGCAGATGGTAGTGACAGTCTAG-----------------------
                    ***********.**.********:********* *.                        

HsCD00002895        ----AGGGCTTTGTGCTGTGTCACTCCATTGCTGGGGGGACAGGCTCTGGACTGGGTTCC
XM_005257222.1      ATAAAGGGCTTCGTGCTGTGTCACTCCATCGCTGGGGGTACGGGTTCTGGCCTGGGCTCC
NM_016437.2         ---GAGGGCTTCGTGCTGTGTCACTCCATCGCTGGGGGTACGGGTTCTGGCCTGGGCTCC
NM_001070.4         ----AGGGCTTTGTGCTGTGTCACTCCATTGCTGGGGGGACAGGCTCTGGACTGGGTTCC
                        ******* ***************** ******** **.** *****.***** ***

HsCD00002895        TACCTCTTAGAACGGCTGAATGACAGGTATCCTAAGAAGCTGGTGCAGACATACTCAGTG
XM_005257222.1      TACCTCCTGGAGCGACTGAATGACAGGTACCCCAAGAAGCTAGTGCAGACTTATTCAGTG
NM_016437.2         TACCTCCTGGAGCGACTGAATGACAGGTACCCCAAGAAGCTAGTGCAGACTTATTCAGTG
NM_001070.4         TACCTCTTAGAACGGCTGAATGACAGGTATCCTAAGAAGCTGGTGCAGACATACTCAGTG
                    ****** *.**.**.************** ** ********.********:** ******

HsCD00002895        TTTCCCAACCAGGACGAGATGAGCGATGTGGTGGTCCAGCCTTACAATTCACTCCTCACA
XM_005257222.1      TTTCCCTACCAGGACGAGATGAGCGACGTAGTGGTTCAGCCCTACAATTCACTCCTGACA
NM_016437.2         TTTCCCTACCAGGACGAGATGAGCGACGTAGTGGTTCAGCCCTACAATTCACTCCTGACA
NM_001070.4         TTTCCCAACCAGGACGAGATGAGCGATGTGGTGGTCCAGCCTTACAATTCACTCCTCACA
                    ******:******************* **.***** ***** ************** ***

HsCD00002895        CTCAAGAGGCTGACGCAGAATGCAGACTGTGTGGTGGTGCTGGACAACACAGCCCTGAAC
XM_005257222.1      CTCAAGAGGCTGACGCAGAACGCAGATTGTGTGGTGGTGCTGGACAACACAGCCCTGAAC
NM_016437.2         CTCAAGAGGCTGACGCAGAACGCAGATTGTGTGGTGGTGCTGGACAACACAGCCCTGAAC
NM_001070.4         CTCAAGAGGCTGACGCAGAATGCAGACTGTGTGGTGGTGCTGGACAACACAGCCCTGAAC
                    ******************** ***** *********************************

HsCD00002895        CGGATTGCCACAGACCGCCTGCACATCCAGAACCCATCCTTCTCCCAGATCAACCAGCTG
XM_005257222.1      CGGATTGCCACAGACCGCCTGCACATCCAGAACCCGTCCTTCTCCCAGATCAACCAGCTG
NM_016437.2         CGGATTGCCACAGACCGCCTGCACATCCAGAACCCGTCCTTCTCCCAGATCAACCAGCTG
NM_001070.4         CGGATTGCCACAGACCGCCTGCACATCCAGAACCCATCCTTCTCCCAGATCAACCAGCTG
                    ***********************************.************************

HsCD00002895        GTGTCTACCATCATGTCAGCCAGCACCACCACCCTGCGCTACCCTGGCTACATGAACAAT
XM_005257222.1      GTGTCCACCATCATGTCGGCCAGCACCACCACCCTGCGCTACCCCGGCTACATGAACAAT
NM_016437.2         GTGTCCACCATCATGTCGGCCAGCACCACCACCCTGCGCTACCCCGGCTACATGAACAAT
NM_001070.4         GTGTCTACCATCATGTCAGCCAGCACCACCACCCTGCGCTACCCTGGCTACATGAACAAT
                    ***** ***********.************************** ***************

HsCD00002895        GACCTCATCGGCCTCATCGCCTCGCTCATTCCCACCCCACGGCTCCACTTCCTCATGACC
XM_005257222.1      GACCTCATCGGCCTCATCGCCTCGCTCATTCCCACCCCACGGCTCCACTTCCTCATGACC
NM_016437.2         GACCTCATCGGCCTCATCGCCTCGCTCATTCCCACCCCACGGCTCCACTTCCTCATGACC
NM_001070.4         GACCTCATCGGCCTCATCGCCTCGCTCATTCCCACCCCACGGCTCCACTTCCTCATGACC
                    ************************************************************

HsCD00002895        GGCTACACCCCTCTCACTACGGACCAGTCAGTGGCCAGCGTGAGGAAGACCACGGTCCTG
XM_005257222.1      GGCTACACCCCGCTCACTACAGACCAGTCAGTGGCCAGCGTGAGGAAGACCACGGTCCTG
NM_016437.2         GGCTACACCCCGCTCACTACAGACCAGTCAGTGGCCAGCGTGAGGAAGACCACGGTCCTG
NM_001070.4         GGCTACACCCCTCTCACTACGGACCAGTCAGTGGCCAGCGTGAGGAAGACCACGGTCCTG
                    *********** ********.***************************************

HsCD00002895        GATGTCATGAGGCGGCTGCTGCAGCCCAAGAACGTGATGGTGTCCACAGGCCGAGACCGC
XM_005257222.1      GATGTCATGAGGCGGCTGCTGCAGCCCAAGAACGTGATGGTGTCCACAGGCCGAGACCGC
NM_016437.2         GATGTCATGAGGCGGCTGCTGCAGCCCAAGAACGTGATGGTGTCCACAGGCCGAGACCGC
NM_001070.4         GATGTCATGAGGCGGCTGCTGCAGCCCAAGAACGTGATGGTGTCCACAGGCCGAGACCGC
                    ************************************************************

HsCD00002895        CAGACCAACCACTGCTACATCGCCATCCTCAACATCATCCAGGGAGAGGTGGACCCCACC
XM_005257222.1      CAGACCAACCACTGCTACATCGCCATCCTCAACATCATCCAGGGAGAGGTGGACCCCACC
NM_016437.2         CAGACCAACCACTGCTACATCGCCATCCTCAACATCATCCAGGGAGAGGTGGACCCCACC
NM_001070.4         CAGACCAACCACTGCTACATCGCCATCCTCAACATCATCCAGGGAGAGGTGGACCCCACC
                    ************************************************************

HsCD00002895        CAGGTCCACAAGAGCTTGCAGAGGATCCGGGAACGCAAGTTGGCCAACTTCATCCCGTGG
XM_005257222.1      CAGGTCCACAAGAGCCTGCAGAGGATCCGGGAACGGAAGTTGGCCAACTTCATCCCGTGG
NM_016437.2         CAGGTCCACAAGAGCCTGCAGAGGATCCGGGAACGGAAGTTGGCCAACTTCATCCCGTGG
NM_001070.4         CAGGTCCACAAGAGCTTGCAGAGGATCCGGGAACGCAAGTTGGCCAACTTCATCCCGTGG
                    *************** ******************* ************************

HsCD00002895        GGCCCCGCCAGCATCCAGGTGGCCCTGTCGAGGAAGTCTCCCTACCTGCCCTCGGCCCAC
XM_005257222.1      GGCCCCGCCAGCATCCAGGTGGCCCTGTCGAGGAAGTCTCCCTACCTGCCCTCGGCCCAC
NM_016437.2         GGCCCCGCCAGCATCCAGGTGGCCCTGTCGAGGAAGTCTCCCTACCTGCCCTCGGCCCAC
NM_001070.4         GGCCCCGCCAGCATCCAGGTGGCCCTGTCGAGGAAGTCTCCCTACCTGCCCTCGGCCCAC
                    ************************************************************

HsCD00002895        CGGGTCAGCGGGCTCATGATGGCCAACCACACCAGCATCTCCTCGCTCTTCGAGAGAACC
XM_005257222.1      CGGGTCAGCGGGCTCATGATGGCCAACCACACCAGCATCTCCTCGCTCTTTGAAAGTTCC
NM_016437.2         CGGGTCAGCGGGCTCATGATGGCCAACCACACCAGCATCTCCTCGCTCTTTGAAAGTTCC
NM_001070.4         CGGGTCAGCGGGCTCATGATGGCCAACCACACCAGCATCTCCTCGCTCTTCGAGAGAACC
                    ************************************************** **.**::**

HsCD00002895        TGTCGCCAGTATGACAAGCTGCGTAAGCGGGAGGCCTTCCTGGAGCAGTTCCGCAAGGAG
XM_005257222.1      TGCCAGCAGTTTGACAAGCTGCGGAAGCGGGATGCCTTCCTCGAGCAGTTCCGTAAGGAG
NM_016437.2         TGCCAGCAGTTTGACAAGCTGCGGAAGCGGGATGCCTTCCTCGAGCAGTTCCGTAAGGAG
NM_001070.4         TGTCGCCAGTATGACAAGCTGCGTAAGCGGGAGGCCTTCCTGGAGCAGTTCCGCAAGGAG
                    ** *. ****:************ ******** ******** *********** ******

HsCD00002895        GACATGTTCAAGGACAACTTTGATGAGATGGACACATCCAGGGAGATTGTGCAGCAGCTC
XM_005257222.1      GACATGTTCAAGGACAACTTTGATGAGATGGACAGGTCTAGGGAGGTTGTTCAGGAGCTC
NM_016437.2         GACATGTTCAAGGACAACTTTGATGAGATGGACAGGTCTAGGGAGGTTGTTCAGGAGCTC
NM_001070.4         GACATGTTCAAGGACAACTTTGATGAGATGGACACATCCAGGGAGATTGTGCAGCAGCTC
                    ********************************** .** ******.**** *** *****

HsCD00002895        ATCGATGAGTACCATGCGGCCACACGGCCAGACTACATCTCCTGGGGCACCCAGGAGCAG
XM_005257222.1      ATTGATGAGTACCATGCGGCCACCCAGCCAGACTACATTTCCTGGGGCACCCAGGAGCAG
NM_016437.2         ATTGATGAGTACCATGCGGCCACCCAGCCAGACTACATTTCCTGGGGCACCCAGGAGCAG
NM_001070.4         ATCGATGAGTACCATGCGGCCACACGGCCAGACTACATCTCCTGGGGCACCCAGGAGCAG
                    ** ********************.*.************ *********************

HsCD00002895        TTG
XM_005257222.1      TGA
NM_016437.2         TGA
NM_001070.4         TGA
                    * .

Pathways

NCI : PLK1 signaling events
Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Centrosome maturation
Reactome : G2/M Transition
Reactome : Loss of Nlp from mitotic centrosomes
Reactome : Loss of proteins required for interphase microtubule organizationÿ from the centrosome
Reactome : Mitotic G2-G2/M phases
Reactome : Recruitment of NuMA to mitotic centrosomes
Reactome : Recruitment of mitotic centrosome proteins and complexes
Reactome : Regulation of PLK1 Activity at G2/M Transition

Clone

Cloning Information : 27654
HIP Master Clone ID : 58030
Original Clone ID : FLH027654.01L

Organism


TAX_ID : 9606
Species Specific ID: 7283

Genome

Chromosome : 17
Map Location : 17q21
Ensembl : ENSG00000131462

Reagents

Labome : gamma-tubulin-antibody