DNASU Plasmid Repository • 480.965.5697 | Email

TM1403 (Thermotoga maritima) in pMH4 (bacterial expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  TM1403
Gene Name:  antibiotic ABC transporter ATP-binding protein
Sequence             
Original Clone ID: 14427
Keyword: None
Species: Thermotoga maritima
Type: cDNA
Vector Name: pMH4               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Joint Center for Structural Genomics
TmCD00289555

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 984nts         Open reading frame : 37 to 1020

ATGGGATCTGATAAAATTCATCATCATCATCATCACATGGAAGACATTATCGTCGTGGAA
AATCTGGTGAAAAAGTTCGGAGATTTCGAAGCGGTCAAAGGAGTCAGCTTCTCAGTGAAG
AAAGGAGAAATCTTCGCTTTTCTTGGTCCAAACGGTGCGGGAAAAACCACCACCATTCAC
ATGCTCACCACGCTTCTAAAACCGACGAGTGGAAAGGCATGGGTTGCCGGTCATGACGTG
CTGAAAGAACCTCGAGAGGTTAGAAGAAAAATCGGTATAGTCTTTCAGGATCAATCACTG
GACAGAGAGCTCACCGCGTATGAAAACATGTACATACACGGAAAAATATACGGCTACGGT
GGAGAAAAACTCAAAAAGAGAATCCTTGAACTTCTCGAGTTCGTGGAACTTCTGGAGTTC
AAAGACAAACCGGTCAAAACGTTCAGTGGTGGCATGGCAAGAAGACTCGAAATAGCTCGC
TCCTTGATACATGAACCAGAAGTTCTCTTTCTCGACGAACCCACTATTGGTCTCGATCCA
CACACAAGAGCGCATATGTGGGAATACATCTCGAAAATGAAAAAAGAACACAACATGACA
ATCTTTCTCACCACCCATTACATGGACGAGGCAGAACAGCTTGCTGACAGAGTGGCGATC
ATAGATCACGGGAAAATCATAGCACTCGGAACTCCAACCGAGTTGAAGAGAATGGTCGGA
AAAGAGATTATCTACGTGAGATTCTCTGAAGCCGTTGAATGTCTCGAAGGCGACTTCATA
GAGTCGTGTAGAAAACTCCCCGATGGAAGGCTGGAGCTGAATGTGGAAGATTCTGGAAGG
GCGATACCAAAGATTTTCGAACTGGCACAGCAGAAGGGTTTGAAAATAGAAGAAATCACC
TATCATAAACCCACGCTCAACGATGTTTTCCTCCATCTCACAGGAAGGGAGCTCAGAGAG
GAAGGACCGGAGAACTCTTTCAAAACCATGGCAAGAATGAGAATGAGGATGAGGAGGTAA
CGCGACTTAATTAACTCTAGATAACTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 984
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCAC

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 283264
Recommended expression in: HK100
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pMH4

pMH4 in advanced viewed

Synonyms: pMH-4
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enyzme (Pml I/Pac I) or PIPE cloning.
Comments: None
Size (bp): 5489
Parent Vector: None
Empty Vector: None
Properties: PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1 bacterial origin of replication 3331 4013
inducible trxn activator protein gene araC araC cds 5463 4585
negative selection marker ccdB ccdB negative selection gene (may be gone in with-insert form) 1576 1785
promoter T7 T7 promoter 290 310
promoter araC araC promoter 153 124
selectable marker CmR chloramphenicol resistance gene (CmR) 484 1140
selectable marker AmpR ampicillin resistance gene 2574 3233
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn termination sequence rrnB rrnB T2 transcription terminator 2238 2264

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism

Gene

 

Gene Symbol : TM1403
GENEID : 898073
Locus Tag : TM1403
Target GenBank: NP_229204

 

Protein

SMART domain : AAA : ATPases associated with a variety of cellular activities
Structural Annotation Wiki : TOPSAN
Swissprot ID : Q9X1C3

Clone

Cloning Information : 453815
HIP Master Clone ID : 383301
Original Clone ID : 14427

Experimental

TargetTrack - Experimental data : 283264

Organism


TAX_ID : 243274
Species Specific ID: 898073