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Detailed Plasmid Information

TOP

Explanation of Terms


Gene: Gene Symbol:  None
Gene Name:  None
Sequence             
Original Clone ID: 11178o2BCt5p1
Keyword: None
Species: Methanosarcina mazei Go1
Type: cDNA
Vector Name: pSGX3               Format:  CLOSED
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: C-terminal 6-His, TOPO
Mutation/
Discrepancy :
No / No
Publications: PMID: 23481913
Title: A continuous fluorescence assay for the characterization of Nudix hydrolases
PMID: 23481913
Title: A continuous fluorescence assay for the characterization of Nudix hydrolases
Authors: PSI
New York SGX Research Center for Structural Genomics
MmCD00291907

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 453nts         Open reading frame : 51 to 503

CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTGAGAAACCTT
ATATCATTTCCGTATATGCTCTCATCCGGAACGAAAAAGGGGAATTTCTGCTGCTCAGGC
GCTCGGAAAATTCCCGCACCAATGCAGGAAAGTGGGACCTTCCGGGAGGAAAGGTAAACC
CGGACGAGTCTCTTAAAGAAGGGGTTGCGCGTGAAGTCTGGGAGGAAACCGGAATTACAA
TGGTTCCCGGGGATATTGCAGGGCAGGTAAACTTTGAACTCACTGAAAAGAAGGTCATTG
CTATCGTGTTTGATGGGGGGTATGTTGTCGCTGACGTTAAATTGAGCTATGAGCACATTG
AATATTCCTGGGTCTCGCTGGAAAAGATTCTCGGTATGGAGACGCTCCCGGCTTATTTCC
GGGATTTCTTTGAAAGGTTTGATCGTGAGAACAAAAAACCTTCAAAGCTCTTTATTGAAG
GGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCA
CTCGAGCACCAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 453
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGTCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: SolubleProtein: Protein_Soluble
ProteinPurified: Protein_Purified
ProteinExpressed: Protein_Confirmed
Find experimental protocols in NYSGXRC-11178o
Recommended expression in: BL21(DE3)-Codon+RIL
       DyNA Vector Map

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Vector Name: pSGX3

pSGX3 in advanced viewed

Synonyms: pSGX-3, pSGX3_BC
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen
Size (bp): 5339
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start initiation start (Met, Ser, Leu) 5072 5079
TOPO recognition site TOPO TOPO cloning site 5076 5085
bacterial origin ori pBR322-type bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4983 5003
repressor protein gene LacI LacI CDS 3515 4531
selectable marker KanR kanamycin resistance gene 563 1375
tag His 6xHis tag 5086 5103
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 terminator 5231 5307

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Reagents

Gene

Target GenBank: NP_632944.1

 

Protein

PDB - Structural Annotation : 3GRN
Structural Annotation Wiki : TOPSAN
UniProt : Q8PYE2
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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Clone

Cloning Information : 484072
HIP Master Clone ID : 401800
Original Clone ID : 11178o2BCt5p1

Experimental

Structural Biology Knowledgebase : SBKB (3GRN)
TargetTrack - Experimental data : NYSGXRC-11178o

Reagents

Labome : MM_0920-cDNA