| Gene: |
Gene Symbol:
No insert Gene Name: No insert |
|---|---|
| Original Clone ID: | None |
| Type: | No insert |
| Vector Name: | pLIC-EGFP |
| Source: | Center for Structures of Membrane Proteins |
| Description: | Bacterial expression vector with C-terminal EGFP and 8x His tag separated from the insert by a TEV protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC) |
| Comments: | None |
| Publications: | None |
| Authors: |
Center for Structures of Membrane Proteins University of California San Francisco Joy Chen |
|
EvNO00292959 Price: Login for Pricing AVAILABLE Academic and non-profit labs Special MTA: PSI |
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
kanamycin Bacterial Selection Condition: 50ug/mL kanamycin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors. |
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| Vector Name: | pLIC-EGFP |
pLIC-EGFP in advanced viewed |
| Synonyms: | None | |
| Sequencing Primer: | Forward:
T7
Reverse: T7 terminator |
|
| Description: | Bacterial expression vector with C-terminal EGFP and 8x His tag separated from the insert by a TEV protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC) | |
| Comments: | None | |
| Size (bp): | 6077 | |
| Parent Vector: | None | |
| Properties: | bacterial expression | |
| Author Name: |
Center for Structures of Membrane Proteins University of California San Francisco Joy Chen |
|
| Publications: | None | |
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| phage origin | f1 | f1 phage origin | 0 | 0 |
| promoter | T7 | T7 promoter | 0 | 0 |
| protease cleavage site | TEV | TEV protease cleavage site | 0 | 0 |
| recombination site | LIC | LIC recombinational cloning site | 0 | 0 |
| selectable marker | KanR | Kanamycin resistance gene | 0 | 0 |
| tag | EGFP | C-terminal EGFP tag | 0 | 0 |
| tag | His | C-terminal 8xHis tag | 0 | 0 |
| trxn termination sequence | T7 term | T7 transcription termination site | 0 | 0 |
| Species Specific ID: | None |
|---|---|
| Target GenBank: | None |
