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Detailed Vector Information: pLIC-EGFP

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pLIC-EGFP              
Source: Center for Structures of Membrane Proteins
Description: Bacterial expression vector with C-terminal EGFP and 8x His tag separated from the insert by a TEV protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
Comments: None
Publications: None
Authors: Center for Structures of Membrane Proteins
University of California San Francisco
Joy Chen
EvNO00292959

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
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Vector Name: pLIC-EGFP

pLIC-EGFP in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with C-terminal EGFP and 8x His tag separated from the insert by a TEV protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
Comments: None
Size (bp): 6077
Parent Vector: None
Properties: bacterial expression
Author Name: Center for Structures of Membrane Proteins
University of California San Francisco
Joy Chen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
phage origin f1 f1 phage origin 0 0
promoter T7 T7 promoter 0 0
protease cleavage site TEV TEV protease cleavage site 0 0
recombination site LIC LIC recombinational cloning site 0 0
selectable marker KanR Kanamycin resistance gene 0 0
tag EGFP C-terminal EGFP tag 0 0
tag His C-terminal 8xHis tag 0 0
trxn termination sequence T7 term T7 transcription termination site 0 0

 

Species Specific ID: None
Target GenBank: None