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Ptprg (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Ptprg
Gene Name:  protein tyrosine phosphatase, receptor type, G
Sequence             
Original Clone ID: FLH481102.01X
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00294847

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 4329nts         Open reading frame : 35 to 4329

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCGGAGGTTACTGGAACCGTGTTG
GTGGATTTTGTTCCTGAAAATCACCAGTTCTGTGCTTCATTATGTGGTGTGCTTCCCGGC
ATTGACTGAAGGCTATGTGGGGACCCTGCAGGAGAGCAGACAGGACAGCTCAGTGCAGAT
CCGCAGACGAAAGGCATCCGGAGACCCATACTGGGCGTATTCTGGTGCCTATGGTCCTGA
GCACTGGGTCACATCTAGTGTCAGCTGCGGGGGCAGCCATCAGTCTCCTATAGACATTTT
AGACCACCATGCTCGCGTTGGCGATGAATACCAGGAGCTTCAACTTGATGGGTTTGACAA
CGAGTCCTCTAACAAAACCTGGATGAAAAATACAGGGAAAACAGTTGCCATCCTGCTGAA
AGACGATTATTTTGTGAGTGGTGCCGGACTGCCGGGCAGATTCAAAGCTGAGAAGGTGGA
GTTTCACTGGGGCCACAGCAATGGCTCCGCTGGCTCAGAGCATAGTGTCAATGGCCGGAG
GTTTCCTGTGGAGATGCAGATTTTCTTTTACAACCCAGACGACTTTGACAGCTTTCAAAC
GGCAATTTCTGAGAACAGAATAATTGGAGCTATGGCCATATTTTTCCAAGTCAGTCCGAG
GGACAATTCTGCACTGGATCCTATTATCCACGGGCTGAAGGGCGTCGTACATCACGAGAA
GGAGACTTTCCTGGATCCTTTCATTCTCCGAGACCTCCTGCCCGCATCCCTGGGGAGTTA
TTACAGGTACACAGGCTCCTTGACTACGCCACCCTGTAGTGAAATAGTGGAGTGGATTGT
CTTCCGGAGGCCCGTTCCCATCTCCTATCACCAGCTTGAGGCTTTTTATTCTATCTTCAC
CACGGAGCAGCAAGACCATGTCAAGTCAGTGGAGTACCTGAGAAATAACTTCCGACCACA
ACAAGCTCTGAATGACAGAGTGGTGTCAAAGTCTGCTGTCCGGGATGCCTGGAACCATGA
CCTGGCAGACTTCTTGGACAACCCACTAGGCACAGAAGCCTCTAAAGTTTGCAGCTCTCC
GCCCATCCACATGAAGGTGCAGCCTCTGAACCAGACAGCTCTGCAGGTGTCCTGGAGCCA
ACCTGAAACCATCTACCACCCGCCCATCATGAATTACATGATCTCCTATAGCTGGACCAA
GAATGAGGACGAGAAGGAGAAGACATTTACAAAAGACAGTGACAAGGACTTGAAAGCCAC
CATCAGCCATGTCTCACCCGATAGCCTTTACCTATTCCGAGTACAGGCTGTGTGTAGGAA
TGACATGCGGAGTGACTTCAGCCAGACAATGCTCTTTCAAGCTAATACTACCCGGATCTT
CCAAGGGACCAGGATTGTGAAAACAGGAGTGCCGACAGCCTCTCCTGCCTCTTCAGCAGA
CATGGCCCCCATCAGCTCTGGCTCTTCCACTTGGACATCCTCCGGCATCCCCTTCTCATT
TGTTTCCATGGCAACTGGGATGGGCCCTTCCTCCAGTGGCAGCCAAGCTACAGTGGCCTC
TGTGGTCACCAGCACACTGTTGGCAGGCCTGGGCTTCGGTGGTGGTGGCATCTCCTCCTT
CCCCAGTACCGTGTGGCCCACGCGCCTTCCCACCGCATCTGCAGCCAGCAAGCAGGCAGG
GAGGACAGTTCTTGCCACCACCGAGGCCTTAGCTTCCCCTGGGCCGGATGTCGACTCGGC
ACCGACGAAGGACAGCGAGGGTACAGAGGAGGGAGAGAAGGAGGAGAAAAGCGAGAGCGA
GGATGGTGAGCGAGAGCATGAGGAGGAAGAGAAGGACTCTGAGAAGAAGGAGAAGAGTGA
GGCTACTCACACAGCCGCAGAGAGTGACCGGACTGCACCTGCCCCCACCCCCTCTTCCCC
TCATAGGACTGCAGCGGAGGGAGGACATCAGACTATACCTGGGCGCAGGCAGGACCACTC
TGCCCCTGCCACAGACCAGCCGGGCCACGTTGCCCCAGACTTGGATCCCCTTGTGGACAC
AGCCACCCAAGTGCCCCCAACAGCCACAGAGGAACATTATTCAGGGAGTGATCCCAGGAG
GCCCGAAATGCCATCTAAAAAGCCTATGTCCCGAGGGGACCGATTTTCTGAGGATAGCAA
ATTCATCACAGTTAACCCAGCTGAGAAGAACACCTCTGGCATGCTAAGCCGCCCCTCCCC
CGGGAGGATGGAGTGGATCATCCCCCTGATTGTGGTCTCAGCCTTGACCTTCGTGTGCCT
CGTCCTCCTCATTGCCGTGCTGGTGTACTGGAGAGGGTGTAACAAAATAAAGTCCAAGGG
CTTTCCCCGACGTTCCCGTGAAGTGCCTTCTTCTGGGGAGAGAGGAGAGAAGGGGAGCAG
AAAATGTTTTCAGACTGCTCATTTCTATGTGGAAGACAGCAGTTCACCTCGGGTGGTCCC
CAACGAAAGTGTTCCTATTATTCCCATCCCGGATGACATGGAAGCCATTCCTGTCAAACA
GTTTGGTAAACACATCGGTGAGCTCTATTCCAATAGCCAGCATGGCTTCTCGGAAGATTT
TGAGGAAGTCCAGCGCTGTACAGCTGATATGAACATCACTGCAGAGCATTCCAATCATCC
GGATAACAAGCACAAAAACAGATACATCAACATTTTAGCATATGATCACAGTAGGGTGAA
GTTAAGACCTTTACCAGGAAAGGACTCTAAGCACAGCGACTACATTAATGCAAACTATGT
CGATGGTTACAACAAAGCGAAAGCCTACATTGCCACCCAGGGACCTTTAAAATCCACCTT
TGAAGACTTCTGGAGGATGATCTGGGAACAAAACACGGGAATCATCATCATGATCACGAA
CCTCGTGGAGAAAGGAAGGAGAAAGTGTGATCAGTATTGGCCAACGGAGAACACTGAGGA
GTACGGGAACATTATTGTCACACTGAAGAGCACAAAAGTACATGCCTGCTACACCGTCCG
TCGTCTTTCAGTTAGAAACACAAAAGTGAAAAAGGGCCAGAAGGGCAACCCCAAAGGTCG
TCAGAATGAGCGGACGGTGATCCAGTACCACTACACACAGTGGCCTGACATGGGTGTCCC
TGAGTACGCCCTCCCAGTCCTGACCTTCGTGAGGAGGTCATCTGCGGCCCGCATGCCAGA
CATGGGGCCCGTGTTGGTGCACTGCAGCGCTGGTGTGGGCAGGACAGGCACCTACATTGT
AATAGACAGCATGCTGCAGCAGATCAAAGACAAAAGCACAGTTAATGTCCTGGGCTTCCT
GAAGCATATCAGGACACAGAGGAACTACCTCGTCCAGACTGAGGAGCAGTACATTTTCAT
CCATGATGCCTTGTTGGAAGCCATTCTCGGGAAGGAAACTGAAGTATCTTCAAGTCAGCT
GCATAGCTATGTTAACAGCATCCTCATACCAGGAGTAGGAGGAAAGACACGGCTGGAAAA
GCAGTTTAAGCTGATCACGCAGTGTAATGCAAAGTATGTGGAGTGCTTCAGTGCCCAGAA
GGAGTGCAACAAAGAGAAGAACAGGAACTCGTCTGTTGTGCCAGCTGAGCGTGCTCGAGT
GGGACTTGCACCGTTGCCCGGGATGAAAGGAACAGATTACATTAATGCTTCTTACATCAT
GGGGTATTATAGAAGCAACGAGTTCATCATAACCCAGCATCCTCTGCCACACACTACGAA
AGATTTCTGGAGAATGATTTGGGATCATAATGCACAGATCATTGTCATGCTGCCAGACAA
CCAGAGCCTGGCAGAAGATGAGTTTGTGTACTGGCCGAGTCGTGAGGAATCCATGAACTG
TGAGGCCTTTACTGTCACCCTCATCAGCAAAGACAGGCTATGCCTCTCGAATGAAGAACA
AATTATCATCCATGACTTCATCCTTGAAGCTACACAGGATGACTACGTACTAGAGGTTCG
GCACTTTCAGTGTCCCAAATGGCCTAACCCAGATGCCCCTATAAGTAGTACCTTCGAACT
GATCAATGTCATCAAGGAAGAGGCCTTAACCCGGGATGGCCCCACCATTGTTCATGATGA
GTATGGAGCAGTTTCTGCAGGAATGTTGTGTGCCCTTACCACCTTGTCCCAGCAGCTAGA
GAATGAAAATGCTGTGGATGTGTTCCAGGTGGCAAAAATGATCAATCTTATGAGACCTGG
AGTATTCACAGACATTGAACAATACCAGTTTGTCTACAAAGCAATGCTCAGCTTGATCAG
TACTAAAGAAAATGGAAATGGTCCCATGACCGGGGACAAAAACGGTGCTGTGCTAACTGC
AGAGGAATCAGACCCTGCAGAGAGCATGGAGTCTCTGGTGTAGGGCCTCATGGGCCCAGC
TTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 5024
Start on reference sequence 696
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Ptprg
Symbol Nomenclature : Ptprg
SYNONYM : 5430405N12Rik; AW046354; AW549872; RPTPgamma
Designation : Protein-tyrosine phosphatase gamma precursor (R-PTP-gamma)|R-PTP-gamma|receptor-type tyrosine-protein phosphatase gamma
Full Nomenclature : protein tyrosine phosphatase, receptor type, G
GENEID : 19270
GI : 195934816
GenBank Accession : BC168385
Mouse Genome Informatics : 97814
Vega : OTTMUSG00000029429
Target GenBank: NM_008981.2

 

Clone

Cloning Information : 481102
HIP Master Clone ID : 398830
IMAGE ID : 100068354
Original Clone ID : FLH481102.01X

Organism


TAX_ID : 10090
Species Specific ID: 19270

Genome

Chromosome : 14
Map Location : 14 A2|14 6.33 cM
Ensembl : ENSMUSG00000021745

Reagents

Labome : Ptprg-antibody