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Iqsec3 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Iqsec3
Gene Name:  IQ motif and Sec7 domain 3
Sequence             
Original Clone ID: FLH481105.01X
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00294850

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 3588nts         Open reading frame : 35 to 3588

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAGAGCCTGCTGGAGAACCCGGT
GCGCGCCGTGCTCTACCTCAAGGAGCTCACGGCCATCGTGCAGAACCAGCAAAGCCTCAT
CCATACCCAGCGCCAGCGCATCGATGAGCTGGAGCGGCGTCTAGACGAGCTGAGCGCGGA
GAACCGCAGCCTGTGGGAACACCAGCAGTTGCTGCAAGCCCAGCCTCCGCCTGGGCTGGT
TCCCCCGCCGCCGTCTGCCCCACTGCCTGCTCCTGCAGTCACTGCTCCGGCCGCCGCTGC
GGCCCAGGAACCGCTTCAGGACCACGGACAGCTCATACCCGCTTCACCGGAGCCGCCGCT
TCAGCACCACGGTCAGCTCCTGGCGCAGCCCCAACCTGCACCCAGCAGCAGGGTTCAGAC
TCCTCAGTCGCCCCACCAGCACCCGGTGGCACCCGGGGCCATAGCTGACAAGGAGAAGGA
GCGTCCCTCGAGTTGTTGTGCTGCCGCGGGAGCCCTGCTTCAACACGCATCCCCCGCCGC
CCTCGGCAAGGGCGTCCTGAGCAGGAGACCCGAGAATGAGACTGTGCTACACCAATTCTG
CTGCCCTGCCGCTGACACCGAGCAGAAGCCTGCCTGCTCCGACCTGGCTTCCCAAAGTGA
TGGCTCCTGTGCCCAGGCCGGTGGGGGCATGGAGGACTCCGTGGTGGCAGCGGTGGCAGC
CGGCAGACCCAGTGCCCATGCCCCGAAGGCTCAAGCCCCGGAGCTACAGCAGGAGGAGGA
GCGGCCAGGGGCAGTGGGCTCCCCACGGGCTGGCCCCCTCCGCGCGGCCTCTCCTGGCCG
GCAGCAGCCTGCCCTGGCGACGGCGCTCTGCTCCCACACTCCTGCTGCCTCCGAATACGA
ACTCTCCCTTGACCTAAAGAATAAACAGATTGAAATGCTAGAACACAAATACGGGGGCCA
CCTGGTATCCCGACGCGCCGCGTGCACCATTCAAACCGCTTTTCGCCAGTACCAGCTCAG
CAAGAACTTTGAGAAGATCCGTAACTCGCTCCTGGAGAGCCGCCTGCCTCGGCGGATCTC
ACTGCGCAAGGTGAGGGCGCCCACAGCGGAGAGTCTGGTGGCAGAGAAGGCGCTCCTGGA
GGGCTGTGGGCTCCTTGGGCTCCCGCTGGGGCGCTCGCCCTCGCTGCCGCCCACCTTCGC
TGGCTCGCTCACCGAGCTGGAGGACTCCTTTACCGAGCAGGTGCAGTCCCTGGCCAAGTC
CATCGACGACGCCCTCAGCACCTGGAGCCTCAAGACCATGTGCTCGCTGCAAGAGAGTGG
CGCGTACCAGCTCCACCAGGCGCTGCACCCGAGTGCGGGACAGCCAGGCCTGGAGACCGA
GGCGGCGGCGCGGGAGCCAGAGAGCGGCCCCGGGTCTGGAGATGAGGCCGGTGGTCTGCC
TCAGGGTCACAGCGGTACCCTCATGATGGCTTTCCGGGACGTTACGGTGCAGATCGCTAA
CCAGAACATCTCTGTTTCCTCCTCCACCGCCCTGTCTGTGGCCAACTGTCTGGGTGCACA
GACCGCCCAGGCCACAGCCGAGCCTGCTGCCGCCCAAGCCGAACAAGAGGACACCGCGGA
TCAGGAGGTCTCGGAAGTCCCAGCCTCGGAACAGATGGACCCACCCAGTGAGGACTCAGA
GGCAGCGGAGAGCCGCGCCCAGAGCGCACAGGAACCCGCGGTGGCACAGGCCGTGGTGGA
GGAGGCGGTGGCTACAGAGGCAGAGGAGGAAGAGGAGGGGGCCAAACAGGCAGGGAAGGG
GGCAGAGGCCGAGGGAGGCGACAACTCTGAGCAGCTGAGCAGCAGCAGCGCTTCCACCAA
GTCTGCCAAATCCAGCTCAGAGGCTTCTGCCGCTGCCTCCAAGGAGGCCCTGCAGGCTGT
GATCCTGAGCTTGCCCCGCTATCACTGCGAGAACCCAGCCAGCTGCAGGTCCCCCACGCT
CTCCACAGACACCCTGCGCAAGCGGCTGTACCGCATCGGCCTCAACCTCTTCAACATAAA
TCCGGACAAGGGCATCCAGTTCCTGATCTCACGTGGCTTCATCCCTGACACCCCCATCGG
TGTGGCCCACTTCCTCCTCCAGCGTAAAGGCCTCAGCCGCCAGATGATAGGAGAGTTCCT
GGGCAACAGCAAGAAGCAGTTCAACCGGGACGTGCTGGACTGTGTGGTAGATGAGATGGA
CTTTTCCAACATGGAGCTGGATGAAGCCCTGAGGAAGTTTCAAGCCCACATCCGTGTGCA
GGGGGAAGCACAGAAGGTGGAGCGACTCATAGAAGCTTTCAGCCAGCGCTACTGCATGTG
CAATCCTGAGGTGGTGCAACAGTTCCACAACCCAGACACCATCTTCATCCTGGCCTTTGC
CATCATCCTTCTCAACACCGACATGTACAGCCCCAACATCAAGCCTGACCGGAAGATGAT
GCTAGAAGACTTTATCCGGAACCTCCGAGGTGTGGATGATGGTGCAGACATCCCCAGAGA
ACTGGTGGTAGGCATCTATGAGAGGATCCAGCAAAAGGAGCTCAAATCCAATGAGGACCA
TGTCACATATGTCACCAAGGTGGAGAAGTCCATCGTGGGCATGAAGACGGTGCTGTCAAT
GCCACACCGTCGCCTGGTCTGCTGTAGCCGGCTCTTCGAGGTGACCGATGTGAACAAGCT
ACAGAAACAAGCAGCACACCAGAGAGAAGTGTTCCTCTTCAATGATCTGCTTGTGATTCT
CAAGCTATGCCCAAAGAAGAAGAGCTCCTTCACGTACACCTTCTGCAAGGCAGTCGGCCT
GTTAGGCATGCGCTTTCACCTCTTCGAGAACGAGTATTACTCCCATGGCATCACCCTGGC
AACCCCACTCTCGGGCTCCGAGAAGAAGCAGGTGCTGCACTTCTGTGCCCTGGGCTCCGA
CGAGATGCAGAAGTTCGTGGAAGACCTGAAGGAGTCCATCGCTGAGGTGACAGAGCTGGA
GCAGATCCGGATAGAATGGGAACTGGAGAAGCAGCAGGGCACAAAGACTCTCTCCGTAAG
GTCTGCTGGAGCCCAAGGGGACCCACAGTCGAAGCAAGGATCACCCACAGCCAAGAGGGA
GGCGATGGCAGGAGAGAAAGCGGCGGAGAGCTCGGGGGAGGTGTCAATTCACAACAGGCT
TCAAACGTCCCAGCACAGCCCCAAGTTGGGGGTCGAGAGGGGAGCGCCTGCACCATCACC
ACCAACATCACCACCGCCGTTGCCGCCAGACCCACAGCCTAGCCCCCTGAGGGAGCAGCC
CCCACCGCTGCCACTGCCACCACCCACACCTCCAGGCACCCTGGTACAGTGCCAGCAGAT
TGTCAAGGTCATTGTCCTGGACAAACCCTGCTTGGCCCGCATGGAGCCCCTGCTGAGCCA
GGCTCTGTCCTGTTACGCCTCATCCTCCTCAGACTCCTGTGGCTCCACACCCTTGCGTGG
TCCAGGCTCCCCAGTTAAGGTCATCCACCAGCCTCCACTGCCCCCGCCCCCGCCCCCATA
CAACCACCCTCATCAGTTCTGCCCCCCAGGCTCCATGCTGCTCCGGCGCCGTTACTCCAG
CGGCTCCAGGAGCCTGGTGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3703
Start on reference sequence 116
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Iqsec3
Symbol Nomenclature : Iqsec3
SYNONYM : AK122446; BRAG3; mKIAA1110; synarfGEF
Designation : IQ motif and SEC7 domain-containing protein 3
Full Nomenclature : IQ motif and Sec7 domain 3
GENEID : 243621
GI : 195934820
GenBank Accession : BC168388
Mouse Genome Informatics : 2677208
Vega : OTTMUSG00000023463
Target GenBank: NM_001033354.2

 

Protein

SMART domain : Sec7 : Sec7 domain
UniProt : Q3TES0

Clone

Cloning Information : 481105
HIP Master Clone ID : 398833
IMAGE ID : 100068357
Original Clone ID : FLH481105.01X

Organism


TAX_ID : 10090
Species Specific ID: 243621

Genome

Chromosome : 6
Map Location : 6 F1|6
Ensembl : ENSMUSG00000040797

Reagents

Labome : Iqsec3-antibody