Gene:
Gene Symbol:
NRG2
Gene Name:
neuregulin 2
Original Clone ID:
FLH481135.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294880
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCGGCAGGTTTGCTGCTCAGCGCTGCCGCCGCCGCCACTGGAGAAGGGTCGGTGCAGC
AGCTACAGCGACAGCAGCAGCAGCAGCAGCGAGAGGAGCAGCAGCAGCAGCAGCAGCAGC
AGCGAGAGCGGCAGCAGCAGCAGGAGCAGCAGCAACAACAGCAGCATCTCTCGTCCCGCT
GCGCCCCCAGAGCCGCGGCCGCAGCAACAGCCGCAGCCCCGCAGCCCCGCAGCCCGGAGA
GCCGCCGCCCGTTCGCGAGCCGCAGCCGCCGGCGGCATGAGGCGCGACCCGGCCCCCGGC
TTCTCCATGCTGCTCTTCGGTGTGTCGCTCGCCTGCTACTCGCCCAGCCTCAAGTCAGTG
CAGGACCAGGCGTACAAGGCACCCGTGGTGGTGGAGGGCAAGGTACAGGGGCTGGTCCCA
GCCGGCGGCTCCAGCTCCAACAGCACCCGAGAGCCGCCCGCCTCGGGTCGGGTGGCGTTG
GTAAAGGTGCTGGACAAGTGGCCGCTCCGGAGCGGGGGGCTGCAGCGCGAGCAGGTGATC
AGCGTGGGCTCCTGTGTGCCGCTCGAAAGGAACCAGCGCTACATCTTTTTCCTGGAGCCC
ACGGAACAGCCCTTAGTCTTTAAGACGGCCTTTGCCCCCCTCGATACCAACGGCAAAAAT
CTCAAGAAAGAGGTGGGCAAGATCCTGTGCACTGACTGCGCCACCCGGCCCAAGTTGAAG
AAGATGAAGAGCCAGACGGGACAGGTGGGTGAGAAGCAATCGCTGAAGTGTGAGGCAGCA
GCCGGTAATCCCCAGCCTTCCTACCGTTGGTTCAAGGATGGCAAGGAGCTCAACCGCAGC
CGAGACATTCGCATCAAATATGGCAACGGCAGAAAGAACTCACGACTACAGTTCAACAAG
GTGAAGGTGGAGGACGCTGGGGAGTATGTCTGCGAGGCCGAGAACATCCTGGGGAAGGAC
ACCGTCCGGGGCCGGCTTTACGTCAACAGCGTGAGCACCACCCTGTCATCCTGGTCGGGG
CACGCCCGGAAGTGCAACGAGACAGCCAAGTCCTATTGCGTCAATGGAGGCGTCTGCTAC
TACATCGAGGGCATCAACCAGCTCTCCTGCAAGTGTCCTGTGGGATACACCGGGGACAGG
TGTCAGCAGTTCGCAATGGTCAACTTCTCCAAGCACCTTGGATTTGAATTAAAGGAAGCC
GAGGAGCTGTACCAGAAGAGGGTCCTGACCATCACGGGCATCTGCGTGGCTCTGCTGGTC
GTGGGCATCGTCTGTGTGGTGGCCTACTGCAAGACCAAAAAACAGCGGAAGCAGATGCAC
AACCACCTCCGGCAGAACATGTGCCCGGCCCATCAGAACCGGAGCTTGGCCAATGGGCCC
AGCCACCCCCGGCTGGACCCAGAGGAGATCCAGATGGCAGATTATATTTCCAAGAACGTG
CCAGCCACAGACCATGTCATCAGGAGAGAAACTGAGACCACCTTCTCTGGGAGCCACTCC
TGTTCTCCTTCTCACCACTGCTCCACAGCCACACCCACCTCCAGCCACAGACACGAGAGC
CACACGTGGAGCCTGGAACGTTCTGAGAGCCTGACTTCTGACTCCCAGTCGGGGATCATG
CTATCATCAGTGGGTACCAGCAAATGCAACAGCCCAGCATGTGTGGAGGCCCGGGCAAGG
CGGGCAGCAGCCTACAACCTGGAGGAGCGGCGCAGGGCCACCGCGCCACCCTATCACGAT
TCCGTGGACTCCCTTCGCGACTCCCCACACAGCGAGAGGTACGTGTCGGCCCTGACCACG
CCCGCGCGCCTCTCGCCCGTGGACTTCCACTACTCGCTGGCCACGCAGGTGCCAACTTTC
GAGATCACGTCCCCCAACTCGGCGCACGCCGTGTCGCTGCCGCCGGCGGCGCCCATCAGT
TACCGCCTGGCCGAGCAGCAGCCGTTACTGCGGCACCCGGCGCCCCCCGGCCCGGGACCC
GGACCCGGGCCCGGGCCCGGGCCCGGCGCAGACATGCAGCGCAGCTATGACAGCTACTAT
TACCCCGCGGCGGGGCCCGGACCGCGGCGCGGGACCTGCGCGCTCGGCGGCAGCCTGGGC
AGCCTGCCTGCCAGCCCCTTCCGCATCCCCGAGGACGACGAGTACGAGACCACGCAGGAG
TGCGCGCCCCCGCCGCCGCCGCGGCCGCGCGCGCGCGGTGCGTCCCGCAGGACGTCGGCG
GGGCCCCGGCGCTGGCGCCGCTCGCGCCTCAACGGGCTGGCGGCGCAGCGCGCACGGGCG
GCGAGGGACTCGCTGTCGCTGAGCAGCGGCTCGGGCGGCGGCTCAGCCTCGGCGTCGGAC
GACGACGCGGACGACGCGGACGGGGCGCTGGCGGCCGAGAGCACACCTTTCCTGGGCCTG
CGTGGGGCGCACGACGCGCTGCGCTCGGACTCGCCGCCACTGTGCCCGGCGGCCGACAGC
AGGACTTACTACTCACTGGACAGCCACAGCACGCGGGCCAGCAGCAGACACAGCCGCGGG
CCGCCCCCGCGGGCCAAGCAGGACTCGGCGCCACTC
Protein sequence predicted based on nucleotide sequence
MRQVCCSALPPPPLEKGRCSSYSDSSSSSSERSSSSSSSSSESGSSSRSSSNNSSISRPA APPEPRPQQQPQPRSPAARRAAARSRAAAAGGMRRDPAPGFSMLLFGVSLACYSPSLKSV QDQAYKAPVVVEGKVQGLVPAGGSSSNSTREPPASGRVALVKVLDKWPLRSGGLQREQVI SVGSCVPLERNQRYIFFLEPTEQPLVFKTAFAPLDTNGKNLKKEVGKILCTDCATRPKLK KMKSQTGQVGEKQSLKCEAAAGNPQPSYRWFKDGKELNRSRDIRIKYGNGRKNSRLQFNK VKVEDAGEYVCEAENILGKDTVRGRLYVNSVSTTLSSWSGHARKCNETAKSYCVNGGVCY YIEGINQLSCKCPVGYTGDRCQQFAMVNFSKHLGFELKEAEELYQKRVLTITGICVALLV VGIVCVVAYCKTKKQRKQMHNHLRQNMCPAHQNRSLANGPSHPRLDPEEIQMADYISKNV PATDHVIRRETETTFSGSHSCSPSHHCSTATPTSSHRHESHTWSLERSESLTSDSQSGIM LSSVGTSKCNSPACVEARARRAAAYNLEERRRATAPPYHDSVDSLRDSPHSERYVSALTT PARLSPVDFHYSLATQVPTFEITSPNSAHAVSLPPAAPISYRLAEQQPLLRHPAPPGPGP GPGPGPGPGADMQRSYDSYYYPAAGPGPRRGTCALGGSLGSLPASPFRIPEDDEYETTQE CAPPPPPRPRARGASRRTSAGPRRWRRSRLNGLAAQRARAARDSLSLSSGSGGGSASASD DDADDADGALAAESTPFLGLRGAHDALRSDSPPLCPAADSRTYYSLDSHSTRASSRHSRG PPPRAKQDSAPL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
226
End on reference sequence
2784
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
