RGS12 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  RGS12
Gene Name:  regulator of G-protein signaling 12
Original Clone ID:
FLH481402.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295147
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4341nts
Open reading frame: 1 to 4341

ATGTTTAGAGCTGGGGAGGCCTCCAAACGCCCATTGCCTGGGCCGTCGCCCCCAAGGGTG
CGGAGTGTGGAGGTTGCCCGGGGGAGGGCCGGCTACGGATTCACGCTTTCGGGACAGGCA
CCCTGTGTGCTCAGCTGCGTCATGAGAGGGAGCCCTGCGGATTTCGTGGGCCTCCGAGCT
GGAGACCAGATACTTGCTGTCAATGAAATCAACGTGAAAAAAGCATCTCATGAAGATGTA
GTGAAATTAATTGGGAAGTGCTCTGGTGTCCTTCACATGGTGATTGCTGAAGGCGTCGGC
CGCTTCGAATCCTGTTCCAGTGATGAAGAAGGGGGACTCTATGAAGGAAAAGGCTGGCTG
AAGCCCAAGCTGGATTCTAAAGCACTAGGTATAAACAGAGCAGAGCGAGTCGTGGAGGAA
ATGCAGTCTGGTGGAATTTTCAATATGATTTTTGAAAACCCGAGCCTTTGTGCGAGCAAT
TCAGAGCCCTTGAAATTGAAACAAAGATCCCTTTCAGAGTCGGCCGCAACTCGATTTGAT
GTTGGACATGAAAGTATAAATAATCCAAATCCCAACATGCTTTCTAAGGAGGAAATATCA
AAAGTTATTCATGATGATTCGGTTTTCAGCATTGGACTAGAAAGTCATGACGATTTTGCA
TTGGATGCAAGTATTTTAAACGTGGCGATGATCGTGGGCTACTTAGGCTCCATTGAGCTT
CCTTCCACGAGCTCCAACCTGGAGTCCGACAGCTTGCAAGCCATCCGCGGCTGCATGCGG
CGCCTGCGGGCAGAGCAGAAAATCCACTCGCTGGTGACCATGAAGATCATGCACGACTGT
GTGCAGCTGAGCACTGACAAGGCTGGAGTCGTGGCCGAGTACCCGGCCGAGAAGCTGGCC
TTCAGCGCCGTGTGCCCGGACGACCGGCGATTTTTCGGGTTGGTTACCATGCAGACGAAT
GACGACGGGAGCCTGGCCCAGGAGGAGGAGGGCGCCCTGCGGACTTCCTGCCACGTGTTC
ATGGTGGACCCAGACTTGTTTAATCACAAGATCCACCAAGGCATTGCTCGGCGGTTTGGG
TTTGAGTGCACGGCCGACCCAGACACCAATGGCTGTCTGGAATTCCCGGCGTCCTCCCTC
CCCGTCCTGCAGTTCATCTCTGTCCTGTACCGAGACATGGGTGAGCTGATTGAGGGCATG
CGGGCCCGCGCCTTTCTGGACGGGGACGCCGATGCCCACCAGAACAACAGCACCAGCAGC
AACAGTGACAGCGGCATTGGGAACTTCCACCAGGAGGAGAAGAGCAACCGGGTCCTTGTG
GTGGACCTGGGTGGGAGCTCGAGCAGACACGGCCCCGGAGGCAGCGCGTGGGACGGTGTG
GGTGGGAGGGGTGCCCAGCCCTGGGGTGCTCCCTGGACTGGGCCCTTCTGTCCGGACCCC
GAAGGGAGCCCCCCATTTGAGGCCGCTCATCAGACTGACAGGTTCTGGGACCTAAACAAG
CACCTAGGGCCAGCCTCTCCTGTGGAGGTGCCCCCAGCTTCCTTGAGGAGCTCAGTCCCC
CCTTCCAAGAGGGGCACCGTGGGTGCTGGCTGTGGTTTCAACCAGCGCTGGCTCCCGGTC
CACGTGCTCCGGGAGTGGCAGTGCGGACACACCAGCGACCAGGACTCTTACACAGATTCC
ACCGATGGCTGGTCCAGCATCAACTGCGGCACACTGCCCCCTCCTATGAGCAAGATCCCC
GCAGACCGCTACAGGGTGGAGGGCAGCTTCGCGCAGCCCCCGCTGAATGCCCCGAAGAGG
GAGTGGTCCAGGAAGGCCTTTGGAATGCAAAGCATTTTTGGTCCCCATCGAAATGTTCGA
AAGACTAAGGAAGATAAAAAGGGCTCAAAATTTGGGCGGGGAACTGGACTCACTCAGCCT
TCTCAACGCACGTCTGCTCGGAGATCATTTGGGAGATCCAAGAGATTCAGTATCACTCGC
TCCCTTGATGATCTTGAGTCTGCAACTGTGTCTGATGGCGAGTTGACGGGCGCCGACCTG
AAGGACTGCGTCAGCAACAACAGCCTGAGCAGCAATGCCAGCCTCCCCAGCGTGCAGAGC
TGCCGGCGCCTGCGTGAGAGGAGGGTCGCCAGCTGGGCCGTGTCCTTTGAGCGCCTGCTG
CAGGACCCCGTCGGTGTCCGCTACTTCTCTGATTTTCTAAGGAAAGAATTCAGTGAAGAA
AACATTTTATTCTGGCAGGCCTGTGAATATTTTAATCATGTTCCTGCACATGACAAAAAG
GAGCTTTCCTACAGGGCCCGGGAGATTTTCAGTAAGTTTCTCTGCAGCAAAGCCACCACC
CCGGTCAACATCGACAGCCAGGCCCAGCTAGCAGACGACGTCCTCCGCGCACCTCACCCA
GACATGTTCAAGGAGCAGCAGCTGCAGATCTTCAATCTCATGAAGTTTGATAGCTACACT
CGCTTTCTGAAGTCCCCGCTGTACCAGGAATGCATCCTGGCGGAAGTGGAGGGCCGTGCA
CTCCCGGACTCGCAGCAGGTCCCCAGCAGCCCGGCTTCCAAGCACAGCCTCGGTTCAGAC
CACTCCAGTGTGTCCACGCCAAAAAAGTTAAGTGGAAAATCAAAATCCGGCCGATCCCTG
AATGAAGAGCTGGGGGATGAGGACAGCGAGAAGAAGCGGAAAGGCGCGTTTTTCTCGTGG
TCGCGGACCAGGAGCACCGGGAGGTCCCAGAAAAAGAGGGAGCACGGGGACCACGCAGAC
GACGCCCTGCATGCCAATGGAGGCCTGTGTCGCCGAGAGTCGCAGGGCTCTGTGTCCTCT
GCGGGGAGCCTGGACCTGTCGGAGGCCTGCAGGACTTTGGCACCCGAGAAGGACAAGGCC
ACCAAGCACTGCTGCATTCATCTCCCGGATGGGACATCCTGCGTGGTGGCTGTCAAGGCG
GGCTTCTCCATCAAAGACATCCTGTCCGGACTCTGTGAGCGGCATGGCATCAACGGGGCG
GCCGCGGACCTCTTCCTGGTGGGCGGGGACAAGCCTCTGGTGCTGCACCAAGACAGTAGC
ATCTTGGAGTCAAGGGACCTGCGCCTAGAAAAGCGCACCTTGTTTCGGCTGGATCTTGTT
CCGATTAACCGGTCAGTGGGACTCAAGGCCAAGCCCACCAAGCCCGTCACGGAGGTGCTG
CGGCCCGTGGTGGCCAGATACGGCCTGGACCTCAGTGGCCTGCTGGTGAGGCTGAGTGGA
GAGAAGGAGCCCCTGGACCTTGGCGCCCCTATATCGAGTCTGGACGGACAGCGGGTTGTC
TTGGAGGAGAAGGATCCTTCCAGAGGAAAGGCATCCGCAGATAAACAGAAAGGTGTGCCA
GTGAAACAGAACACAGCTGTAAATTCCAGCTCCAGAAACCACTCGGCTACGGGAGAGGAA
AGAACACTAGGCAAGTCTAATTCTATTAAAATAAAAGGAGAAAATGGAAAAAATGCTAGG
GATCCCCGGCTTTCAAAGAGAGAAGAATCTATTGCAAAGATTGGGAAAAAAAAATATCAG
AAAATTAATTTGGACGAAGCAGAGGAGTTTTTTGAGCTTATTTCCAAAGCTCAGAGCAAC
AGAGCAGATGACCAACGTGGGCTGCTAAGGAAGGAAGACCTGGTGTTGCCAGAGTTCCTC
CGTTTACCTCCTGGTTCCACAGAACTCACCCTCCCCACTCCAGCTGCTGTGGCCAAGGGC
TTTAGCAAGAGAAGCGCCACAGGCAACGGCCGGGAGAGCGCCTCCCAGCCTGGCGAGCAG
TGGGAGCCAGTCCAGGAGAGCAGCGACAGCCCGTCCACCAGCCCGGGCTCAGCCTCCAGC
CCCCCTGGACCTCCTGGGACGACCCCCCCCGGGCAGAAGTCTCCCAGCGGGCCCTTCTGC
ACTCCCCAGTCCCCCGTCTCCCTCGCGCAGGAGGGCACCGCCCAGATCTGGAAGAGGCAG
TCTCAGGAAGTGGAGGCCGGGGGCATCCAGACGGTGGAGGATGAGCACGTGGCCGAGCTG
ACCCTGATGGGGGAGGGGGACATCAGCAGCCCCAACAGCACCTTGCTGCCGCCGCCCTCC
ACCCCCCAGGAAGTGCCAGGACCTTCCAGACCAGGAAGTGGGACCCATGGCAGCCGAGAC
CTCCCAGTCAACAGAATCATCGATGTGGATCTTGTAACTGGCTCGGCGCCCGGGCGGGAT
GGTGGCATAGCGGGGGCACAGGCTGGCCCTGGGAGGTCGCAGGCCAGTGGTGGGCCTCCT
ACATCAGACCTCCCTGGCTTGGGCCCCGTCCCGGGTGAGCCTGCTAAGCCCAAGACCAGC
GCTCACCACGCCACCTTCGTC

Protein sequence predicted based on nucleotide sequence

MFRAGEASKRPLPGPSPPRVRSVEVARGRAGYGFTLSGQAPCVLSCVMRGSPADFVGLRA
GDQILAVNEINVKKASHEDVVKLIGKCSGVLHMVIAEGVGRFESCSSDEEGGLYEGKGWL
KPKLDSKALGINRAERVVEEMQSGGIFNMIFENPSLCASNSEPLKLKQRSLSESAATRFD
VGHESINNPNPNMLSKEEISKVIHDDSVFSIGLESHDDFALDASILNVAMIVGYLGSIEL
PSTSSNLESDSLQAIRGCMRRLRAEQKIHSLVTMKIMHDCVQLSTDKAGVVAEYPAEKLA
FSAVCPDDRRFFGLVTMQTNDDGSLAQEEEGALRTSCHVFMVDPDLFNHKIHQGIARRFG
FECTADPDTNGCLEFPASSLPVLQFISVLYRDMGELIEGMRARAFLDGDADAHQNNSTSS
NSDSGIGNFHQEEKSNRVLVVDLGGSSSRHGPGGSAWDGVGGRGAQPWGAPWTGPFCPDP
EGSPPFEAAHQTDRFWDLNKHLGPASPVEVPPASLRSSVPPSKRGTVGAGCGFNQRWLPV
HVLREWQCGHTSDQDSYTDSTDGWSSINCGTLPPPMSKIPADRYRVEGSFAQPPLNAPKR
EWSRKAFGMQSIFGPHRNVRKTKEDKKGSKFGRGTGLTQPSQRTSARRSFGRSKRFSITR
SLDDLESATVSDGELTGADLKDCVSNNSLSSNASLPSVQSCRRLRERRVASWAVSFERLL
QDPVGVRYFSDFLRKEFSEENILFWQACEYFNHVPAHDKKELSYRAREIFSKFLCSKATT
PVNIDSQAQLADDVLRAPHPDMFKEQQLQIFNLMKFDSYTRFLKSPLYQECILAEVEGRA
LPDSQQVPSSPASKHSLGSDHSSVSTPKKLSGKSKSGRSLNEELGDEDSEKKRKGAFFSW
SRTRSTGRSQKKREHGDHADDALHANGGLCRRESQGSVSSAGSLDLSEACRTLAPEKDKA
TKHCCIHLPDGTSCVVAVKAGFSIKDILSGLCERHGINGAAADLFLVGGDKPLVLHQDSS
ILESRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVARYGLDLSGLLVRLSG
EKEPLDLGAPISSLDGQRVVLEEKDPSRGKASADKQKGVPVKQNTAVNSSSRNHSATGEE
RTLGKSNSIKIKGENGKNARDPRLSKREESIAKIGKKKYQKINLDEAEEFFELISKAQSN
RADDQRGLLRKEDLVLPEFLRLPPGSTELTLPTPAAVAKGFSKRSATGNGRESASQPGEQ
WEPVQESSDSPSTSPGSASSPPGPPGTTPPGQKSPSGPFCTPQSPVSLAQEGTAQIWKRQ
SQEVEAGGIQTVEDEHVAELTLMGEGDISSPNSTLLPPPSTPQEVPGPSRPGSGTHGSRD
LPVNRIIDVDLVTGSAPGRDGGIAGAQAGPGRSQASGGPPTSDLPGLGPVPGEPAKPKTS
AHHATFV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 5248
Start on reference sequence 905
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable