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Nlrc3 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Nlrc3
Gene Name:  NLR family, CARD domain containing 3
Sequence             
Original Clone ID: FLH481516.01X
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00295261

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 3309nts         Open reading frame : 35 to 3309

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCAGGCGGAGCCCTTTAGCACCCT
GGAGCAGCCACCATGGCAGGAAGGAGACAACATAGGTTCCCCAGGGTCAGTCCTGGCTCT
GTATTCACAACTTCTGGCTGCAAACACAGACTCCACGAGGAAGCAAGAGGTGTGGACAGA
CAGAGAGACATGCCTGGCCTACAGTGTTGGCTCCCCAGCTGAGCAGGTGAAAGCCCTTGT
GGATCTGCTGGCTGGGAAGGGCAGTCAGCTGCTACAAGTCCGGGACAAAATGCCAGACTC
CCCACTAGGATCCCAGAGCAATGAGTCAAGGATACCGAAGCACTCTGAGGCTCTGCTGAG
CAGGGTGGGAAATGACCCAGAACTGGGCAGCCCCTCACACCGGCTGGCCAGCCTCATGCT
GGTCGAGGGCCTGACAGACCTGCAGCTAAAGGAGCATGACTTCACACAGGTGGAGGCCAC
GCGTGGGGTCTGGCACCCTGCCAGAGTTATCACCCTGGACAGGCTCTTCCTGCCTCTGTC
CCGGGTATCCATCCCACCTCGAGTCTCTCTCACCATTGGAGTGGCTGGTGTGGGCAAGAC
CACGCTAGTGAGGCATTTTGTTCATTGCTGGGCCAGAGGACAGGTGGGCAAGGGCTTCTC
ACGGGTTCTGCCCTTGACCTTTCGGGATCTCAACACCTATGAGAAACTGTCTGCAGACAG
ACTCATCCAATCCATCTTCTCAAGCATTGGGGAAGCTAGTCTGGTGGCCACAGCCCCAGA
CAGAGTCCTCCTGGTCCTGGATGGCTTGGATGAGTGTAAGACACCCCTGGAATTCTCCAA
TACCATGGCCTGCTCAGACCCAAAGAAGGAGATCCAGGTAGACCACCTGATCACTAACAT
CATCCGAGGCAACCTCTTTCCAGAAATTTCTGTCTGGATCACCTCCCGGCCCAGTGCTGC
TGGTCAGATCCCTGGGGGCCTAGTGGACCGGATGACTGAGATTCGGGGCCTTACTGAGGA
AGAGATCAAAGTGTGTCTGGAGCAGATGTTTCCTGAGGAGCAGAACCTCTTAGGTCAGGT
CCTTAGTCAAGTGCAGGCCAACAGGGCTCTGTATCTGATGTGCACTGTACCAGCCTTTTG
TAGGCTCACGGGGCTGGCTCTGGGTCACTTGTATCGCACCAGGCTGGCCGTCCAAGACAT
AGAGCTGCCATTGCCTCAGACCCTGTGTGAGCTCTACTCTTGGTACTTTAGGATGGCTCT
TGGTGGGGAGGGCCAGGATAAGGAAAAGGTAAGTCCTAGGATCAAGCAGGTGACCCAGGG
AGCTCGCAAAATGGTGGGGACATTGGGCCGCCTGGCCTTCCATGGGCTGGTCAAGAAGAA
ATACGTGTTTTATGAACAAGACATGAAGGCATTTGGAGTGGACCTCGCTCTGTTGCAGAA
CACTCTGTGCAGCTGTCTCCTGCAGCGGGAAGAGACCCTGGCCTCCTCTGTAGCTTACTG
CTTCATTCACCTGTCTCTGCAAGAATTTGTGGCAGCTACATATTACTATAGTGCATCCAA
GAGGGCCATCTTTGACCTCTTCACCGAGAGTGGCATGTCCTGGCCCAGACTGGGTTTCCT
CGCCCATTTCAGGTGTGCAGCCCAGCGGGCCACACAAGCTAAGGATGGAAGGCTGGATGT
GTTTCTGCGCTTCCTCTCTGGCCTCTTGTCCCCAAGGGTCAATACTCTGCTGGCCGGCTC
CCTGTTGTCCCAAGGCGAGCATCAGAGCTACCGGGACCAGGTGGCTGAGGTCCTACAAGG
CTTCCTTCATCCTGACGCTGCAGTCTGTGCACGTGCCATCAATGTCTTGTACTGCCTAAG
TGAGCTGCGGCACACAGAACTGGCCTGCAGTGTGGAGGAGGCCATGCGGAGTGGGACCTT
GGCTGGGATGACCAGCCCCTCACACCGCACTGCTCTGGCCTACCTCCTGCAGATGTCTGA
CATCTGCTCCCCAGAGGCTGACTTCTCCCTGTGTCTCAGCCAGCATGTCCTCCAGAGCCT
GCTGCCCCAGCTGCTCTATTGTCAAAGCCTCAGGCTGGACAACAACCAGTTCCAGGACCC
TGTGATGGAGTTGCTGGGCAGCGTGCTGAGTGGGAAGGACTGTCGCATTCGAAAGATCAG
CCTGGCTGAGAATCAGATTGGTAACAAAGGAGCCAAAGCCCTGGCCAGATCCCTCCTGGT
TAACAGAAGCCTCATCACACTGGACCTCCGGAGTAACAGCATTGGACCACCGGGGGCTAA
GGCTTTGGCCGATGCTCTGAAGATAAACCGAACGCTAACTTCTCTAAGCCTCCAAAGCAA
CGTGATCAAGGATGACGGTGTCATGTGCGTGGCTGAGGCCCTGGTCTCCAACCAGACCAT
CTCCATGCTACAGCTACAGAAGAACTTAATTGGGCTCATAGGAGCCCAGCAGATGGCAGA
TGCCCTGAAGCAGAACAGGAGCCTGAAAGCACTCATGTTTTCCAGTAATACCATTGGCGA
CAGAGGTGCCATAGCCCTGGCTGAGGCCCTGAAGGTGAACCAGATCCTGGAGAACTTAGA
CCTACAGAGCAATTCCATCAGTGACATGGGAGTGACGGTGCTGATGCGAGCCCTCTGCAG
TAACCAGACACTCTCCAGTCTCAACCTGCGAGAAAACTCCATCAGCCCAGAGGGAGCCCA
GGCCCTCACTCAAGCTCTCTGCAGGAACAACACTCTGAAGCACTTGGACCTGACAGCTAA
TCTCCTCCATGACCGAGGTGCCCAGGCCATTGCAGTAGCTGTGGGAGAAAACCACTCCCT
CACACACCTTCACCTGCAGTGGAACTTCATCCAAGCTGGTGCGGCCAGGGCCCTGGGACA
AGCACTCCAGCTGAACAGAACCCTGACAACCTTAGACTTACAGGAGAATGCCATAGGGGA
TGAAGGAGCTTCCTCAGTGGCTGGCGCACTGAAGGTGAACACAACCCTCATTGCTCTCTA
CCTACAGGTGGCCTCCATTGGTAGCCAAGGGGCCCAGGCACTTGGGGAGGCCCTCACTGT
GAACAGAACCTTGGAGATTCTTGACTTACGAGGAAACGACGTTGGGGCAGCTGGAGCCAA
GGCCTTGGCAAATGCTTTAAAGTTAAACTCCAGTCTCCGAAGACTCAATCTCCAGGAGAA
CTCACTGGGGATGGATGGGGCCATATTTGTTGCCTCTGCACTGTCTGAGAACCACGGTCT
GCACCATATTAATCTCCAGGGGAATCCCATTGGGGAGTCTGCTGCCAGGATGATCTCAGA
GGCCATCAAGACAAACGCTCCCACATGCACTGTGGAAATATAGGGCCTCATGGGCCCAGC
TTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3564
Start on reference sequence 256
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Nlrc3
Symbol Nomenclature : Nlrc3
SYNONYM : CLR16.2; D230007K08Rik; mFLJ00348
Designation : Caterpiller 16.2|caterpillar 16.2|protein NLRC3
Full Nomenclature : NLR family, CARD domain containing 3
GENEID : 268857
GI : 182887926
GenBank Accession : BC160196
Mouse Genome Informatics : 2444070
Target GenBank: NM_001081280.1

 

Clone

Cloning Information : 481516
HIP Master Clone ID : 399244
IMAGE ID : 100063844
Original Clone ID : FLH481516.01X

Organism


TAX_ID : 10090
Species Specific ID: 268857

Genome

Chromosome : 16
Map Location : 16 A1|16

Reagents

Labome : Nlrc3-antibody