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Lgals6 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Lgals6
Gene Name:  lectin, galactose binding, soluble 6
Sequence             
Original Clone ID: FLH481595.01X
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00295340

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 906nts         Open reading frame : 35 to 906

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGCCTATGTTCCAGCACCGGGCTA
CCAGCCCACCTACAATCCGACTCTGCCCTACAAGAGACCCATCCCAGGTGGCCTCAGTGT
CGGGATGTCCTTTTACATCCAAGGAACGGCCAAAGAGAACATGAGACGGTTCCACGTGAA
CTTCGCTGTGGGGCAGGATGATGGGGCGGATGTTGCTTTCCACTTCAATCCCCGCTTTGA
TGGCTGGGACAAGGTGGTCTTCAACACGAAGCAAAGCGGACGATGGGGCAAGGAAGAGGA
GAAGAGCATGCCTTTCCAGAAGGGCAAGCACTTCGAGCTGGTGTTCATGGTCATGCCTGA
GCACTACAAGGTCGTGGTGAATGGAAGTCCCTTCTATGAATACGGGCACCGGTTACCCGT
ACAGATGGTCACCCATCTGCAAGTGGATGGGGACCTGGAACTTCAGTCCATCAACTTCTT
TGGAGTCCAACCTGCTGAAACCAAATATCCGGCCATGACAGGACCCCCAGTCTTCAATCC
GTGTCTGCCATATGTGGGGGCTCTGCAGGGGGGCTTTACAGTCCGAAGAACCATCATAAT
CAAGGGATATGTACTTCCTACAGCCAAGACCTTTGCCATCAACTTCAGGGTGGGATCTTC
CGAAGACATAGCTTTACACATAAACCCCCGCATAGGTGACTGTTTGGTTCGCAACAGCTA
TATGAATGGCTCTTGGGGCACTGAAGAGAGGATGGTGGCCTACAACCCATTTGGCCCTGG
GCAGTTCTTTGATCTGTCAATCCGCTGCGGCATGGATCGATTCAAGGTGTTTGCCAATGG
CATTCACCTTTTCAACTTCTCCCATCGGTTCCAAGCTTTACGAAAGATAAACACGCTGGA
GATCAATGGTGACCTCACCTTGTCCTACGTCCACATCTAGGGCCTCATGGGCCCAGCTTT
CTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 958
Start on reference sequence 53
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Lgals6
Symbol Nomenclature : Lgals6
SYNONYM : galectin-6
Designation : gal-6|galectin-6
Full Nomenclature : lectin, galactose binding, soluble 6
GENEID : 16857
GI : 182887994
GenBank Accession : BC160275
Mouse Genome Informatics : 107535
Target GenBank: NM_010707.1

 

Clone

Cloning Information : 481595
HIP Master Clone ID : 399323
IMAGE ID : 100063923
Original Clone ID : FLH481595.01X

Organism


TAX_ID : 10090
Species Specific ID: 16857

Genome

Chromosome : 7
Map Location : 7 B1|7

Reagents

Labome : galectin-6-siRNA