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Myocd (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Myocd
Gene Name:  myocardin
Sequence             
Original Clone ID: FLH481643.01X
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00295396

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2952nts         Open reading frame : 35 to 2952

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGACACTCCTGGGGTCTGAACACTC
TTTGCTGATTAGAAGGAAGTTCCGATCAGTCTTACAGTTACGGCTTCAACAGAGAAGGAC
CCAGGAGCAGCTGGCTAACCAAGGCTTAATACCGCCACTGAAAGGTCCAACTGAATTCCA
TGACCCGAGAAAACAATTGGATAGTGCCAAGACTGAAGATTCCCTGAGGCGCAAGGGCAG
AAACAGGTCCGACCGTGCCAGCCTGGTTACTATGCACATTCTCCAAGCCTCCACGGCAGA
AAGGTCCATTCCAACTGCTCAGATGAAGCTCAAAAGAGCCCGCCTTGCAGATGACCTCAA
TGAGAAGATCGCTCTCCGCCCAGGGCCCTTGGAACTGGTGGAGAAGAACATTCTGCCGAT
GGATTCTTCCGTGAAAGAGGCTATAAAAGGTACTGAGGTGAGCCTCTCCAAGGCAGCAGA
TGCATTCGCCTTTGAGGATGACAGCAGTAGAGATGGGCTCTCTCCAGATCAGGCTAGGAG
CGAGGACCCCCAGGGCTCTACAGGATCCACCCCAGACATCAAATCCACTGAGGCTCCTCT
GGACACAATCCAGGATCTCACTCCTGGCTCAGAAAGTGACAAGAATGATGCAGCCTCCCA
GCCAGGCAACCAGTCAGACCCTGGGAAGCAGGTTCTCGGCCCCCTCAGCACCCCGATTCC
TGTGCACACTGCTGTAAAGTCCAAGTCTTTGGGTGACAGTAAGAACCGCCACAAAAAGCC
CAAAGACCCCAAACCAAAGGTGAAGAAGCTCAAATACCATCAGTACATCCCCCCAGACCA
GAAGGCAGAGAAGTCTCCCCCACCCATGGACTCTGCCTATGCCCGGCTGCTCCAGCAACA
GCAGCTATTCCTGCAGCTACAGATCCTCAGCCAGCAGCAGCAACAGCAGCAGCAACAGCA
GCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCGGTTCAGCTACCCTGGGATGCACCA
AACACACCTCAAAGAACCAAATGAACAGATGGCCAGAAATCCGAATCCTTCTTCAACACC
ACTGAGCAATACCCCTCTATCCCCTGTCAAAAATAGCATTTCTGGACAAACTGGTGTTTC
TTCTCTCAAACCAGGCCCCCTCCCACCCAACCTGGATGATCTCAAGGTGTCAGAGTTAAG
ACAACAGCTTCGAATCCGGGGCTTGCCAGTGTCAGGCACCAAGACAGCGCTGGTGGACCG
GCTTCGTCCCTTCCAGGATTGTGCTGGCAACCCTGTGCCCAACTTTGGGGACATCACAAC
TGTCACCTTTCCTGTCACGCCCAACACCTTGCCCAGTTATCAGTCCTCCCCGACAGGCTT
CTACCACTTTGGCAGCACAAGCTCCAGCCCACCCATCTCCCCCGCCTCATCTGACTTGTC
CGCTGCAGGGTCCCTGCCAGACACCTTCACCGATGCGTCACCTGGCTTCGGCCTGCACGC
ATCTCCGGTGCCCGCCTGCACGGACGAGAGTCTGCTGAGCAGCCTGAATGGGGGCTCGGG
CCCCTCCGAGCCTGATGGGCTAGACTCTGAGAAGGACAAGATGCTGGTGGAGAAGCAGAA
AGTGATCAACCAGCTCACCTGGAAGCTGCGGCAAGAGCAGCGGCAGGTGGAAGAGCTGAG
AATGCAACTGCAGAAGCAGAAGAGCAGCTGCAGCGACCAGAAGCCACTGCCCTTCTTGGC
CACCACCATCAAACAGGAAGATGTCTCCAGCTGCCCCTTCGCACCCCAGCAGGCGTCTGG
GAAGGGACAGGGCCACAGCTCTGACAGTCCCCCTCCGGCTTGTGAGACGGCTCAGCTGCT
GCCTCACTGTGTGGAGTCCTCAGGTCAAACCCATGTACTCTCGTCCACGTTTCTCAGCCC
CCAGTGCTCCCCTCAGCACTCGCCCCTGGGGGGCCTGAAGAGCCCGCAGCACATCAGCCT
GCCTCCATCACCCAACAACCATTACTTCCTGGCTTCCTCTTCGGGAGCTCAGAGAGAGAA
CCATGGGGTCTCTTCACCCAGCAGCAGCCAAGGGTGCGCACAGAACTCAGGGGCACACGA
AGGCCATTCTTCTAGCTTCTCTTCCCCAGCTTCCAGCCTCCATCAGCCTTTCTCTGGCAC
CCAAGCAGACAGCAGTCACAGTGCTGGGCTCAACCCTTGTCCCAAAAGCCCAAGTATTCA
TCCAAAGATGACTGGTTTACAATCTTCTGACAAGGTGGGGCCAACGTTTTCAATTCCATC
CCCAACTTTTTCTAAGTCAAGTTCAGCAGTTTCAGATATCACCCAGCCCCCATCCTATGA
AGATGCAGTGAAGCAGCAAATGACTCGGAGTCAGCAGATGGACGAACTCCTGGATGTCCT
CATTGAAAGTGGAGAAATGCCAGCCGATGCCAGGGAAGATCATTCATGTCTTCAGAAAAT
TCCAAAGATCCCTGGGTCCTCCTGCAGCCCAACTGCCATCCCCCCGAAGCCCTCGGCTTC
CTTTGAGCAGGCATCTTCGGGAGGCCAGATGGCCTTCGATCACTACGCCAACGACAGTGA
CGAACACCTGGAAGTCTTATTGAATTCTCACAGCCCCATCGGAAAGGTGAGCGATGTTAC
CCTCCTCAAAATCGGAAGCGAGGAGCCTCCTTTTGACAGCATCATGGATGGCTTCCCAGG
GAAGGCTGCGGAAGATCTCTTCAGTGCTCACGAGCTCTTGCCTGGGCCCCTCTCCCCGAT
GCATGCACAGTTGTCACCTCCTTCTGTGGACAGCAGTGGTCTGCAGCTGAGCTTCACGGA
ATCTCCTTGGGAAACAATGGAATGGCTGGACCTCACTCCACCTAGTTCCACGCCAGGCTT
CAGCAACCTTACCTCCAGTGGGCCCAGCATTTTCAACATCGATTTTCTGGATGTTACAGA
TCTTAATCTGAATTCCCCTATGGATCTCCACTTACAGCAGTGGTAGGGCCTCATGGGCCC
AGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3243
Start on reference sequence 292
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Myocd
Symbol Nomenclature : Myocd
SYNONYM : BSAC2A; Srfcp
Designation : SRF co-factor protein (cardiac and smooth muscle)|SRF cofactor protein|basic SAP coiled-coil transcription activator 2
Full Nomenclature : myocardin
GENEID : 214384
Locus Tag : RP23-298L23.1
GI : 189442142
GenBank Accession : BC167199
Mouse Genome Informatics : 2137495
Vega : OTTMUSG00000005903
Target GenBank: NM_145136.4

 

Clone

Cloning Information : 481643
HIP Master Clone ID : 399371
IMAGE ID : 100068099
Original Clone ID : FLH481643.01X

Organism


TAX_ID : 10090
Species Specific ID: 214384

Genome

Chromosome : 11
Map Location : 11 B3|11
Ensembl : ENSMUSG00000020542

Reagents

Labome : Myocd-antibody