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Nisch (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Nisch
Gene Name:  nischarin
Sequence             
Original Clone ID: FLH481700.01X
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00295453

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 4782nts         Open reading frame : 35 to 4816

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGCGGCTGCGACACTCAGCTTCGG
CCCTGAGCGGGAGGCCGAGCCTGCCAAGGAGGCGCGCGTCGTGGGTTCTGAGCTCGTGGA
CACGTACACGGTTTATGTCATCCAGGTTACCGATGGCAACCATGAGTGGACGATCAAACA
CCGTTACAGTGATTTTCATGACCTACATGAAAAGCTTGTGGCAGAGAGAAAAATTGACAA
ATCTCTACTTCCACCCAAAAAGATAATTGGGAAGAACTCGAGAAGCTTGGTAGAGAAGAG
GGAGAGAGACCTGGAGGTGTACCTCCAGACGCTCCTCACAACCTTCCCTGATGTGGCTCC
CAGAGTTCTGGCCCACTTCCTGCATTTTCACCTCTATGAAGTAAATGGTGTCACTGCAGC
ACTGGCTGAAGAACTCTTTGAAAAAGGAGAGCAACTTCTAGGAGCCGGTGAGGTCTTTGC
CATCAGGCCCTTGCAGCTCTATGCTATCACAGAACAGCTGCAACAGGGAAAGCCTACTTG
TGCCAGTGGTGATGCCAAGACTGACCTTGGACACATCCTAGACTTCACCTGTCGCCTTAA
GTATCTTAAGGTTTCAGGCACAGAAGGACCTTTTGGGACCAGCAATATTAAGGAGCAGCT
CTTGCCCTTTGATCTTTCAATATTCAAGTCTCTTCACCAGGTGGAGATAAGTCATTGTGA
TGCCAAGCATATCCGAGGGCTGGTCACCTCCAAGCCCACTTTAGCCACAATGAGTGTTAG
ATTCTCAGCAACCTCAATGAAGGAAGTGCTTGCTCCTGAAGCCTCAGAATTTGATGAGTG
GGAGCCTGAAGGCACTGCTACCCTCGGAGGCCCTGTGACTGCTATCATCCCAACATGGCA
AGCACTGACTACTCTAGACCTGAGCCACAACAGCATCTGTGAGATCGACGAATCTGTGAA
ACTGATCCCAAAGATAGAGTACCTGGACCTGAGTCACAATGGACTGCGAGTTGTGGATAA
CCTGCAGCACCTATACAACCTCGTGCACCTTGACCTGTCCTACAACAAGCTCTCCTCCCT
GGAAGGCGTGCACACTAAACTGGGCAATGTCAAAACCCTAAACCTGGCAGGCAACTTCTT
AGAGAGTCTGAGCGGTCTGCACAAACTCTACTCCTTGGTTAATGTGGACCTACGAGACAA
CCGGATTGAGCAGTTGGATGAGGTCAAGAGCATTGGCAGCCTGCCATGTCTGGAGCGTTT
GACCTTGCTGAACAACCCTTTGAGCATCATCCCTGACTACCGGACCAAGGTGCTTTCCCA
GTTTGGAGAACGAGCCTCTGAGATTTGTCTAGATGATGTCGCAACCACAGAGAAAGAGCT
GGACACTGTGGAAGTGCTGAAGGCAATTCAGAAAGCCAAAGATGTCAAGTCCAAACTGAG
CAACACAGAAAAGAAGGCTGGTGAGGACTTCCGGCTCCCGCCTGCACCCTGCATCAGACC
CGGCGGCTCCCCTCCTGCAGCTCCCGCCTCAGCCTCCCTGCCTCAGCCGATCCTCTCCAA
CCAAGGTATCATGTTTGTACAGGAAGAGGCCCTGGCCAGCAGCCTCTCATCCACGGATAG
TCTGCCACCCGAGGACCACCGGCCCATTGCCCGAGCCTGCTCTGACTCCTTGGAATCTAT
CCCTGCAGGACAGGTGGCCTCTGATGATCTGAGGGATGTGCCAGGAGCTGTTGGCGGCGT
GAGCCCAGATCATGCAGAGCCAGAGGTTCAGGTGGTGCCTGGGTCCGGCCAGATCATCTT
CCTGCCCTTCACTTGCATTGGCTACACAGCCACCAACCAGGACTTTATCCAGCGCCTCAG
CACACTGATCCGCCAGGCCATTGAACGGCAACTCCCTGCCTGGATTGAGGCTGCCAACCA
GCGCGAGGAAGCCCATGGTGAGCAGGGCGAGGAGGAAGAAGAGGAAGAGGAAGAGGAGGA
TGTTGCTGAGAACCGCTACTTTGAAATGGGACCCCCAGACGCAGAGGAAGAGGAGGGGAG
TGGCCAGGGAGAAGAGGATGAGGAAGACGAGGACGAGGAGGCGGAGGAGGAGCGCCTGGC
TCTGGAGTGGGCCCTGGGCGCCGATGAGGACTTCCTGCTGGAGCACATCCGCATCCTCAA
GGTGCTCTGGTGCTTCCTGATCCACGTGCAAGGCAGCATCCGCCAGTTCGCTGCCTGCCT
TGTGCTCACTGACTTCGGCATCGCAGTCTTTGAGATCCCACACCAAGAGTCAAGAGGCAG
CAGCCAGCACATCCTCTCATCCCTGCGTTTTGTCTTCTGCTTCCCACATGGCGACCTCAC
GGAGTTTGGCTTCCTCATGCCCGAGCTGTGTCTGGTGCTCAAGGTGCGGCACAGTGAGAA
CACGCTCTTCATCATCTCGGATGCTGCCAACTTACACGAGTTCCATGCTGATCTACGCTC
CTGCTTTGCACCGCAGCACATGGCCATGCTGTGCAGCCCCATCCTCTACGGCAGCCACAC
CACCCTGCAGGAGTTCTTGCGCCAGCTGCTCACCTTCTACAAGGTGGCCGGGGGCTCTCA
GGAGCGCAGCCAGGGCTGCTTCCCTGTCTACCTGGTCTACAGTGACAAGCGCATGGTCCA
GACCCCTGCCGGGGACTATTCAGGCAATATCGAATGGGCCAGCTGCACGCTGTGCTCGGC
GGTGCGGCGCTCCTGCTGCGCGCCCTCGGAGGCCGTCAAGTCCGCTGCCATCCCCTACTG
GCTGCTGCTCACATCCCAGCATCTCAACGTCATCAAGGCCGACTTCAACCCCATGCCCAA
TCGAGGCACCCACAACTGCCGCAACCGCAACAGCTTCAAGCTTAGCCGCGTCCCGCTCTC
TACCGTGCTGCTAGACCCCACTCGCAGCTGCACCCAGCCACGGGGTGCCTTCGCTGATGG
CCATGTGCTCGAGCTGCTCGTGGGCTACCGCTTCGTTACCGCCATCTTTGTGCTGCCCCA
CGAGAAATTCCACTTCCTGCGAGTCTACAATCAGCTACGCGCCTCACTGCAGGACCTGAA
GACTGTGGTCATCTCCAAGAATCCTTCAGCTAAGCCAAGAAATCAGCCTGCCAAGAGCAG
GGCCAGTGCTGAGCAGCGGCTACAGGAGACCCCAGCAGACGCTCCGGCTCCAGCTGCAGT
CCCACCAACAGCTTCAGCTCCAGCCCCCGCAGAGGCCTTGGCTCCAGATCTGGCTCCTGT
ACAGGCCCCAGGAGAGGACCGAGGTCTAACTTCAGCAGAGGCTCCAGCCGCAGCAGAGGC
TCCAGCCGCAGCAGAGGCTCCAGCCGCAGCAGAGGCTCCAGCCGCAGCAGAGGCTCCAGC
CGCAGCAGAGGCTCCAGCCGCAGCAGAGGCTCCGGCCCCAGCAGAGGCTCCAGCCGCAGC
AGAGGCTCCGGCCGCAGCAGAGGCTCCAGCTGCAGCAGAGGCTCCAGCCGCAGCAGAGGC
TCCAGCTTCAGCAGAGGCTCCAGCTCCAAACCAGGCTCCTGCTCCAGCAAGGGGTCCCGC
TCCAGCAAGGGGTCCCGCTCCAGCAGGGGGTCCCGCTCCAGCAGAGGCCCTGGCACAAGC
AGAAGTCCCTGCCCAGTACCCAAGTGAGCGCCTAATCCAGTCCACATCTGAAGAGAATCA
GATCCCTTCTCACTTGCCAGTATGCCCATCACTCCAGCACATTGCCCGTCTTCGGGGGAG
AGCCATCATTGACCTCTTCCACAACAGCATTGCTGAGGTTGAAAATGAGGAGCTGAGGCA
CCTCCTGTGGTCATCAGTGGTGTTCTACCAGACCCCGGGGCTAGAAGTGACCGCCTGTGT
GCTGCTCTCTAGCAAGGCTGTGTACTTCATACTGCATGATGGTCTCCGCCGCTACTTCTC
TGAACCACTGCAGGATTTCTGGCACCAGAAAAACACCGACTATAACAACAGTCCTTTCCA
CGTCTCTCAGTGCTTTGTGTTGAAACTCAGTGACCTGCAGTCAGTCAACGTCGGCCTTTT
CGACCAGTACTTCCGGCTGACGGGCTCCTCCCCGACGCAGGTGGTCACGTGCTTGACTCG
CGACAGCTACCTGACGCACTGCTTCCTCCAGCATCTGATGCTTGTGCTGTCCTCCCTGGA
GCGCACACCCTCGCCTGAGCCTGTTGACAAGGACTTCTACTCAGAATTTGGGGACAAGAA
TACAGGGAAAATGGAGAACTATGAGCTGATCCATTCCAGCCGCGTCAAGTTCACCTACCC
CAGTGAGGAAGAGGTTGGGGACCTGACCTATATTGTCGCACAGAAGATGGCTGATCCTGC
AAAGAATCCAGCCCTCAGCATCTTACTGTACATCCAGGCCTTCCAGGTGGTCACACCACA
CCTTGGGCGGGGCAGGGGCCCACTGCGCCCTAAGACGCTGCTCCTGACCAGCGCCGAGAT
CTTCCTCCTGGATGAGGACTACATCCACTATCCATTGCCTGAATTTGCCAAAGAGCCACC
GCAGAGGGACAGATACCGGCTAGACGATGGCCGCCGGGTCCGGGATTTGGACCGGGTGCT
CATGGGCTACTATCCCTACCCACAGGCCCTCACTCTTGTTTTTGATGACACACAGGGTCA
CGACCTCATGGGGAGTGTCACCCTGGACCACTTTGGGGAGATGCCAGGTGGTCCTGGCAG
GGTTGGCCAGGGCCGAGAGGTACAGTGGCAGGTGTTTGTCCCCAGTGCCGAGAGCCGAGA
AAAGCTCATCTCACTGCTCGCACGACAGTGGGAAGCTCTTTGTGGCAGGGAGCTGCCTGT
GGAGCTCACTGGCTAGGGCCTCATGGGCCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 4946
Start on reference sequence 165
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Nisch
Symbol Nomenclature : Nisch
SYNONYM : 1200007D05Rik; 3202002H23Rik; AW494485; I-1
Designation : I1R|IR1|imidazoline receptor 1|imidazoline receptor I-1-like protein|imidazoline-1 receptor
Full Nomenclature : nischarin
GENEID : 64652
GI : 189442555
GenBank Accession : BC167256
Mouse Genome Informatics : 1928323
Vega : OTTMUSG00000035875
Target GenBank: NM_022656.2

 

Clone

Cloning Information : 481700
HIP Master Clone ID : 399428
IMAGE ID : 100068156
Original Clone ID : FLH481700.01X

Organism


TAX_ID : 10090
Species Specific ID: 64652

Genome

Chromosome : 14
Map Location : 14 B|14
Ensembl : ENSMUSG00000021910

Reagents

Labome : Nisch-antibody