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HNRNPUL1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  HNRNPUL1
Gene Name:  heterogeneous nuclear ribonucleoprotein U-like 1
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH481954.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00295612

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2571nts         Open reading frame : 23 to 2571

GTACAAAAAAGCAGGCTCCACCATGGATGTGCGCCGTCTGAAGGTGAACGAACTTCGCGA
GGAGCTGCAGCGCCGCGGCCTGGACACTCGAGGCCTCAAGGCCGAGCTTGCTGAGCGGCT
GCAGGCGGCGTTGGAGGCCGAGGAGCCTGACGACGAGCGGGAGCTCGACGCCGACGACGA
ACCGGGGCGACCCGGGCACATCAACGAGGAGGTCGAGACCGAGGGGGGCTCCGAGCTGGA
GGGGACCGCGCAGCCACCGCCGCCCGGGCTGCAGCCGCACGCGGAGCCCGGCTGCTACTC
GGGGCCGGACGGACATTATGCCATGGACAATATTACCAGGCAGAACCAATTCTACGATAC
CCAAGTCATCAAACAAGAAAACGAGTCAGGCTACGAGAGGAGACCACTGGAAATGGAGCA
GCAGCAGGCCTATCGTCCAGAAATGAAGACAGAGATGAAGCAAGGAGCACCCACCAGCTT
CCTCCCGCCTGAAGCTTCTCAACTCAAGCCAGACAGGCAGCAATTCCAGAGTCGAAAGAG
GCCTTATGAAGAAAACCGGGGACGGGGGTACTTTGAGCACCGAGAGGATAGGAGGGGCCG
CTCTCCTCAGCCTCCTGCTGAAGAGGATGAAGATGACTTTGATGATACCCTTGTTGCTAT
TGACACCTATAACTGCGACCTCCACTTCAAGGTGGCCCGAGATCGGAGTAGTGGCTATCC
GCTCACAATTGAGGGCTTTGCATACCTGTGGTCAGGAGCCCGTGCCAGCTATGGGGTCAG
AAGGGGCCGTGTATGCTTCGAGATGAAGATCAATGAGGAAATCTCCGTGAAGCACCTTCC
GTCTACAGAGCCTGACCCCCACGTGGTCCGTATCGGCTGGTCCCTGGACTCCTGCAGCAC
CCAGCTAGGCGAAGAGCCTTTCTCCTATGGCTATGGAGGCACTGGGAAGAAGTCCACCAA
TAGCCGGTTTGAAAACTACGGAGACAAGTTTGCAGAGAACGATGTGATTGGCTGCTTTGC
GGATTTTGAATGTGGAAATGACGTGGAACTGTCTTTTACCAAGAATGGAAAGTGGATGGG
CATTGCTTTCCGAATCCAGAAGGAAGCCTTGGGGGGTCAGGCCCTCTATCCTCATGTCCT
GGTGAAGAATTGCGCAGTGGAGTTCAACTTCGGACAGAGAGCAGAGCCCTACTGTTCTGT
CCTCCCGGGGTTTACCTTCATCCAGCACCTTCCCCTTAGTGAGCGTATCCGGGGCACCGT
TGGACCAAAGAGCAAGGCAGAATGTGAGATTCTGATGATGGTGGGCCTGCCTGCTGCTGG
CAAGACCACATGGGCCATCAAACATGCAGCCTCCAACCCTTCCAAGAAGTACAACATCCT
GGGTACCAATGCCATCATGGATAAGATGCGGGTGATGGGCCTACGCCGGCAGCGGAACTA
TGCTGGCCGCTGGGATGTCCTGATCCAGCAGGCCACCCAGTGCCTCAACCGCCTCATCCA
GATTGCTGCCCGCAAGAAACGCAACTATATCCTAGATCAGACAAATGTTTATGGGTCAGC
CCAGAGACGAAAAATGAGACCATTTGAAGGCTTCCAGCGCAAAGCTATTGTAATTTGTCC
CACTGACGAGGACCTAAAAGACCGAACAATAAAGCGAACCGACGAGGAAGGGAAGGATGT
CCCAGATCATGCGGTCTTAGAAATGAAAGCCAACTTCACGTTGCCAGATGTTGGGGACTT
CCTGGATGAGGTTCTGTTCATTGAGCTGCAGCGGGAGGAAGCGGACAAGCTAGTGAGGCA
GTACAACGAGGAAGGCCGCAAGGCTGGGCCACCCCCTGAAAAGCGCTTTGACAACCGAGG
TGGTGGTGGCTTCCGGGGCCGCGGGGGTGGTGGTGGCTTCCAGCGCTATGAAAACCGAGG
ACCCCCTGGAGGCAACCGTGGCGGCTTCCAGAACCGAGGGGGAGGCAGCGGTGGAGGAGG
CAACTACCGAGGAGGTTTCAACCGCAGCGGAGGTGGTGGCTATAGCCAGAACCGCTGGGG
TAACAACAACCGGGATAACAACAACTCCAACAACAGAGGCAGCTACAACCGGGCTCCCCA
GCAACAGCCGCCACCACAGCAGCCTCCGCCACCACAGCCACCACCCCAGCAGCCACCGCC
ACCACCCAGCTACAGCCCTGCTCGGAACCCCCCAGGGGCCAGCACCTACAATAAGAACAG
CAACATCCCTGGCTCAAGCGCCAATACCAGCACCCCCACCGTCAGCAGCTACAGCCCTCC
ACAGCCGAGTTACAGCCAGCCACCCTACAACCAGGGAGGTTACAGCCAGGGCTACACAGC
CCCACCGCCTCCACCTCCACCACCACCTGCCTACAACTATGGGAGCTACGGCGGTTACAA
CCCGGCCCCCTATACCCCACCGCCACCCCCCACCGCACAGACCTACCCTCAGCCCAGCTA
TAACCAGTATCAGCAGTATGCCCAGCAGTGGAACCAGTACTATCAGAACCAGGGCCAGTG
GCCGCCATACTACGGGAACTACGACTACGGGAGCTACTCCGGGAACACACAGGGTGGCAC
AAGTACACAGTGAATCCACCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2641
Start on reference sequence 71
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcaggctCCACC

3' Linker Sequence:
TGGATCCacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HNRNPUL1
Symbol Nomenclature : HNRNPUL1
SYNONYM : E1B-AP5; E1BAP5; HNRPUL1
Designation : E1B 55kDa associated protein 5|E1B-55 kDa-associated protein 5|E1B-55kDa-associated protein 5|adenovirus early region 1B-associated protein 5|heterogeneous nuclear ribonucleoprotein U-like protein 1
Full Nomenclature : heterogeneous nuclear ribonucleoprotein U-like 1
GENEID : 11100
GI : 190690798
GenBank Accession : EU831487
HGNC : 17011
MIM : 605800
Vega : OTTHUMG00000182740
Target GenBank: BC002564.2

 

Pathways

Reactome : Gene Expression
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway

Clone

Cloning Information : 481954
HIP Master Clone ID : 399682
IMAGE ID : 100066516
Original Clone ID : FLH481954.01X

Organism


TAX_ID : 9606
Species Specific ID: 11100

Genome

Chromosome : 19
Map Location : 19q13.2
Ensembl : ENSG00000105323

Reagents

Labome : HNRNPUL1-antibody