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FNDC8 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FNDC8
Gene Name:  fibronectin type III domain containing 8
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH482312.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
HsCD00295970

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 975nts         Open reading frame : 23 to 997
Reference Sequence Alignment

GTACAAAAAAGCAGGCTCCACCATGGCATCAGAGGCACTCCATCAAGTGGGAGATGGGGA
GGAGGCTGTACTGAAGAAAGAAAACTTCAACATGATGAATGCCCTTGACCAACTGCCAAA
ACCCTTTTCAAACCCCAAGTCTATGAACCGGACCGTCACTACCAAAGGACTCCCACTAGC
CTCAAAGGGCAATTTGGTCAACTTCTTGGAGGATGATACCATCAACCTACTGAAGCCGCT
GCCAGTAGAGGATTCAGACTGCAGCTCTGATGAGACCAGCATCTCTGCCTTCTCATCCAC
CTTGCTGAACCCCATCAAATTAGCTGTGACCCAGCCCAACAGCAGCTTCTTTGCAGGGAT
GCTGGAGGGGGAGCTGAACAAACTCAGCTTCTCCCCAATGGCCAAGAATGCAGAAAATGA
GGACCTGGCGCTCGGCCCCTGCCCATGCCCATCGAAGTCCCAGATGGCCACAAGGGGCCT
GCTGGACCTTGACAACCCTGAGCTGGAGACAGAAACCTCCTCAACGCACTCAGAATCTTC
TGTGGTCGTGGACCTGCCGGACACCCCCTTCATCTTTGAGCACACCGTCAACAATTCCAC
AGCTGTGATTTCCTGGACCTACGCCTTGGGCAAGCAGCCGGTCAGTTTCTACCAGCTCCT
GTTACAGGAGGTGGCCAAGACACAGGAGAATGAGTTGCCCGAGGCAAAGAATCGTCCATG
GATCTTCAACAAGATTTTGGGCACTACTGTCAAGCTGATGGAGCTAAAGCCTAACACGTG
TTACTGCCTCAGTGTCCGTGCAGCCAACACAGCTGGGGTGGGGAAGTGGTGCAAGCCCTA
CAAATTTGCAACCCTGGCCACTGACTTCAGCAGCTTTCCTGAGAACTACCCCATCCAGAT
CACCGTGCGGCGCAAGGAACCCCGGCAAAAGATCGTGTCCATCGGGCCGGAGGAGATGCG
GAGGCTGGAGGATCTGGAATACCTATTTCCCTGTTAGCTTGACCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1008
Start on reference sequence 34
Mutations:
None
Discrepancy:
t525c
                                

5' Linker Sequence:
gtacaaaaaagcaggctCCACC

3' Linker Sequence:
TAGCTTGacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site Att P2 Primer 3026 3043
primer site U19 primer 517 535
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
selectable marker KanR kanamycin resistance gene 3156 3965
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FNDC8
Symbol Nomenclature : FNDC8
Designation : fibronectin type III domain-containing protein 8
Full Nomenclature : fibronectin type III domain containing 8
GENEID : 54752
GI : 190690850
GenBank Accession : EU831600
HGNC : 25286
Vega : OTTHUMG00000132932
Target GenBank: AL133054.1

 

Reference Sequence Alignment

  

HsCD00295970      GTACAAAAAAGCAGGCTCCACCATGGCATCAGAGGCACTCCATCAAGTGGGAGATGGGGA
NM_017559.2       ----------------------ATGGCATCAGAGGCACTCCATCAAGTGGGAGATGGGGA
                                        **************************************

HsCD00295970      GGAGGCTGTACTGAAGAAAGAAAACTTCAACATGATGAATGCCCTTGACCAACTGCCAAA
NM_017559.2       GGAGGCTGTACTGAAGAAAGAAAACTTCAACATGATGAATGCCCTTGACCAACTGCCAAA
                  ************************************************************

HsCD00295970      ACCCTTTTCAAACCCCAAGTCTATGAACCGGACCGTCACTACCAAAGGACTCCCACTAGC
NM_017559.2       ACCCTTTTCAAACCCCAAGTCTATGAACCGGACCGTCACTACCAAAGGACTCCCACTAGC
                  ************************************************************

HsCD00295970      CTCAAAGGGCAATTTGGTCAACTTCTTGGAGGATGATACCATCAACCTACTGAAGCCGCT
NM_017559.2       CTCAAAGGGCAATTTGGTCAACTTCTTGGAGGATGATACCATCAACCTACTGAAGCCGCT
                  ************************************************************

HsCD00295970      GCCAGTAGAGGATTCAGACTGCAGCTCTGATGAGACCAGCATCTCTGCCTTCTCATCCAC
NM_017559.2       GCCAGTAGAGGATTCAGACTGCAGCTCTGATGAGACCAGCATCTCTGCCTTCTCATCCAC
                  ************************************************************

HsCD00295970      CTTGCTGAACCCCATCAAATTAGCTGTGACCCAGCCCAACAGCAGCTTCTTTGCAGGGAT
NM_017559.2       CTTGCTGAACCCCATCAAATTAGCTGTGACCCAGCCCAACAGCAGCTTCTTTGCAGGGAT
                  ************************************************************

HsCD00295970      GCTGGAGGGGGAGCTGAACAAACTCAGCTTCTCCCCAATGGCCAAGAATGCAGAAAATGA
NM_017559.2       GCTGGAGGGGGAGCTGAACAAACTCAGCTTCTCCCCAATGGCCAAGAATGCAGAAAATGA
                  ************************************************************

HsCD00295970      GGACCTGGCGCTCGGCCCCTGCCCATGCCCATCGAAGTCCCAGATGGCCACAAGGGGCCT
NM_017559.2       GGACCTGGCGCTCGGCCCCTGCCCATGCCCATCGAAGTCCCAGATGGCCACAAGGGGCCT
                  ************************************************************

HsCD00295970      GCTGGACCTTGACAACCCTGAGCTGGAGACAGAAACCTCCTCAACGCACTCAGAATCTTC
NM_017559.2       GCTGGACCTTGACAACCCTGAGCTGGAGACAGAAACCTCCTCAACGCACTCAGAATCTTC
                  ************************************************************

HsCD00295970      TGTGGTCGTGGACCTGCCGGACACCCCCTTCATCTTTGAGCACACCGTCAACAATTCCAC
NM_017559.2       TGTGGTTGTGGACCTGCCGGACACCCCCTTCATCTTTGAGCACACCGTCAACAATTCCAC
                  ****** *****************************************************

HsCD00295970      AGCTGTGATTTCCTGGACCTACGCCTTGGGCAAGCAGCCGGTCAGTTTCTACCAGCTCCT
NM_017559.2       AGCTGTGATTTCCTGGACCTACGCCTTGGGCAAGCAGCCGGTCAGTTTCTACCAGCTCCT
                  ************************************************************

HsCD00295970      GTTACAGGAGGTGGCCAAGACACAGGAGAATGAGTTGCCCGAGGCAAAGAATCGTCCATG
NM_017559.2       GTTACAGGAGGTGGCCAAGACACAGGAGAATGAGTTGCCCGAGGCAAAGAATCGTCCATG
                  ************************************************************

HsCD00295970      GATCTTCAACAAGATTTTGGGCACTACTGTCAAGCTGATGGAGCTAAAGCCTAACACGTG
NM_017559.2       GATCTTCAACAAGATTTTGGGCACTACTGTCAAGCTGATGGAGCTAAAGCCTAACACGTG
                  ************************************************************

HsCD00295970      TTACTGCCTCAGTGTCCGTGCAGCCAACACAGCTGGGGTGGGGAAGTGGTGCAAGCCCTA
NM_017559.2       TTACTGCCTCAGTGTCCGTGCAGCCAACACAGCTGGGGTGGGGAAGTGGTGCAAGCCCTA
                  ************************************************************

HsCD00295970      CAAATTTGCAACCCTGGCCACTGACTTCAGCAGCTTTCCTGAGAACTACCCCATCCAGAT
NM_017559.2       CAAATTTGCAACCCTGGCCACTGACTTCAGCAGCTTTCCTGAGAACTACCCCATCCAGAT
                  ************************************************************

HsCD00295970      CACCGTGCGGCGCAAGGAACCCCGGCAAAAGATCGTGTCCATCGGGCCGGAGGAGATGCG
NM_017559.2       CACCGTGCGGCGCAAGGAACCCCGGCAAAAGATCGTGTCCATCGGGCCGGAGGAGATGCG
                  ************************************************************

HsCD00295970      GAGGCTGGAGGATCTGGAATACCTATTTCCCTGTTAGCTTGACCCAGCTTTCTTGTAC
NM_017559.2       GAGGCTGGAGGATCTGGAATACCTATTTCCCTGTTAA---------------------
                  ************************************.                     

Protein

SMART domain : FN3 : Fibronectin type 3 domain
UniProt : Q8TC99
HPRD : 13191

Clone

Cloning Information : 482312
HIP Master Clone ID : 400040
IMAGE ID : 100066629
Original Clone ID : FLH482312.01X

Organism


TAX_ID : 9606
Species Specific ID: 54752

Genome

Chromosome : 17
Map Location : 17q12
Ensembl : ENSG00000073598

Reagents

Labome : FNDC8-antibody