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P2RY5 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LPAR6
Gene Name:  lysophosphatidic acid receptor 6
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH482364.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00296022

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1035nts         Open reading frame : 23 to 1035
Reference Sequence Alignment

GTACAAAAAAGCAGGCTCCACCATGGTAAGCGTTAACAGCTCCCACTGCTTCTATAATGA
CTCCTTTAAGTACACTTTGTATGGGTGCATGTTCAGCATGGTGTTTGTGCTTGGGTTAAT
ATCCAATTGTGTTGCCATATACATTTTCATCTGCGTCCTCAAAGTCCGAAATGAAACTAC
AACTTACATGATTAACTTGGCAATGTCAGACTTGCTTTTTGTTTTTACTTTACCCTTCAG
GATTTTTTACTTCACAACACGGAATTGGCCATTTGGAGATTTACTTTGTAAGATTTCTGT
GATGCTGTTTTATACCAACATGTACGGAAGCATTCTGTTCTTAACCTGTATTAGTGTAGA
TCGATTTCTGGCAATTGTCTACCCATTTAAGTCAAAGACTCTAAGAACCAAAAGAAATGC
AAAGATTGTTTGCACTGGCGTGTGGTTAACTGTGATCGGAGGAAGTGCACCCGCCGTTTT
TGTTCAGTCTACCCACTCTCAGGGTAACAATGCCTCAGAAGCCTGCTTTGAAAATTTTCC
AGAAGCCACATGGAAAACATATCTCTCAAGGATTGTAATTTTCATCGAAATAGTGGGATT
TTTTATTCCTCTAATTTTAAATGTAACTTGTTCTAGTATGGTGCTAAAAACTTTAACCAA
ACCTGTTACATTAAGTAGAAGCAAAATAAACAAAACTAAGGTTTTAAAAATGATTTTTGT
ACATTTGATCATATTCTGTTTCTGTTTTGTTCCTTACAATATCAATCTTATTTTATATTC
TCTTGTGAGAACACAAACATTTGTTAATTGCTCAGTAGTGGCAGCAGTAAGGACAATGTA
CCCAATCACTCTCTGTATTGCTGTTTCCAACTGTTGTTTTGACCCTATAGTTTACTACTT
TACATCGGACACAATTCAGAATTCAATAAAAATGAAAAACTGGTCTGTCAGGAGAAGTGA
CTTCAGATTCTCTGAAGTTCATGGTGCAGAGAATTTTATTCAGCATAACCTACAGACCTT
AAAAAGTAAGATATTTGACAATGAATCTGCTGCCTGAATCCACCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1725
Start on reference sequence 691
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcaggctCCACC

3' Linker Sequence:
TGGATCCacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LPAR6
Symbol Nomenclature : LPAR6
SYNONYM : ARWH1; LAH3; P2RY5; P2Y5
Designation : G-protein coupled purinergic receptor P2Y5|LPA receptor 6|LPA-6|P2Y purinoceptor 5|RB intron encoded G-protein coupled receptor|oleoyl-L-alpha-lysophosphatidic acid receptor|purinergic receptor 5|purinergic receptor P2Y G protein-coupled protein 5|purinergic receptor P2Y, G-protein coupled, 5
Full Nomenclature : lysophosphatidic acid receptor 6
GENEID : 10161
GI : 190691164
GenBank Accession : EU831916
HGNC : 15520
MIM : 609239
Vega : OTTHUMG00000016895
Target GenBank: BX537392.1

 

Reference Sequence Alignment

  

HsCD00296022        GTACAAAAAAGCAGGCTCCACCATGGTAAGCGTTAACAGCTCCCACTGCTTCTATAATGA
NM_001162497.1      ----------------------ATGGTAAGCGTTAACAGCTCCCACTGCTTCTATAATGA
NM_001162498.1      ----------------------ATGGTAAGCGTTAACAGCTCCCACTGCTTCTATAATGA
NM_005767.5         ----------------------ATGGTAAGCGTTAACAGCTCCCACTGCTTCTATAATGA
                                          **************************************

HsCD00296022        CTCCTTTAAGTACACTTTGTATGGGTGCATGTTCAGCATGGTGTTTGTGCTTGGGTTAAT
NM_001162497.1      CTCCTTTAAGTACACTTTGTATGGGTGCATGTTCAGCATGGTGTTTGTGCTTGGGTTAAT
NM_001162498.1      CTCCTTTAAGTACACTTTGTATGGGTGCATGTTCAGCATGGTGTTTGTGCTTGGGTTAAT
NM_005767.5         CTCCTTTAAGTACACTTTGTATGGGTGCATGTTCAGCATGGTGTTTGTGCTTGGGTTAAT
                    ************************************************************

HsCD00296022        ATCCAATTGTGTTGCCATATACATTTTCATCTGCGTCCTCAAAGTCCGAAATGAAACTAC
NM_001162497.1      ATCCAATTGTGTTGCCATATACATTTTCATCTGCGTCCTCAAAGTCCGAAATGAAACTAC
NM_001162498.1      ATCCAATTGTGTTGCCATATACATTTTCATCTGCGTCCTCAAAGTCCGAAATGAAACTAC
NM_005767.5         ATCCAATTGTGTTGCCATATACATTTTCATCTGCGTCCTCAAAGTCCGAAATGAAACTAC
                    ************************************************************

HsCD00296022        AACTTACATGATTAACTTGGCAATGTCAGACTTGCTTTTTGTTTTTACTTTACCCTTCAG
NM_001162497.1      AACTTACATGATTAACTTGGCAATGTCAGACTTGCTTTTTGTTTTTACTTTACCCTTCAG
NM_001162498.1      AACTTACATGATTAACTTGGCAATGTCAGACTTGCTTTTTGTTTTTACTTTACCCTTCAG
NM_005767.5         AACTTACATGATTAACTTGGCAATGTCAGACTTGCTTTTTGTTTTTACTTTACCCTTCAG
                    ************************************************************

HsCD00296022        GATTTTTTACTTCACAACACGGAATTGGCCATTTGGAGATTTACTTTGTAAGATTTCTGT
NM_001162497.1      GATTTTTTACTTCACAACACGGAATTGGCCATTTGGAGATTTACTTTGTAAGATTTCTGT
NM_001162498.1      GATTTTTTACTTCACAACACGGAATTGGCCATTTGGAGATTTACTTTGTAAGATTTCTGT
NM_005767.5         GATTTTTTACTTCACAACACGGAATTGGCCATTTGGAGATTTACTTTGTAAGATTTCTGT
                    ************************************************************

HsCD00296022        GATGCTGTTTTATACCAACATGTACGGAAGCATTCTGTTCTTAACCTGTATTAGTGTAGA
NM_001162497.1      GATGCTGTTTTATACCAACATGTACGGAAGCATTCTGTTCTTAACCTGTATTAGTGTAGA
NM_001162498.1      GATGCTGTTTTATACCAACATGTACGGAAGCATTCTGTTCTTAACCTGTATTAGTGTAGA
NM_005767.5         GATGCTGTTTTATACCAACATGTACGGAAGCATTCTGTTCTTAACCTGTATTAGTGTAGA
                    ************************************************************

HsCD00296022        TCGATTTCTGGCAATTGTCTACCCATTTAAGTCAAAGACTCTAAGAACCAAAAGAAATGC
NM_001162497.1      TCGATTTCTGGCAATTGTCTACCCATTTAAGTCAAAGACTCTAAGAACCAAAAGAAATGC
NM_001162498.1      TCGATTTCTGGCAATTGTCTACCCATTTAAGTCAAAGACTCTAAGAACCAAAAGAAATGC
NM_005767.5         TCGATTTCTGGCAATTGTCTACCCATTTAAGTCAAAGACTCTAAGAACCAAAAGAAATGC
                    ************************************************************

HsCD00296022        AAAGATTGTTTGCACTGGCGTGTGGTTAACTGTGATCGGAGGAAGTGCACCCGCCGTTTT
NM_001162497.1      AAAGATTGTTTGCACTGGCGTGTGGTTAACTGTGATCGGAGGAAGTGCACCCGCCGTTTT
NM_001162498.1      AAAGATTGTTTGCACTGGCGTGTGGTTAACTGTGATCGGAGGAAGTGCACCCGCCGTTTT
NM_005767.5         AAAGATTGTTTGCACTGGCGTGTGGTTAACTGTGATCGGAGGAAGTGCACCCGCCGTTTT
                    ************************************************************

HsCD00296022        TGTTCAGTCTACCCACTCTCAGGGTAACAATGCCTCAGAAGCCTGCTTTGAAAATTTTCC
NM_001162497.1      TGTTCAGTCTACCCACTCTCAGGGTAACAATGCCTCAGAAGCCTGCTTTGAAAATTTTCC
NM_001162498.1      TGTTCAGTCTACCCACTCTCAGGGTAACAATGCCTCAGAAGCCTGCTTTGAAAATTTTCC
NM_005767.5         TGTTCAGTCTACCCACTCTCAGGGTAACAATGCCTCAGAAGCCTGCTTTGAAAATTTTCC
                    ************************************************************

HsCD00296022        AGAAGCCACATGGAAAACATATCTCTCAAGGATTGTAATTTTCATCGAAATAGTGGGATT
NM_001162497.1      AGAAGCCACATGGAAAACATATCTCTCAAGGATTGTAATTTTCATCGAAATAGTGGGATT
NM_001162498.1      AGAAGCCACATGGAAAACATATCTCTCAAGGATTGTAATTTTCATCGAAATAGTGGGATT
NM_005767.5         AGAAGCCACATGGAAAACATATCTCTCAAGGATTGTAATTTTCATCGAAATAGTGGGATT
                    ************************************************************

HsCD00296022        TTTTATTCCTCTAATTTTAAATGTAACTTGTTCTAGTATGGTGCTAAAAACTTTAACCAA
NM_001162497.1      TTTTATTCCTCTAATTTTAAATGTAACTTGTTCTAGTATGGTGCTAAAAACTTTAACCAA
NM_001162498.1      TTTTATTCCTCTAATTTTAAATGTAACTTGTTCTAGTATGGTGCTAAAAACTTTAACCAA
NM_005767.5         TTTTATTCCTCTAATTTTAAATGTAACTTGTTCTAGTATGGTGCTAAAAACTTTAACCAA
                    ************************************************************

HsCD00296022        ACCTGTTACATTAAGTAGAAGCAAAATAAACAAAACTAAGGTTTTAAAAATGATTTTTGT
NM_001162497.1      ACCTGTTACATTAAGTAGAAGCAAAATAAACAAAACTAAGGTTTTAAAAATGATTTTTGT
NM_001162498.1      ACCTGTTACATTAAGTAGAAGCAAAATAAACAAAACTAAGGTTTTAAAAATGATTTTTGT
NM_005767.5         ACCTGTTACATTAAGTAGAAGCAAAATAAACAAAACTAAGGTTTTAAAAATGATTTTTGT
                    ************************************************************

HsCD00296022        ACATTTGATCATATTCTGTTTCTGTTTTGTTCCTTACAATATCAATCTTATTTTATATTC
NM_001162497.1      ACATTTGATCATATTCTGTTTCTGTTTTGTTCCTTACAATATCAATCTTATTTTATATTC
NM_001162498.1      ACATTTGATCATATTCTGTTTCTGTTTTGTTCCTTACAATATCAATCTTATTTTATATTC
NM_005767.5         ACATTTGATCATATTCTGTTTCTGTTTTGTTCCTTACAATATCAATCTTATTTTATATTC
                    ************************************************************

HsCD00296022        TCTTGTGAGAACACAAACATTTGTTAATTGCTCAGTAGTGGCAGCAGTAAGGACAATGTA
NM_001162497.1      TCTTGTGAGAACACAAACATTTGTTAATTGCTCAGTAGTGGCAGCAGTAAGGACAATGTA
NM_001162498.1      TCTTGTGAGAACACAAACATTTGTTAATTGCTCAGTAGTGGCAGCAGTAAGGACAATGTA
NM_005767.5         TCTTGTGAGAACACAAACATTTGTTAATTGCTCAGTAGTGGCAGCAGTAAGGACAATGTA
                    ************************************************************

HsCD00296022        CCCAATCACTCTCTGTATTGCTGTTTCCAACTGTTGTTTTGACCCTATAGTTTACTACTT
NM_001162497.1      CCCAATCACTCTCTGTATTGCTGTTTCCAACTGTTGTTTTGACCCTATAGTTTACTACTT
NM_001162498.1      CCCAATCACTCTCTGTATTGCTGTTTCCAACTGTTGTTTTGACCCTATAGTTTACTACTT
NM_005767.5         CCCAATCACTCTCTGTATTGCTGTTTCCAACTGTTGTTTTGACCCTATAGTTTACTACTT
                    ************************************************************

HsCD00296022        TACATCGGACACAATTCAGAATTCAATAAAAATGAAAAACTGGTCTGTCAGGAGAAGTGA
NM_001162497.1      TACATCGGACACAATTCAGAATTCAATAAAAATGAAAAACTGGTCTGTCAGGAGAAGTGA
NM_001162498.1      TACATCGGACACAATTCAGAATTCAATAAAAATGAAAAACTGGTCTGTCAGGAGAAGTGA
NM_005767.5         TACATCGGACACAATTCAGAATTCAATAAAAATGAAAAACTGGTCTGTCAGGAGAAGTGA
                    ************************************************************

HsCD00296022        CTTCAGATTCTCTGAAGTTCATGGTGCAGAGAATTTTATTCAGCATAACCTACAGACCTT
NM_001162497.1      CTTCAGATTCTCTGAAGTTCATGGTGCAGAGAATTTTATTCAGCATAACCTACAGACCTT
NM_001162498.1      CTTCAGATTCTCTGAAGTTCATGGTGCAGAGAATTTTATTCAGCATAACCTACAGACCTT
NM_005767.5         CTTCAGATTCTCTGAAGTTCATGGTGCAGAGAATTTTATTCAGCATAACCTACAGACCTT
                    ************************************************************

HsCD00296022        AAAAAGTAAGATATTTGACAATGAATCTGCTGCCTGAATCCACCCAGCTTTCTTGTAC
NM_001162497.1      AAAAAGTAAGATATTTGACAATGAATCTGCTGCCTGA---------------------
NM_001162498.1      AAAAAGTAAGATATTTGACAATGAATCTGCTGCCTGA---------------------
NM_005767.5         AAAAAGTAAGATATTTGACAATGAATCTGCTGCCTGA---------------------
                    *************************************                     

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (q) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Gastrin-CREB signalling pathway via PKC and MAPK
Reactome : Nucleotide-like (purinergic) receptors
Reactome : P2Y receptors
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like
WikiPathway : Nucleotide GPCRs

Protein

UniProt : B3KVQ5
UniProt : F2YGU4
UniProt : P43657
HPRD : 16465

Clone

Cloning Information : 482364
HIP Master Clone ID : 400092
IMAGE ID : 100066945
Original Clone ID : FLH482364.01X

Organism


TAX_ID : 9606
Species Specific ID: 10161

Genome

Chromosome : 13
Map Location : 13q14
Ensembl : ENSG00000139679

Reagents

Labome : LPAR6-antibody