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KBTBD3 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KBTBD3
Gene Name:  kelch repeat and BTB (POZ) domain containing 3
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH482404.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00296062

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1839nts         Open reading frame : 23 to 1839
Reference Sequence Alignment

GTACAAAAAAGCAGGCTCCACCATGGAATTGGCTATGGATAATTCATACGCTTTCAATCA
ACGAAGCACGTGTAATGGAATTCCATCTGAGAAGAAAAACAACTTCCTTGTATCAGAAGA
TCATGGACAAAAAATCTTAAGTGTACTACAGAATTTTAGAGAACAAAATGTCTTTTATGA
TTTCAAAATAATTATGAAAGATGAAATAATCCCGTGTCATCGTTGTGTGTTAGCAGCATG
CAGTGACTTTTTCAGGGCTATGTTTGAAGTAAACATGAAAGAAAGAGATGATGGAAGTGT
TACCATTACTAATTTGTCCTCCAAGGCAGTAAAAGCATTTCTCGATTATGCCTATACTGG
AAAAACAAAAATAACAGATGATAATGTGGAAATGTTCTTCCAGTTGTCATCATTTCTTCA
AGTTTCCTTCCTATCCAAAGCTTGCAGTGACTTTTTAATAAAAAGTATTAATCTTGTCAA
TTGTTTACAGTTATTATCTATATCAGATAGCTATGGCTCCACCAGTTTGTTTGATCATGC
ATTACACTTTGTACAACATCACTTTTCTTTATTATTTAAATCCAGTGATTTCTTAGAGAT
GAATTTTGGAGTACTACAGAAATGTCTGGAATCAGATGAATTAAATGTTCCTGAAGAAGA
AATGGTACTGAAAGTTGTCCTTAGTTGGACTAAACATAACTTAGAATCAAGGCAAAAGTA
TCTGCCTCATTTGATTGAAAAAGTGAGATTACATCAGTTATCTGAGGAGACACTTCAGGA
CTGTCTGTTCAATGAAGAGAGTTTACTCAAAAGCACAAACTGTTTTGACATAATCATGGA
TGCAATTAAGTGTGTGCAAGGTTCTGGTGGACTCTTCCCTGATGCTCGACCATCCACAAC
TGAGAAATACATATTCATTCACAAAACTGAGGAAAATGGAGAAAATCAATATACATTTTG
CTATAACATTAAATCTGATTCATGGAAAATACTGCCGCAATCACACCTGATTGATTTGCC
AGGATCTAGTCTTTCGAGTTACGGAGAGAAAATATTCTTGACAGGTGGTTGCAAAGGGAA
ATGTTGTCGAACGGTTCGACTGCATATTGCCGAGTCATATCATGATGCCACTGATCAAAC
CTGGTGCTACTGTCCAGTGAAAAATGATTTCTTCTTGGTATCAACTATGAAAACACCAAG
AACCATGCATACATCAGTTATGGCTCTCGATAGATTATTTGTCATAGGTGGAAAAACTAG
AGGATCCCGGGACATTAAAAGTCTCTTAGATGTTGAATCTTACAATCCTCTTTCCAAAGA
ATGGATATCTGTTAGCCCATTACCCAGAGGCATATACTATCCAGAAGCAAGCACATGCCA
AAATGTAATTTATGTTCTTGGATCAGAGGTAGAGATTACAGATGCTTTTAACCCATCACT
TGATTGCTTTTTTAAATACAATGCTACAACTGATCAGTGGTCTGAACTAGTAGCAGAGTT
TGGGCAATTTTTTCATGCAACATTAATTAAAGCTGTACCAGTAAACTGTACACTGTATAT
ATGTGACCTTTCCACCTATAAGGTTTATAGTTTTTGTCCAGACACTTGTGTTTGGAAAGG
CGAAGGATCTTTTGAGTGTGCAGGCTTTAATGCAGGTGCAATTGGAATTGAAGATAAAAT
TTATATATTAGGTGGTGATTATGCACCAGATGAAATCACAGATGAAGTGCAGGTCTACCA
CAGCAACAGGTCTGAATGGGAAGAAGTTTCACCAATGCCTAGAGCCTTAACAGAATTTTA
CTGCCAGGTGATTCAGTTTAATAAATACAGAGACCCATGGTTTTCTAATCTATGTGCTTG
AATCCACCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1999
Start on reference sequence 161
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcaggctCCACC

3' Linker Sequence:
TGGATCCacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KBTBD3
Symbol Nomenclature : KBTBD3
SYNONYM : BKLHD3
Designation : BTB and kelch domain containing 3|BTB and kelch domain-containing protein 3|kelch repeat and BTB domain-containing protein 3
Full Nomenclature : kelch repeat and BTB (POZ) domain containing 3
GENEID : 143879
GI : 190691174
GenBank Accession : EU831921
HGNC : 22934
Vega : OTTHUMG00000166251
Target GenBank: BX640672.1

 

Reference Sequence Alignment

  

HsCD00296062      GTACAAAAAAGCAGGCTCCACCATGGAATTGGCTATGGATAATTCATACGCTTTCAATCA
NM_198439.2       ----------------------ATGGAATTGGCTATGGATAATTCATATGCTTTCAATCA
NM_152433.3       ----------------------ATGGAATTGGCTATGGATAATTCATATGCTTTCAATCA
                                        ************************** ***********

HsCD00296062      ACGAAGCACGTGTAATGGAATTCCATCTGAGAAGAAAAACAACTTCCTTGTATCAGAAGA
NM_198439.2       ACGAAGCACATGTAATGGAATTCCATCTGAGAAGAAAAACAACTTCCTTGTATCAGAAGA
NM_152433.3       ACGAAGCACATGTAATGGAATTCCATCTGAGAAGAAAAACAACTTCCTTGTATCAGAAGA
                  *********.**************************************************

HsCD00296062      TCATGGACAAAAAATCTTAAGTGTACTACAGAATTTTAGAGAACAAAATGTCTTTTATGA
NM_198439.2       TCATGGACAAAAAATCTTAAGTGTACTACAGAATTTTAGAGAACAAAATGTCTTTTATGA
NM_152433.3       TCATGGACAAAAAATCTTAAGTGTACTACAGAATTTTAGAGAACAAAATGTCTTTTATGA
                  ************************************************************

HsCD00296062      TTTCAAAATAATTATGAAAGATGAAATAATCCCGTGTCATCGTTGTGTGTTAGCAGCATG
NM_198439.2       TTTCAAAATAATTATGAAAGATGAAATAATCCCGTGTCATCGTTGTGTGTTAGCAGCATG
NM_152433.3       TTTCAAAATAATTATGAAAGATGAAATAATCCCGTGTCATCGTTGTGTGTTAGCAGCATG
                  ************************************************************

HsCD00296062      CAGTGACTTTTTCAGGGCTATGTTTGAAGTAAACATGAAAGAAAGAGATGATGGAAGTGT
NM_198439.2       CAGTGACTTTTTCAGGGCTATGTTTGAAGTAAACATGAAAGAAAGAGATGATGGAAGTGT
NM_152433.3       CAGTGACTTTTTCAGGGCTATGTTTGAAGTAAACATGAAAGAAAGAGATGATGGAAGTGT
                  ************************************************************

HsCD00296062      TACCATTACTAATTTGTCCTCCAAGGCAGTAAAAGCATTTCTCGATTATGCCTATACTGG
NM_198439.2       TACCATTACTAATTTGTCCTCCAAGGCAGTAAAAGCATTTCTCGATTATGCCTATACTGG
NM_152433.3       TACCATTACTAATTTGTCCTCCAAGGCAGTAAAAGCATTTCTCGATTATGCCTATACTGG
                  ************************************************************

HsCD00296062      AAAAACAAAAATAACAGATGATAATGTGGAAATGTTCTTCCAGTTGTCATCATTTCTTCA
NM_198439.2       AAAAACAAAAATAACAGATGATAATGTGGAAATGTTCTTCCAGTTGTCATCATTTCTTCA
NM_152433.3       AAAAACAAAAATAACAGATGATAATGTGGAAATGTTCTTCCAGTTGTCATCATTTCTTCA
                  ************************************************************

HsCD00296062      AGTTTCCTTCCTATCCAAAGCTTGCAGTGACTTTTTAATAAAAAGTATTAATCTTGTCAA
NM_198439.2       AGTTTCCTTCCTATCCAAAGCTTGCAGTGACTTTTTAATAAAAAGTATTAATCTTGTCAA
NM_152433.3       AGTTTCCTTCCTATCCAAAGCTTGCAGTGACTTTTTAATAAAAAGTATTAATCTTGTCAA
                  ************************************************************

HsCD00296062      TTGTTTACAGTTATTATCTATATCAGATAGCTATGGCTCCACCAGTTTGTTTGATCATGC
NM_198439.2       TTGTTTACAGTTATTATCTATATCAGATAGCTATGGCTCCACCAGTTTGTTTGATCATGC
NM_152433.3       TTGTTTACAGTTATTATCTATATCAGATAGCTATGGCTCCACCAGTTTGTTTGATCATGC
                  ************************************************************

HsCD00296062      ATTACACTTTGTACAACATCACTTTTCTTTATTATTTAAATCCAGTGATTTCTTAGAGAT
NM_198439.2       ATTACACTTTGTACAACATCACTTTTCTTTATTATTTAAATCCAGTGATTTCTTAGAGAT
NM_152433.3       ATTACACTTTGTACAACATCACTTTTCTTTATTATTTAAATCCAGTGATTTCTTAGAGAT
                  ************************************************************

HsCD00296062      GAATTTTGGAGTACTACAGAAATGTCTGGAATCAGATGAATTAAATGTTCCTGAAGAAGA
NM_198439.2       GAATTTTGGAGTACTACAGAAATGTCTGGAATCAGATGAATTAAATGTTCCTGAAGAAGA
NM_152433.3       GAATTTTGGAGTACTACAGAAATGTCTGGAATCAGATGAATTAAATGTTCCTGAAGAAGA
                  ************************************************************

HsCD00296062      AATGGTACTGAAAGTTGTCCTTAGTTGGACTAAACATAACTTAGAATCAAGGCAAAAGTA
NM_198439.2       AATGGTACTGAAAGTTGTCCTTAGTTGGACTAAACATAACTTAGAATCAAGGCAAAAGTA
NM_152433.3       AATGGTACTGAAAGTTGTCCTTAGTTGGACTAAACATAACTTAGAATCAAGGCAAAAGTA
                  ************************************************************

HsCD00296062      TCTGCCTCATTTGATTGAAAAAGTGAGATTACATCAGTTATCTGAGGAGACACTTCAGGA
NM_198439.2       TCTGCCTCATTTGATTGAAAAAGTGAGATTACATCAGTTATCTGAGGAGACACTTCAGGA
NM_152433.3       TCTGCCTCATTTGATTGAAAAAGTGAGATTACATCAGTTATCTGAGGAGACACTTCAGGA
                  ************************************************************

HsCD00296062      CTGTCTGTTCAATGAAGAGAGTTTACTCAAAAGCACAAACTGTTTTGACATAATCATGGA
NM_198439.2       CTGTCTGTTCAATGAAGAGAGTTTACTCAAAAGCACAAACTGTTTTGACATAATCATGGA
NM_152433.3       CTGTCTGTTCAATGAAGAGAGTTTACTCAAAAGCACAAACTGTTTTGACATAATCATGGA
                  ************************************************************

HsCD00296062      TGCAATTAAGTGTGTGCAAGGTTCTGGTGGACTCTTCCCTGATGCTCGACCATCCACAAC
NM_198439.2       TGCAATTAAGTGTGTGCAAGGTTCTGGTGGACTCTTCCCTGATGCTCGACCATCCACAAC
NM_152433.3       TGCAATTAAGTGTGTGCAAGGTTCTGGTGGACTCTTCCCTGATGCTCGACCATCCACAAC
                  ************************************************************

HsCD00296062      TGAGAAATACATATTCATTCACAAAACTGAGGAAAATGGAGAAAATCAATATACATTTTG
NM_198439.2       TGAGAAATACATATTCATTCACAAAACTGAGGAAAATGGAGAAAATCAATATACATTTTG
NM_152433.3       TGAGAAATACATATTCATTCACAAAACTGAGGAAAATGGAGAAAATCAATATACATTTTG
                  ************************************************************

HsCD00296062      CTATAACATTAAATCTGATTCATGGAAAATACTGCCGCAATCACACCTGATTGATTTGCC
NM_198439.2       CTATAACATTAAATCTGATTCATGGAAAATACTGCCGCAATCACACCTGATTGATTTGCC
NM_152433.3       CTATAACATTAAATCTGATTCATGGAAAATACTGCCGCAATCACACCTGATTGATTTGCC
                  ************************************************************

HsCD00296062      AGGATCTAGTCTTTCGAGTTACGGAGAGAAAATATTCTTGACAGGTGGTTGCAAAGGGAA
NM_198439.2       AGGATCTAGTCTTTCGAGTTACGGAGAGAAAATATTCTTGACAGGTGGTTGCAAAGGGAA
NM_152433.3       AGGATCTAGTCTTTCGAGTTACGGAGAGAAAATATTCTTGACAGGTGGTTGCAAAGGGAA
                  ************************************************************

HsCD00296062      ATGTTGTCGAACGGTTCGACTGCATATTGCCGAGTCATATCATGATGCCACTGATCAAAC
NM_198439.2       ATGTTGTCGAACGGTTCGACTGCATATTGCCGAGTCATATCATGATGCCACTGATCAAAC
NM_152433.3       ATGTTGTCGAACGGTTCGACTGCATATTGCCGAGTCATATCATGATGCCACTGATCAAAC
                  ************************************************************

HsCD00296062      CTGGTGCTACTGTCCAGTGAAAAATGATTTCTTCTTGGTATCAACTATGAAAACACCAAG
NM_198439.2       CTGGTGCTACTGTCCAGTGAAAAATGATTTCTTCTTGGTATCAACTATGAAAACACCAAG
NM_152433.3       CTGGTGCTACTGTCCAGTGAAAAATGATTTCTTCTTGGTATCAACTATGAAAACACCAAG
                  ************************************************************

HsCD00296062      AACCATGCATACATCAGTTATGGCTCTCGATAGATTATTTGTCATAGGTGGAAAAACTAG
NM_198439.2       AACCATGCATACATCAGTTATGGCTCTCGATAGATTATTTGTCATAGGTGGAAAAACTAG
NM_152433.3       AACCATGCATACATCAGTTATGGCTCTCGATAGATTATTTGTCATAGGTGGAAAAACTAG
                  ************************************************************

HsCD00296062      AGGATCCCGGGACATTAAAAGTCTCTTAGATGTTGAATCTTACAATCCTCTTTCCAAAGA
NM_198439.2       AGGATCCCGGGACATTAAAAGTCTCTTAGATGTTGAATCTTACAATCCTCTTTCCAAAGA
NM_152433.3       AGGATCCCGGGACATTAAAAGTCTCTTAGATGTTGAATCTTACAATCCTCTTTCCAAAGA
                  ************************************************************

HsCD00296062      ATGGATATCTGTTAGCCCATTACCCAGAGGCATATACTATCCAGAAGCAAGCACATGCCA
NM_198439.2       ATGGATATCTGTTAGCCCATTACCCAGAGGCATATACTATCCAGAAGCAAGCACATGCCA
NM_152433.3       ATGGATATCTGTTAGCCCATTACCCAGAGGCATATACTATCCAGAAGCAAGCACATGCCA
                  ************************************************************

HsCD00296062      AAATGTAATTTATGTTCTTGGATCAGAGGTAGAGATTACAGATGCTTTTAACCCATCACT
NM_198439.2       AAATGTAATTTATGTTCTTGGATCAGAGGTAGAGATTACAGATGCTTTTAACCCATCACT
NM_152433.3       AAATGTAATTTATGTTCTTGGATCAGAGGTAGAGATTACAGATGCTTTTAACCCATCACT
                  ************************************************************

HsCD00296062      TGATTGCTTTTTTAAATACAATGCTACAACTGATCAGTGGTCTGAACTAGTAGCAGAGTT
NM_198439.2       TGATTGCTTTTTTAAATACAATGCTACAACTGATCAGTGGTCTGAACTAGTAGCAGAGTT
NM_152433.3       TGATTGCTTTTTTAAATACAATGCTACAACTGATCAGTGGTCTGAACTAGTAGCAGAGTT
                  ************************************************************

HsCD00296062      TGGGCAATTTTTTCATGCAACATTAATTAAAGCTGTACCAGTAAACTGTACACTGTATAT
NM_198439.2       TGGGCAATTTTTTCATGCAACATTAATTAAAGCTGTACCAGTAAACTGTACACTGTATAT
NM_152433.3       TGGGCAATTTTTTCATGCAACATTAATTAAAGCTGTACCAGTAAACTGTACACTGTATAT
                  ************************************************************

HsCD00296062      ATGTGACCTTTCCACCTATAAGGTTTATAGTTTTTGTCCAGACACTTGTGTTTGGAAAGG
NM_198439.2       ATGTGACCTTTCCACCTATAAGGTTTATAGTTTTTGTCCAGACACTTGTGTTTGGAAAGG
NM_152433.3       ATGTGACCTTTCCACCTATAAGGTTTATAGTTTTTGTCCAGACACTTGTGTTTGGAAAGG
                  ************************************************************

HsCD00296062      CGAAGGATCTTTTGAGTGTGCAGGCTTTAATGCAGGTGCAATTGGAATTGAAGATAAAAT
NM_198439.2       CGAAGGATCTTTTGAGTGTGCAGGCTTTAATGCAGGTGCAATTGGAATTGAAGATAAAAT
NM_152433.3       CGAAGGATCTTTTGAGTGTGCAGGCTTTAATGCAGGTGCAATTGGAATTGAAGATAAAAT
                  ************************************************************

HsCD00296062      TTATATATTAGGTGGTGATTATGCACCAGATGAAATCACAGATGAAGTGCAGGTCTACCA
NM_198439.2       TTATATATTAGGTGGTGATTATGCACCAGATGAAATCACAGATGAAGTGCAGGTCTACCA
NM_152433.3       TTATATATTAGGTGGTGATTATGCACCAGATGAAATCACAGATGAAGTGCAGGTCTACCA
                  ************************************************************

HsCD00296062      CAGCAACAGGTCTGAATGGGAAGAAGTTTCACCAATGCCTAGAGCCTTAACAGAATTTTA
NM_198439.2       CAGCAACAGGTCTGAATGGGAAGAAGTTTCACCAATGCCTAGAGCCTTAACAGAATTTTA
NM_152433.3       CAGCAACAGGTCTGAATGGGAAGAAGTTTCACCAATGCCTAGAGCCTTAACAGAATTTTA
                  ************************************************************

HsCD00296062      CTGCCAGGTGATTCAGTTTAATAAATACAGAGACCCATGGTTTTCTAATCTATGTGCTTG
NM_198439.2       CTGCCAGGTGATTCAGTTTAATAAATACAGAGACCCATGGTTTTCTAATCTATGTGCTTG
NM_152433.3       CTGCCAGGTGATTCAGTTTAATAAATACAGAGACCCATGGTTTTCTAATCTATGTGCTTG
                  ************************************************************

HsCD00296062      AATCCACCCAGCTTTCTTGTAC
NM_198439.2       A---------------------
NM_152433.3       A---------------------
                  *                     

Protein

SMART domain : Kelch :
SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
UniProt : A8K1K0
UniProt : Q8NAB2
HPRD : 10005

Clone

Cloning Information : 482404
HIP Master Clone ID : 400132
IMAGE ID : 100066950
Original Clone ID : FLH482404.01X

Organism


TAX_ID : 9606
Species Specific ID: 143879

Genome

Chromosome : 11
Map Location : 11q22.3
Ensembl : ENSG00000182359

Reagents

Labome : KBTBD3-antibody