Gene:
Gene Symbol:
TJP1
Gene Name:
tight junction protein 1
Original Clone ID:
FLH482600.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00296258
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGAGAAATACGTTTTCAATGGATTGCCACAGTAAGAGCACAGCAATGGAGGAAACA
GCTATATGGGAACAACATACAGTGACGCTTCACAGGGCTCCTGGATTTGGATTTGGAATT
GCAATATCTGGTGGACGAGATAATCCTCATTTTCAGAGTGGGGAAACGTCAATAGTGATT
TCAGATGTGCTGAAAGGAGGACCAGCTGAAGGACAGCTACAGGAAAATGACCGAGTTGCA
ATGGTTAACGGAGTTTCAATGGATAATGTTGAACATGCTTTTGCTGTTCAGCAACTAAGG
AAAAGTGGGAAAAATGCAAAAATTACAATTAGAAGGAAGAAGAAAGTTCAAATACCAGTA
AGCCGTCCTGATCCTGAACCAGTATCTGATAATGAAGAAGATAGTTATGATGAGGAAATA
CATGATCCAAGAAGTGGCCGGAGTGGTGTGGTTAACAGAAGGAGTGAGAAGATTTGGCCG
AGGGATAGAAGTGCAAGTAGAGAGAGGAGCTTGTCCCCGCGGTCAGACAGGCGGTCAGTG
GCTTCCAGCCAGCCTGCTAAACCTACTAAAGTCACACTGGTGAAATCCCGGAAAAATGAA
GAATATGGTCTTCGATTGGCAAGCCATATATTTGTTAAGGAAATTTCACAAGATAGTTTG
GCAGCAAGAGATGGCAATATTCAAGAAGGTGATGTTGTATTGAAGATAAATGGTACTGTG
ACAGAAAATATGTCATTGACAGATGCAAAGACATTGATAGAAAGGTCTAAAGGCAAATTA
AAAATGGTAGTTCAAAGAGATGAACGGGCTACGCTATTGAATGTCCCTGATCTTTCTGAC
AGCATCCACTCTGCTAATGCCTCTGAGAGAGACGACATTTCAGAAATTCAGTCACTGGCA
TCAGATCATTCTGGTCGATCACACGATAGGCCTCCCCGCCGCAGCCGGTCACGATCTCCT
GACCAGCGGTCAGAGCCTTCTGATCATTCCAGGCACTCGCCGCAGCAGCCAAGCAATGGC
AGTCTCCGGAGTAGAGATGAAGAGAGAATTTCTAAACCTGGGGCTGTCTCAACTCCTGTA
AAGCATGCTGATGATCACACACCTAAAACAGTGGAAGAAGTTACAGTTGAAAGAAATGAG
AAACAAACACCTTCTCTTCCAGAACCAAAGCCTGTGTATGCCCAAGTTGGGCAACCAGAT
GTGGATTTACCTGTCAGTCCATCTGATGGTGTCCTACCTAATTCAACTCATGAAGATGGG
ATTCTTCGGCCCAGCATGAAATTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGG
CTGGCTGGTGGAAATGATGTTGGAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCA
GCCAAGGAAGGCTTAGAGGAAGGTGATCAAATTCTCAGGGTAAGCAACGTAGATTTTACA
AATATCATAAGAGAAGAAGCCGTCCTTTTCCTGCTTGACCTCCCTAAAGGAGAAGAAGTG
ACCATATTGGCTCAGAAGAAGAAGGATGTTTATCGTCGCATTGTAGAATCAGATGTAGGA
GATTCTTTCTATATTAGAACCCATTTTGAATATGAAAAGGAATCTCCCTATGGACTTAGT
TTTAACAAAGGAGAGGTGTTCCGTGTTGTGGATACCTTGTACAATGGAAAACTGGGCTCT
TGGCTTGCTATTCGAATTGGTAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAAT
AAGAACAGAGCTGAGCAGCTAGCCAGTGTACAGTATACACTTCCAAAAACAGCAGGCGGA
GACCGTGCTGACTTCTGGAGATTCAGAGGTCTTCGCAGCTCCAAGAGAAATCTTCGAAAA
AGCAGAGAGGATTTGTCCGCTCAGCCTGTTCAAACAAAGTTTCCAGCTTATGAAAGAGTG
GTTCTTCGAGAAGCTGGATTTCTGAGGCCTGTAACCATTTTTGGACCAATAGCTGATGTT
GCCAGAGAAAAGCTGGCAAGAGAAGAACCAGATATTTATCAAATTGCAAAGAGTGAACCA
CGAGACGCTGGAACTGACCAACGTAGCTCTGGCATTATTCGCCTGCATACAATAAAGCAA
ATCATAGATCAAGGCAAACATGCTTTATTAGATGTAACACCAAATGCAGTTGATCGTCTT
AACTATGCCCAGTGGTATCCAATTGTTGTATTTCTTAACCCTGATTCTAAGCAAGGAGTA
AAAACAATGAGAATGAGGTTATGTCTAGAATCTCGGAAAAGTGCCAGGAAGTTATACGAG
CGATCTCATAAACTTCGTAAAAATAATCACCATCTTTTTACAACTACAATTAACTTAAAT
TCAATGAATGATGGTTGGTATGGTGCGCTGAAAGAAGCAATTCAACAACAGCAAAACCAG
CTGGTATGGGTTTCCGAGGGAAAGGCGGATGGTGCTACAAGTGATGACCTTGATTTGCAT
GATGATCGTCTGTCCTACCTGTCAGCTCCAGGTAGTGAATACTCAATGTATAGCACGGAC
AGTAGACACACTTCTGACTATGAAGACACAGACACAGAAGGCGGGGCCTACACTGATCAA
GAACTAGATGAAACTCTTAATGATGAGGTTGGGACTCCACCGGAGTCTGCCATTACACGG
TCCTCTGAGCCTGTAAGAGAGGACTCCTCTGGAATGCATCATGAAAACCAAACATATCCT
CCTTACTCACCACAAGCGCAGCCACAACCAATTCATAGAATAGACTCCCCTGGATTTAAG
CCAGCCTCTCAACAGGTGTATAGAAAGGATCCATATCCCGAGGAAATGATGAGGCAGAAC
CATGTTTTGAAACAGCCAGCCGTTAGTCACCCAGGGCACAGGCCAGACAAAGAGCCTAAT
CTGACCTATGAACCCCAACTCCCATACGTAGAGAAACAAGCCAGCAGAGACCTCGAGCAG
CCCACATACAGATACGAGTCCTCAAGCTATACGGACCAGTTTTCTCGAAACTATGAACAT
CGTCTGCGATACGAAGATCGCGTCCCCATGTATGAAGAACAGTGGTCATATTATGATGAC
AAACAGCCCTACCCATCTCGGCCACCTTTTGATAATCAGCACTCTCAAGACCTTGACTCC
AGACAGCATCCCGAAGAGTCCTCAGAACGAGGGTACTTTCCACGTTTTGAAGAGCCAGCC
CCTCTGTCTTACGACAGCAGACCACGTTACGAACAGGCACCTAGAGCATCCGCCCTGCGG
CACGAAGAGCAGCCAGCTCCTGGGTATGACACACATGGTAGACTCAGACCGGAAGCCCAG
CCCCACCCTTCAGCAGGGCCCAAGCCTGCAGAGTCCAAGCAGTATTTTGAGCAATATTCA
CGCAGTTACGAGCAAGTACCACCCCAAGGATTTACCTCTAGAGCAGGTCATTTTGAGCCT
CTCCATGGTGCTGCAGCTGTCCCTCCGCTGATACCTTCATCTCAGCATAAGCCAGAAGCT
CTGCCTTCAAACACCAAACCACTGCCTCCACCCCCAACTCAAACCGAAGAAGAGGAAGAT
CCAGCAATGAAGCCACAGTCTGTACTCACCAGAGTTAAGATGTTTGAAAACAAAAGATCT
GCATCCTTAGAGACCAAGAAGGATGTAAATGACACTGGCAGTTTTAAGCCTCCAGAAGTA
GCATCTAAACCTTCAGGTGCTCCCATCATTGGTCCCAAACCCACTTCTCAGAATCAATTC
AGTGAACATGACAAAACTCTGTACAGGGTCCCAGAACCTCAAAAACCTCAACTGAAGCCA
CCTGAAGATATTGTTCGGTCCAATCATTATGACCCTGAAGAAGATGAAGAATATTATCGA
AAACAGCTGTCATACTTTGACCGAAGAAGTTTTGAGAATAAGCCTCCTGCACACATTGCT
GCCAGCCATCTCTCCGAGCCTGCAAAGCTAGCTCATTCTCAGAATCAATCAAATTTTTCT
AGTTATTCTTCAAAGGGAAAGCCTCCTGAAGCTGATGGTGTGGATAGATCATTTGGCGAG
AAACGCTATGAACCCATCCAGGCCACTCCCCCTCCTCCTCCATTGCCCTCGCAGTATGCC
CAGCCATCTCAGCCTGTCACCAGCGCGTCTCTCCACATACATTCTAAGGGAGCACATGGT
GAAGGTAATTCAGTGTCATTGGATTTTCAGAATTCCTTAGTGTCCAAACCAGACCCACCT
CCATCTCAGAATAAGCCAGCAACTTTCAGACCACCAAACCGAGAAGATACTGCTCAGGCA
GCTTTCTATCCCCAGAAAAGTTTTCCAGATAAAGCCCCAGTTAATGGAACTGAACAGACT
CAGAAAACAGTCACTCCAGCATACAATCGATTCACACCAAAACCATATACAAGTTCTGCC
CGACCATTTGAACGCAAGTTTGAAAGTCCTAAATTCAATCACAATCTTCTGCCAAGTGAA
ACTGCACATAAACCTGACTTGTCTTCAAAAACTCCCACTTCTCCAAAAACTCTTGTGAAA
TCGCACAGTTTGGCACAGCCTCCTGAGTTTGACAGTGGAGTTGAAACTTTCTCTATCCAT
GCAGAGAAGCCTAAATATCAAATAAATAATATCAGCACAGTGCCTAAAGCTATTCCTGTG
AGTCCTTCAGCTGTGGAAGAGGATGAAGATGAAGATGGTCATACTGTGGTGGCCACAGCC
CGAGGCATATTTAACAGCAATGGGGGCGTGCTGAGTTCCATAGAAACTGGTGTTAGTATA
ATTATCCCTCAAGGAGCCATTCCCGAAGGAGTTGAGCAGGAAATCTATTTCAAGGTCTGC
CGGGACAACAGCATCCTTCCACCTTTAGATAAAGAGAAAGGTGAAACACTGCTGAGTCCT
TTGGTGATGTGTGGTCCCCATGGCCTCAAGTTCCTGAAGCCTGTGGAGCTGCGCTTACCA
CACTGTGCGTCCATGACTCCTGCCGGTTGGTCTTTTGCTCTAAAATCATCCGACTCCTCG
TCGGGTGATCCTAAAACCTGGCAAAACAAGCGTCTTCCCGGAGATCCAAATTATCTCGTT
GGAGCAAACTGTGTTTCTGTCCTTATTGACCACTTT
Protein sequence predicted based on nucleotide sequence
MERNTFSMDCHSKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVI SDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPV SRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSV ASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTV TENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLA SDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPV KHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDG ILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVSNVDFT NIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLS FNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGG DRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADV AREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQGKHALLDVTPNAVDRL NYAQWYPIVVFLNPDSKQGVKTMRMRLCLESRKSARKLYERSHKLRKNNHHLFTTTINLN SMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTD SRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYP PYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPN LTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDD KQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALR HEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEP LHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRS ASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRVPEPQKPQLKP PEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKLAHSQNQSNFS SYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHG EGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQT QKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVK SHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATA RGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSP LVMCGPHGLKFLKPVELRLPHCASMTPAGWSFALKSSDSSSGDPKTWQNKRLPGDPNYLV GANCVSVLIDHF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
244
End on reference sequence
5322
Mutations:
None
Discrepancy:
t979c,S327P; a1579g,T527A
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
