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CYTH1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CYTH1
Gene Name:  cytohesin 1
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH482663.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00296321

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1197nts         Open reading frame : 23 to 1197
Reference Sequence Alignment

GTACAAAAAAGCAGGCTCCACCATGGAGGAGGACGACAGCTACGTTCCCAGTGACCTGAC
AGCAGAGGAGCGTCAAGAACTGGAGAACATCCGACGGAGAAAACAGGAGCTGCTGGCTGA
CATTCAGAGGCTGAAGGATGAGATAGCAGAAGTAGCTAATGAAATTGAAAACCTGGGATC
CACAGAGGAAAGGAAAAACATGCAGAGGAACAAACAGGTAGCCATGGGCAGGAAAAAATT
TAATATGGACCCTAAAAAGGGGATCCAGTTCTTAATAGAGAACGACCTCCTGAAGAACAC
TTGTGAAGACATTGCCCAGTTCTTATATAAAGGCGAAGGGCTCAACAAGACAGCCATCGG
CGACTACCTAGGGGAGAGAGATGAGTTTAATATCCAGGTTCTTCATGCATTTGTGGAGCT
GCATGAGTTCACTGATCTTAATCTCGTCCAGGCACTACGGCAGTTCCTGTGGAGCTTCCG
GCTACCCGGAGAGGCCCAGAAGATCGACCGGATGATGGAGGCGTTTGCCCAGCGATATTG
TCAGTGCAATAATGGCGTGTTCCAGTCCACGGATACTTGTTACGTCCTCTCCTTTGCCAT
CATCATGTTGAACACCAGTCTGCACAACCCCAATGTCAAAGATAAGCCCACTGTGGAGAG
GTTCATTGCCATGAACCGAGGCATCAATGATGGGGGAGACCTGCCGGAGGAGCTCCTCCG
GAATCTCTATGAGAGCATAAAAAATGAACCCTTTAAAATCCCAGAAGACGACGGGAATGA
CCTCACTCACACTTTCTTCAATCCAGACCGAGAAGGCTGGCTATTGAAACTCGGAGGTGG
CAGGGTAAAGACTTGGAAGAGACGCTGGTTCATTCTGACTGACAACTGCCTTTACTACTT
TGAGTATACCACGGATAAGGAGCCCCGTGGAATCATCCCTTTAGAGAATCTGAGTATCCG
GGAAGTGGAGGACTCCAAAAAACCAAACTGCTTTGAGCTTTATATCCCCGACAATAAAGA
CCAAGTTATCAAGGCCTGCAAGACCGAGGCTGACGGGCGGGTGGTGGAGGGGAACCACAC
TGTTTACCGGATCTCAGCTCCGACGCCCGAGGAGAAGGAGGAGTGGATTAAGTGCATTAA
AGCAGCCATCAGCAGGGACCCTTTCTACGAAATGCTCGCAGCACGGAAAAAGAAGGTCTC
CTCCACGAAGCGACACTGAATCCACCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1237
Start on reference sequence 41
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcaggctCCACC

3' Linker Sequence:
TGGATCCacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CYTH1
Symbol Nomenclature : CYTH1
SYNONYM : B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7
Designation : PH, SEC7 and coiled-coil domain-containing protein 1|SEC7 homolog B2-1|cytoadhesin 1|cytohesin-1|homolog of secretory protein SEC7|pleckstrin homology, Sec7 and coiled-coil domains 1
Full Nomenclature : cytohesin 1
GENEID : 9267
GI : 190691576
GenBank Accession : EU832306
HGNC : 9501
MIM : 182115
Vega : OTTHUMG00000150253
Target GenBank: BC038385.1

 

Reference Sequence Alignment

  

HsCD00296321      GTACAAAAAAGCAGGCTCCACCATGGAGGAGGACGACAGCTACGTTCCCAGTGACCTGAC
NM_017456.2       ----------------------ATGGAGGAGGACGACAGCTACGTTCCCAGTGACCTGAC
NM_004762.2       ----------------------ATGGAGGAGGACGACAGCTACGTTCCCAGTGACCTGAC
                                        **************************************

HsCD00296321      AGCAGAGGAGCGTCAAGAACTGGAGAACATCCGACGGAGAAAACAGGAGCTGCTGGCTGA
NM_017456.2       AGCAGAGGAGCGTCAAGAACTGGAGAACATCCGACGGAGAAAACAGGAGCTGCTGGCTGA
NM_004762.2       AGCAGAGGAGCGTCAAGAACTGGAGAACATCCGACGGAGAAAACAGGAGCTGCTGGCTGA
                  ************************************************************

HsCD00296321      CATTCAGAGGCTGAAGGATGAGATAGCAGAAGTAGCTAATGAAATTGAAAACCTGGGATC
NM_017456.2       CATTCAGAGGCTGAAGGATGAGATAGCAGAAGTAGCTAATGAAATTGAAAACCTGGGATC
NM_004762.2       CATTCAGAGGCTGAAGGATGAGATAGCAGAAGTAGCTAATGAAATTGAAAACCTGGGATC
                  ************************************************************

HsCD00296321      CACAGAGGAAAGGAAAAACATGCAGAGGAACAAACAGGTAGCCATGGGCAGGAAAAAATT
NM_017456.2       CACAGAGGAAAGGAAAAACATGCAGAGGAACAAACAGGTAGCCATGGGCAGGAAAAAATT
NM_004762.2       CACAGAGGAAAGGAAAAACATGCAGAGGAACAAACAGGTAGCCATGGGCAGGAAAAAATT
                  ************************************************************

HsCD00296321      TAATATGGACCCTAAAAAGGGGATCCAGTTCTTAATAGAGAACGACCTCCTGAAGAACAC
NM_017456.2       TAATATGGACCCTAAAAAGGGGATCCAGTTCTTAATAGAGAACGACCTCCTGAAGAACAC
NM_004762.2       TAATATGGACCCTAAAAAGGGGATCCAGTTCTTAATAGAGAACGACCTCCTGAAGAACAC
                  ************************************************************

HsCD00296321      TTGTGAAGACATTGCCCAGTTCTTATATAAAGGCGAAGGGCTCAACAAGACAGCCATCGG
NM_017456.2       TTGTGAAGACATTGCCCAGTTCTTATATAAAGGCGAAGGGCTCAACAAGACAGCCATCGG
NM_004762.2       TTGTGAAGACATTGCCCAGTTCTTATATAAAGGCGAAGGGCTCAACAAGACAGCCATCGG
                  ************************************************************

HsCD00296321      CGACTACCTAGGGGAGAGAGATGAGTTTAATATCCAGGTTCTTCATGCATTTGTGGAGCT
NM_017456.2       CGACTACCTAGGGGAGAGAGATGAGTTTAATATCCAGGTTCTTCATGCATTTGTGGAGCT
NM_004762.2       CGACTACCTAGGGGAGAGAGATGAGTTTAATATCCAGGTTCTTCATGCATTTGTGGAGCT
                  ************************************************************

HsCD00296321      GCATGAGTTCACTGATCTTAATCTCGTCCAGGCACTACGGCAGTTCCTGTGGAGCTTCCG
NM_017456.2       GCATGAGTTCACTGATCTTAATCTCGTCCAGGCACTACGGCAGTTCCTGTGGAGCTTCCG
NM_004762.2       GCATGAGTTCACTGATCTTAATCTCGTCCAGGCACTACGGCAGTTCCTGTGGAGCTTCCG
                  ************************************************************

HsCD00296321      GCTACCCGGAGAGGCCCAGAAGATCGACCGGATGATGGAGGCGTTTGCCCAGCGATATTG
NM_017456.2       GCTACCCGGAGAGGCCCAGAAGATCGACCGGATGATGGAGGCGTTTGCCCAGCGATATTG
NM_004762.2       GCTACCCGGAGAGGCCCAGAAGATCGACCGGATGATGGAGGCGTTTGCCCAGCGATATTG
                  ************************************************************

HsCD00296321      TCAGTGCAATAATGGCGTGTTCCAGTCCACGGATACTTGTTACGTCCTCTCCTTTGCCAT
NM_017456.2       TCAGTGCAATAATGGCGTGTTCCAGTCCACGGATACTTGTTACGTCCTCTCCTTTGCCAT
NM_004762.2       TCAGTGCAATAATGGCGTGTTCCAGTCCACGGATACTTGTTACGTCCTCTCCTTTGCCAT
                  ************************************************************

HsCD00296321      CATCATGTTGAACACCAGTCTGCACAACCCCAATGTCAAAGATAAGCCCACTGTGGAGAG
NM_017456.2       CATCATGTTGAACACCAGTCTGCACAACCCCAATGTCAAAGATAAGCCCACTGTGGAGAG
NM_004762.2       CATCATGTTGAACACCAGTCTGCACAACCCCAATGTCAAAGATAAGCCCACTGTGGAGAG
                  ************************************************************

HsCD00296321      GTTCATTGCCATGAACCGAGGCATCAATGATGGGGGAGACCTGCCGGAGGAGCTCCTCCG
NM_017456.2       GTTCATTGCCATGAACCGAGGCATCAATGATGGGGGAGACCTGCCGGAGGAGCTCCTCCG
NM_004762.2       GTTCATTGCCATGAACCGAGGCATCAATGATGGGGGAGACCTGCCGGAGGAGCTCCTCCG
                  ************************************************************

HsCD00296321      GAATCTCTATGAGAGCATAAAAAATGAACCCTTTAAAATCCCAGAAGACGACGGGAATGA
NM_017456.2       GAATCTCTATGAGAGCATAAAAAATGAACCCTTTAAAATCCCAGAAGACGACGGGAATGA
NM_004762.2       GAATCTCTATGAGAGCATAAAAAATGAACCCTTTAAAATCCCAGAAGACGACGGGAATGA
                  ************************************************************

HsCD00296321      CCTCACTCACACTTTCTTCAATCCAGACCGAGAAGGCTGGCTATTGAAACTCGGAGGTGG
NM_017456.2       CCTCACTCACACTTTCTTCAATCCAGACCGAGAAGGCTGGCTATTGAAACTCG---GTGG
NM_004762.2       CCTCACTCACACTTTCTTCAATCCAGACCGAGAAGGCTGGCTATTGAAACTCGGAGGTGG
                  *****************************************************   ****

HsCD00296321      CAGGGTAAAGACTTGGAAGAGACGCTGGTTCATTCTGACTGACAACTGCCTTTACTACTT
NM_017456.2       CAGGGTAAAGACTTGGAAGAGACGCTGGTTCATTCTGACTGACAACTGCCTTTACTACTT
NM_004762.2       CAGGGTAAAGACTTGGAAGAGACGCTGGTTCATTCTGACTGACAACTGCCTTTACTACTT
                  ************************************************************

HsCD00296321      TGAGTATACCACGGATAAGGAGCCCCGTGGAATCATCCCTTTAGAGAATCTGAGTATCCG
NM_017456.2       TGAGTATACCACGGATAAGGAGCCCCGTGGAATCATCCCTTTAGAGAATCTGAGTATCCG
NM_004762.2       TGAGTATACCACGGATAAGGAGCCCCGTGGAATCATCCCTTTAGAGAATCTGAGTATCCG
                  ************************************************************

HsCD00296321      GGAAGTGGAGGACTCCAAAAAACCAAACTGCTTTGAGCTTTATATCCCCGACAATAAAGA
NM_017456.2       GGAAGTGGAGGACTCCAAAAAACCAAACTGCTTTGAGCTTTATATCCCCGACAATAAAGA
NM_004762.2       GGAAGTGGAGGACTCCAAAAAACCAAACTGCTTTGAGCTTTATATCCCCGACAATAAAGA
                  ************************************************************

HsCD00296321      CCAAGTTATCAAGGCCTGCAAGACCGAGGCTGACGGGCGGGTGGTGGAGGGGAACCACAC
NM_017456.2       CCAAGTTATCAAGGCCTGCAAGACCGAGGCTGACGGGCGGGTGGTGGAGGGGAACCACAC
NM_004762.2       CCAAGTTATCAAGGCCTGCAAGACCGAGGCTGACGGGCGGGTGGTGGAGGGGAACCACAC
                  ************************************************************

HsCD00296321      TGTTTACCGGATCTCAGCTCCGACGCCCGAGGAGAAGGAGGAGTGGATTAAGTGCATTAA
NM_017456.2       TGTTTACCGGATCTCAGCTCCGACGCCCGAGGAGAAGGAGGAGTGGATTAAGTGCATTAA
NM_004762.2       TGTTTACCGGATCTCAGCTCCGACGCCCGAGGAGAAGGAGGAGTGGATTAAGTGCATTAA
                  ************************************************************

HsCD00296321      AGCAGCCATCAGCAGGGACCCTTTCTACGAAATGCTCGCAGCACGGAAAAAGAAGGTCTC
NM_017456.2       AGCAGCCATCAGCAGGGACCCTTTCTACGAAATGCTCGCAGCACGGAAAAAGAAGGTCTC
NM_004762.2       AGCAGCCATCAGCAGGGACCCTTTCTACGAAATGCTCGCAGCACGGAAAAAGAAGGTCTC
                  ************************************************************

HsCD00296321      CTCCACGAAGCGACACTGAATCCACCCAGCTTTCTTGTAC
NM_017456.2       CTCCACGAAGCGACACTGA---------------------
NM_004762.2       CTCCACGAAGCGACACTGA---------------------
                  *******************                     

Pathways

NCI : Class I PI3K signaling events

Protein

SMART domain : Sec7 : Sec7 domain
SMART domain : PH : Pleckstrin homology domain.
UniProt : Q15438
HPRD : 01630

Clone

Cloning Information : 482663
HIP Master Clone ID : 400391
IMAGE ID : 100067335
Original Clone ID : FLH482663.01X

Organism


TAX_ID : 9606
Species Specific ID: 9267

Genome

Chromosome : 17
Map Location : 17q25
Ensembl : ENSG00000108669

Reagents

Labome : cytohesin-1-antibody